山羊胚胎体外培养条件的优化

›› 2010, Vol. 37 ›› Issue (4): 146-149.

山羊胚胎体外培养条件的优化

刘羿羿¹，赵高平²，李燕⁴，白俊²，王申元²，巴特尔²，周欢敏³

（1.内蒙古农业大学生物工程学院，呼和浩特 010018； 2.内蒙古农业大学动物科学学院，呼和浩特 010018;3.内蒙古农业大学科技处，呼和浩特 010018；4.内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室，呼和浩特 010021）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20

Optimization of Goat Embryos in vitro Culture Conditions

LIU Yi-yi¹，ZHAO Gao-ping²，LI Yan⁴，BAI Jun²， WANG Shen-yuan²，BAR Ter²，ZHOU Huan-min³

（1.College of Biotechnology, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Animal Science and Medical Science, Inner Mongolia Agricultural University, Hohhot 010018, China；3.Science and Technology Agency, Inner Mongolia Agricultural University, Hohhot 010018, China;4.The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot 010021, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20

摘要/Abstract

摘要： 为优化山羊胚胎体外培养条件，本试验比较了含有3种不同来源血清（优质胎牛血清、发情山羊血清、前列腺素（PG）处理后发情山羊血清）的M199对卵母细胞成熟效果的影响，成熟率分别为65.95%、49.2%、76.47%，差异极显著（P＜0.01）；本试验还分别采用了SOFaa、CR1aa+输卵管上皮细胞共培养，以及改进的DMEM/F12发育体系培养受精卵，结果发现，SOFaa、CR1aa+输卵管上皮细胞的发育培养系统得到的囊胚率为29.62%、24.73%，差异不显著(P＞0.05)，改进的DMEM/F12发育液囊胚率最高为48.42%，差异极显著（P＜0.01）。

关键词: 发情山羊血清; 囊胚率; 输卵管上皮细胞

Abstract: This experiment compared the effect of mature liquid that containing three different sources serum: high-quality fetal bovine serum, estrus goat serum, PG treated estrus goat serum, maturation rate were 65.95%, 49.2%, 76.47% （P＜0.01）respectively, the difference was extremely significant; compared SOFaa, CR1aa+ oviduct epithelial cells, and improved DMEM/F12 development system and found that, the blastocyst rate of SOFaa, CR1aa+ oviduct epithelial cells were 29.62%, 24.73%, difference was not significant(P＞0.05), the DMEM/F12 system had higher blastocyst development rates 48.42%, the difference was very significant（P＜0.01）.

Key words: estrus goat serum; blastocyst rate; oviduct epithelial cells

中图分类号:

S814

刘羿羿;赵高平;李燕;白俊;王申元;巴特尔;周欢敏. 山羊胚胎体外培养条件的优化[J]. , 2010, 37(4): 146-149.

LIU Yi-yi;ZHAO Gao-ping;LI Yan;BAI Jun;WANG Shen-yuan;BAR Ter;ZHOU Huan-min. Optimization of Goat Embryos in vitro Culture Conditions[J]. , 2010, 37(4): 146-149.

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