关键词: 鹅; 大肠杆菌; 分离鉴定; 药敏试验

Abstract: In order to direct the treatment of goose E.coli disease and establish the foundation of preparation for the goose E.coli inactivated vaccine. The suspected cases of the goose E.coli disease were taken and separated and cultivated in MacConkey medium. The bacteria strains were obtained,and identificated by morphological assay,biochemistry assay,serotype assay,and animal experiments. Moreover,the identified E.coli were carried out by drug susceptibility test. The results showed that the strains of E.coli were pink,moist,smooth and neat in MaConkey medium,which were small,negative of Gram's staining,ends obtuse and many individual exists. The isolated bacteria were identified as E.coli,which animal experiment showed that the goose E.coli had higher infection rate and fatality rate. The 15 strains were identified by serotype assay: 4 O₃₅ strains,3 O₇₈ strains,3 O₂₆ strains,2 O₇₂ strains,1 O₁₀₉ strains,1 O₁₄₁ strain,1 O₂ strain. In vitro antibacterial experiment results showed that Doxycycline and Amoxicillin against the above E.coli had strong inhibited effect. The research provide the theoretical foundation and significant applical value for efficientively preventing and curing goose E.coli disease.

Key words: goose; E.coli; isolation and identification; drug sensitive test