›› 2008, Vol. 1 ›› Issue (3): 35-38.

• 遗传繁育 • 上一篇    下一篇

山羊BMP15基因FecX-L突变的检测

肖杰文1,储明星2,李学伟1,方丽2   

  1. 1.四川农业大学动物科技学院,雅安 625014;2.中国农业科学院北京畜牧兽医研究所,中国农业科学院家养动物遗传资源与种质创新重点开放实验室,北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-20 发布日期:2008-03-20

Detection of the FecX-L Mutation of BMP15 Gene in Goats

XIAO Jie-wen1,CHU Ming-xing2,LI Xue-wei1, FANG Li2
  

  1. 1.College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, China; 2. The Key-Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS, Institute of AnimalScience, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-20 Published:2008-03-20

摘要: 采用PCR-SSCP方法在高繁殖力山羊品种(济宁青山羊、波尔山羊和文登奶山羊)和低繁殖力山羊品种(内蒙古绒山羊、安哥拉山羊和辽宁绒山羊)中检测骨形态发生蛋白15(bone morphogenetic protein 15,BMP15)基因的FecX-L突变,同时研究该基因突变对济宁青山羊高繁殖力的影响。结果表明这6个山羊品种都没有发生与Lacaune绵羊相同的FecX-L突变(C53Y)。可见BMP15基因中影响Lacaune 绵羊高繁殖力的突变位点对济宁青山羊的高繁殖力没有显著影响。

关键词: 山羊; 高繁殖力; 骨形态发生蛋白15基因; FecX-L突变; PCR-SSCP

Abstract: The FecXL mutation of BMP15 gene was detected in both high fecundity breeds (Jining Grey, Boer and Wendeng Dairy goats) and low fecundity breeds (Inner Mongolia Cashmere, Angora and Liaoning Cashmere goats) by PCR-SSCP. The results indicated that none of the six goat breeds carried the same FecX-L mutation (C53Y) of the BMP15 gene as Lacaune sheep. So, the FecX-L mutation of BMP15 gene that had significant effect on fecundity of Lacaune sheep had no significant effect on prolificacy of Jining Grey goats. 

Key words: goat; prolificacy; BMP15 gene; FecX-L mutation; PCR-SSCP

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