›› 2009, Vol. 36 ›› Issue (3): 45-47.

• 生物技术 • 上一篇    下一篇

不同日龄大白猪骨桥蛋白基因的表达研究

杨国伟,张冬杰,汪晓鸿,王文涛,刘娣   

  1. (黑龙江省农业科学院, 哈尔滨 150086)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20
  • 通讯作者: 刘娣

Research on Expression of Landrace OPN Gene on Different Days

YANG Guo-wei, ZHANG Dong-jie, WANG Xiao-hong, WANG Wen-tao, LIU Di   

  1. (HeilongJiang Academy of Agricultural Sciences, Harbin 150086,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: LIU Di

摘要: 本研究采用RT-PCR方法对大白猪的骨桥蛋白(osteopontin,OPN)基因在1、7、90、180、270和360日龄的心、肝、胃、脾、肾、肺、大肠、小肠、肌肉、子宫、卵巢共11个组织的表达情况进行了研究。结果表明,OPN基因是一个广谱表达基因,在所检测的11个组织、6个时间点上均表达,但是存在时间和空间上的变化。据此推测该基因的生物功能非常广泛。

关键词: 大白猪; 骨桥蛋白基因; RT-PCR; 表达

Abstract: Expression level of OPN gene in Landrace heart, liver, stomach, spleen, kidney, lung, large intestine, small intestine, muscle, uterus and ovary on 1 d, 7 d, 90 d, 180 d, 270 d and 360 d were studied by RT-PCR method. The result showed that OPN gene was a broad-spectrum expression gene, it had been detected in eleven tissues on six time point, but existed spatio-temporal change, so presumed that its biology function was very abroad.

Key words: Landrace;  OPN gene RT-PCR; expression

中图分类号: