《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (7): 2165-2170.doi: 10.16431/j.cnki.1671-7236.2017.07.037

• 预防兽医 • 上一篇    下一篇

山东省猪腹泻病例中PEDV、TGEV和PRV的感染调查与分析

王淞1,2, 曾昊1, 陈智1,3, 焦安琪1, 张树金1,4, 于江1,3, 孙文博1,3, 张玉玉1,3, 陈蕾1,3, 杜以军1,3, 李俊1,3, 吴家强1,3, 王金宝1,2,3   

  1. 1. 山东省畜禽疫病防治与繁育重点实验室, 济南 250100;
    2. 青岛农业大学动物科技学院, 青岛 266109;
    3. 山东省农业科学院畜牧兽医研究所, 济南 250100;
    4. 聊城大学农学院, 聊城 252000
  • 收稿日期:2017-02-03 出版日期:2017-07-20 发布日期:2017-07-22
  • 通讯作者: 吴家强, 王金宝 E-mail:wujiaqiang2000@sina.com;wangjb@saas.ac.cn
  • 作者简介:王淞(1991-),男,山东诸城人,硕士生,研究方向:动物病毒学与免疫学,E-mail:zcwangsong@126.com;曾昊(1993-),女,山东潍坊人,研究方向:动物病原学检测与流行病学,E-mail:13287683711@163.com
  • 基金资助:

    泰山学者工程专项经费；山东省现代农业产业技术体系（SDAIT-08-06）；山东省农业重大应用技术创新项目；山东省农业科学院农业科技创新工程（CXGC2016B14）；山东省农业科学院重大科技成果培育计划（2014CGPY04）

Investigation and Analysis of Porcine Epidemic Diarrhea Virus, Transmissible Gastroenteritis Virus and Pseudorabies Virus of Swine Diarrhea Cases in Shandong Province

WANG Song1,2, ZENG Hao1, CHEN Zhi1,3, JIAO An-qi1, ZHANG Shu-jin1,4, YU Jiang1,3, SUN Wen-bo1,3, ZHANG Yu-yu1,3, CHEN Lei1,3, DU Yi-jun1,3, LI Jun1,3, WU Jia-qiang1,3, WANG Jin-bao1,2,3   

  1. 1. Shandong Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, China;
    2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
    3. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China;
    4. College of Agronomy, Liaocheng University, Liaocheng 252000, China
  • Received:2017-02-03 Online:2017-07-20 Published:2017-07-22

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摘要：

为了解山东省规模化猪场由猪流行性腹泻病毒（PEDV）、猪传染性胃肠炎病毒（TGEV）和猪伪狂犬病毒（PRV）引起猪病毒性腹泻的流行情况，自2014年1月至2016年12月，对来自山东省各地规模化猪场的猪腹泻病料（共3 035份）进行PCR检测。结果显示，PEDV、PRV和TGEV阳性率分别为67.49%、9.33%和3.29%；3年间，PEDV阳性率在2014年第四季度最低，为48.15%，2015年第四季度阳性率最高，为88.57%，2016年各季度阳性率相对2015年呈波动下降趋势；TGEV阳性率在2014年第四季度最高，为18.52%，2015年第三季度阳性率为15.38%，2016年第一季度阳性率为6.67%，其他季度未检测出阳性病料；PRV阳性率在2016年第二季度最高，为15.68%，除2014年第一季度未检出阳性病料外，2014年第二季度阳性率最低，为2.56%。通过对69份被动送检的病料进行PEDV、TGEV和PRV混合感染检测发现，这部分病料中PEDV、TGEV、PRV阳性率分别为86.96%、5.80%和37.68%；总单独感染率为69.57%，PEDV、TGEV和PRV单独感染率分别为57.97%、1.45%和10.14%；总混合感染率为30.43%，PEDV/PRV、PEDV/TGEV和TGEV/PRV混合感染率分别为26.09%、2.90%和1.45%；总单独感染率比总混合感染率高。结果表明，山东省存在PEDV、PRV和TGEV 3种病毒流行，存在PEDV/TGEV、TGEV/PRV和PEDV/PRV的混合感染，混合感染中主要为PEDV/PRV混合感染，不存在PEDV/PRV/TGEV的混合感染。目前PEDV是引起山东省猪病毒性腹泻的主要病因，本试验结果可为山东省猪病毒性腹泻的诊断和控制提供参考。

关键词: 猪流行性腹泻病毒; 猪传染性胃肠炎病毒; 猪伪狂犬病毒; 调查与分析

Abstract:

To understand porcine viral diarrhea prevalence in the large-scale pig farms of Shandong province, a total of 3 035 clinical samples were detected by PCR from January, 2014 to December, 2016.Those samples were detected for porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and pseudorabies virus (PRV). The results showed that the detection rate of PEDV, PRV and TGEV were 67.49%, 9.33% and 3.29%, respectively.During the past three years, the lowest detection rate of PEDV was 48.15% in the fourth quarter of 2014,and the highest was 88.57% in the fourth quarter of 2015.In 2016,the detection rate represented fluctuate declining compared with 2015.The highest positive rate of TGEV was 18.52% in the fourth quarter of 2014,and in the third quarter of 2015 was 15.38%.The lowest positive rate of TGEV was 6.67% in the first quarter of 2016 and TGEV was not detected in the other quarters. The highest detection rate of RPV was 15.68% in the second quarter of 2016,and the lowest was 2.56% in the second quarter of 2014,except the first quarter of 2014 that the PRV was 0. By detecting three kinds of viruses in 69 clinical samples collected passively, the results showed that the detection rate of PEDV,TGEV and PRV were 86.96%,5.80% and 37.68%,respectively. The total single infection rate was 69.57%,the single infection rates of PEDV,TGEV and PRV were 57.97%,1.45% and 10.14%, respectively;The total mixed infection rate was 30.43%,the mixed infection rates of PEDV/PRV,PEDV/TGEV and TGEV/PRV were 26.09%,2.90% and 1.45%, respectively;Obviously, the total single infection rate was higher than the total mixed infection rate. The results showed that the PEDV, PRV and TGEV were prevailing in Shandong province. There were PEDV/TGEV, TGEV/PRV, PEDV/PRV mixed infection, and the number of PEDV/PRV mixed infection was in the majority. However, there was no PEDV/PRV/TGEV mixed type infection. At present, PEDV was the major pathogen of porcine viral diarrhea and the test results could provide the reference to the diagnosis of porcine viral diarrhea.

Key words: porcine epidemic diarrhea virus; transmissible gastroenteritis virus; pseudorabies virus; investigation and analysis

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