›› 2009, Vol. 36 ›› Issue (5): 120-122.

• 遗传繁育 • 上一篇    下一篇

山羊线粒体DNA D-loop区遗传多样性研究

武建亮1,肖宇航1,2,刘文忠1   

  1. (1.山西农业大学动物科技学院, 太谷 030801; 2.山西晋中市财政局, 晋中 030600)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 刘文忠

Genetic Diversity of mtDNA D-loop in Goats

WU Jian-liang1, XIAO Yu-hang1,2, LIU Wen-zhong1   

  1. (1.College of Animal Science and Technology,Shanxi Agricultural University, Taigu 030801, China;2.The Bureau of Finance, Jinzhong 030600, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: LIU Wen-zhong

摘要: 本研究利用PCR-SSCP技术对8个山羊品种分析了线粒体DNA D-loop区5′端部分序列的遗传多样性。378个个体共检测到A、B、C、D、E、F、G 7种单倍型,平均的单倍型比例为1.9%。单倍型多样度以太行黑山羊最高,为0.8003;洪洞奶山羊最低,为0.5772,但也属于较高的遗传多样性。结果表明,8个山羊品种均有较高的遗传多样性。

关键词: 山羊; 线粒体DNA; PCR-SSCP; 遗传多样性

Abstract: The genetic diversity of mitochondrial DNA D-loop 5′-terminal partial sequences of 378 individuals in eight Chinese goat (Capra hiruc) breeds were analysised by PCR-SSCP technology. The results show that seven haplotypes (A, B, C, D, E, F and G) with the percentage of 1.9% in 378 animals were obtained in eight populations. The haplotype diversity was higher as 0.8003 for the Taihang black goat, and lower as 0.5772 for the Hongtong Milking goat, The results showed that the genetic diversity of Chinese goats is abundant.

Key words: goat; mitochondrial DNA; PCR-SSCP; genetic diversity

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