AsiaⅠ型口蹄疫病毒灭活疫苗毒株不同宿主系传代中VP1基因的遗传变异研究

›› 2008, Vol. 1 ›› Issue (12): 65-70.

AsiaⅠ型口蹄疫病毒灭活疫苗毒株不同宿主系传代中VP1基因的遗传变异研究

朱晶晶^1，2，盛卓君³，符子华¹，易忠¹，魏玉荣¹，黄炯¹，马文革¹，胡俊³ ，朱刚⁴ ，徐亚萍⁴

1. 新疆畜牧科学院兽医研究所，乌鲁木齐 830000； 2.新疆农业大学，乌鲁木齐 830052；  3. 新疆动物防疫监督总站，乌鲁木齐 830000； 4.新疆天康畜牧生物技术股份有限公司，乌鲁木齐 830032

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-12-20 发布日期:2008-12-20

tudies on Genetic Variation of VP1 Genes of AsiaⅠ FMDV Inactivated Vaccine Strains from Serial Passages in Different Hosts

ZHU Jingjing1,2, SHENG Zhuojun3, FU Zihua1, YI Zhong1,WEI Yurong1, HUANG Jiong1,MA Wenge1,HU Jun3,ZHU Gang4,XU Yaping4

1. Veterinary Research Institute, Xinjiang Academy of Animal Sciences,Urumqi 830000,China；2. Xinjiang Agriculture University, Urumqi 830052,China;3. Xinjiang General Station of Animal Epidemic Prevention，Urumqi 830000，China；4. Xinjiang Tiankang Husbandary Biological Technique Company Ltd，Urumqi 830032 , China

Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

摘要/Abstract

摘要： 口蹄疫病毒结构蛋白VP1参与构成病毒粒子的主要中和抗原位点，是4种结构蛋白中最易发生变异的。在病毒传代过程中，对VP1基因进行遗传变异分析是口蹄疫疫苗研制中不可或缺的环节。为此，作者扩增了经不同宿主系（乳鼠、BHK₂₁细胞）连传不同代次的AsiaⅠ型毒株的VP1基因，并对其进行遗传变异分析，毒株间核苷酸同源性为99.4%~99.8%，推导氨基酸序列同源性为98.6%~100%；制苗毒株经过不同宿主系有限传代后，与传代前的原毒(MF1)相比，VP1基因未发生大的变异，主要抗原位点较稳定，说明以此种方式获得的制苗毒株制备的灭活疫苗是稳定的，适用于该毒株流行区域内相关家畜的免疫预防。

关键词: FMDV; Asia I型; 灭活疫苗毒株; VP1基因; 遗传变异分析

Abstract: The VP1 gene of FMDV contains major antigenic determinant and has the highest mutation rate in four structural proteins. Analysis of genetic variation during the passage is indispensable for vaccine research. This paper was studied on the sequence analysis of the VP1 gene of inactivated vaccine strains of FMDV which was generated in different hosts such as suckling mouse and BHK21 cell. The result demonstrated that the homologies among VP1 gene sequences were 99.4% to 99.8%，and the homologies among the deduced amino acid sequences were 98.6% to 100%. Compared with the MF1, VP1 gene and the main antigenic sites were conserved. So it is stable for producing inactivated vaccine and suitable to immune prevention of related livestock in prevailed area.

Key words: FMDV; type AsiaⅠ; strain of inactivated vaccine; VP1 gene; analysis of genetic variation

朱晶晶;;盛卓君;符子华;易忠;魏玉荣;黄炯;马文革;胡俊;朱刚;徐亚萍. AsiaⅠ型口蹄疫病毒灭活疫苗毒株不同宿主系传代中VP1基因的遗传变异研究[J]. , 2008, 1(12): 65-70.

ZHU Jingjing;;SHENG Zhuojun;FU Zihua;YI Zhong;WEI Yurong; HUANG Jiong;MA Wenge;HU Jun;ZHU Gang;XU Yaping. tudies on Genetic Variation of VP1 Genes of AsiaⅠ FMDV Inactivated Vaccine Strains from Serial Passages in Different Hosts[J]. , 2008, 1(12): 65-70.

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