›› 2008, Vol. 1 ›› Issue (7): 66-69.

• 遗传繁育 • 上一篇    下一篇

草原红牛改良群体HFABP基因SNP及其与肉质性状的相关分析

文力正1,赵玉民2,姜昊1,张国梁2,戴立胜1,赵志辉3 ,陈承祯1,张嘉保1   

  1. 1.吉林大学实验动物中心,长春 130062; 2.吉林省农业科学院,长春 130000;3.吉林大学畜牧兽医学院,长春 130062
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-20 发布日期:2008-07-20

Single Nucleltide Polymorphism of HFABP Gene and Correlation Analysis with Meat Quality Traits in Red Steppe Crossed Herds

WEN Li-zheng1, ZHAO Yu-min2, JIANG Hao1, ZHANG Guo-liang2, DAI Li-sheng1,ZHAO Zhi-hui3, CHEN Cheng-zhen1,ZHANG Jia-bao1   

  1. 1. Laboratory Animal Center, Jilin University, Changchun 130062,China;2.Jilin Academy of Agriculture Sciences, Changchun 130000,China; 3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20

摘要: 试验采用PCR-SSCP的方法对含1/2利木赞血缘的改良草原红牛的H-FABP基因5’-调控区进行SNP分析,并分析其多态性与改良草原红牛肉质性状的相关性。结果表明:改良草原红牛H-FABP基因5’-调控区存在多态位点,在136位点发生1个T碱基的插入突变,在142位点,发生了G→A转换。相关分析结果显示剪切力在AA和AB、BB基因型间差异极显著(P<0.01),AB与BB基因型间差异不显著(P>0.05),A基因对剪切力有负效应,其余肉质性状在不同基因型间差异均不显著(P>0.05)。表明HFABP基因对改良草原红牛肉质嫩度有显著影响。

关键词: HFABP; PCRSSCP; SNP; 肉质性状; 草原红牛

Abstract: PCR-SSCP was used in this study to detect H-FABP 5’UTR polymorphisms in 16 Red Steppe hybrids crossed with Limousin. The correlationship was carried out between different genotype and meat quality.The results showed that 5’UTR of H-FABP gene exist polymorphisms in Red Steppe Crossed Herds, base T was inserted in 136 bp and the transformation of G→A in 142 bp. A genotype was negatively to the tenderness trait. The difference between AA with AB and BB was extremely significant (P<0.01) in tenderness, but AB and BB was no significant difference(P>0.05).The difference between genotype with the other meat quality was not significant (P>0.05). The effect was very significant of H-FABP gene to tenderness traits in Red Steppe Crossed Herds.

Key words: H-FABP; PCRSSCP; SNP; meat quality; Red Steppe

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