›› 2011, Vol. 38 ›› Issue (1): 29-32.

• 生理生化 • 上一篇    下一篇

绿汁发酵液及青贮苜蓿中乳酸菌的分离筛选及鉴定

赵芸君1,李学森2,董志国2,安登弟3   

  1. (1.新疆畜牧科学院饲料研究所,新疆乌鲁木齐 830000;2.新疆畜牧科学院草业研究所,新疆乌鲁木齐 830000;3.新疆师范大学生命科学学院,新疆乌鲁木齐 830054)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-01 发布日期:2011-01-01

Selection of Lactic Acid Bacteria(LAB) from Previously Fermented Juice and Alfalfa Silage and its Character

ZHAO Yun-jun1,LI Xue-sen2,DONG Zhi-guo2,AN Deng-di3   

  1. (1.Feed Research Institute, Animal Science Academy of Xinjiang, Urumqi 830000,China;2.Grassland Research Institute, Animal Science Academy of Xinjiang, Urumqi 830000,China;3.College of Life and Science, Xinjiang Normal University, Urumqi 830054, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01

摘要: 苜蓿较难青贮,用青贮接种剂可提高苜蓿青贮的质量。本研究以pH的降低程度及稳定性,乳酸积累量多,产生香甜气味为指标,通过连续限制性培养,从绿汁发酵液、青贮苜蓿中共筛选出3株乳酸球菌和3株乳酸杆菌;该6株菌在72 h内均能使MRS液体培养基pH下降至3.2左右并维持稳定,在MRS固体培养基中培养散发出香甜气味,此6株菌可供开发苜蓿青贮接种剂作进一步研究。

关键词: 绿汁发酵液; 青贮苜蓿; 乳酸菌; 分离; 筛选

Abstract: Alfalfa is difficult to ensile, while silage inoculants could improving alfalfa silage quality.This article mainly aims at screening silage inoculant Lactic Acid Bacteria that could quickly debase and stabilize pH value and producing more lactic acid.A continuous restricted cultivation was carried on until three kinds of Lactic Acid Bacteria had been selected from previously fermented juice and three kinds of Lactic Acid Bacteria had been selected from alfalfa silage. These six kinds of Lactic Acid Bacteria could cause the mediums’ pH value debase to around 3.2 in 72 hours and emitted sugary fragrance when they were cultured in MRS.

Key words: previously fermented juice; alfalfa silage; Lactic acid bacteria (LAB); selection; character

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