›› 2011, Vol. 38 ›› Issue (1): 157-160.

• 遗传繁育 • 上一篇    下一篇

碱性磷酸酶特性及其应用的研究进展

王秋颖   

  1. (河北旅游职业学院,河北承德 067000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-01 发布日期:2011-01-01

Advances on Characteristic and Application of Alkaline Phosphatase

WANG Qiu-ying   

  1. (Hebei Tourism Vocational College, Chengde 067000,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01

摘要: 碱性磷酸酶(alkaline phosphatase,AP)是一种非特异性磷酸单酯酶,能催化磷酸单酯的水解反应,产生无机磷酸和相应的醇、酚或糖,也能催化磷酸基团的转移反应。AP广泛存在于微生物和动物体内,在磷生物地球化学循环过程中有重要作用,并广泛应用于诊断学、生物化学及分子生物学等领域。作者对碱性磷酸酶的研究历史、催化作用机制及其应用进行了综述。

关键词: 碱性磷酸酶; 非特异性磷酸单酯酶; 催化作用机制

Abstract: Alkaline phosphatase(AP) is a kind of hydrolase,which catalyzes the nonspecific hydrolysis of phosphate monoester to produce phosphate or transfer phosphate.AP exists widely in microbial and animal kingdom.AP plays a vital important role in phosphate cycle of world creatures,and has been used widely in the fields of diagnostics,biochemistry and molecular biology.In this paper,the history,the reaction mechanism and application of alkaline phosphatase was reviewed.

Key words: alkaline phosphatase; nonspecific phosphomonoesterase; reaction mechanism

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