›› 2007, Vol. 34 ›› Issue (9): 81-83.

• 疾病防治 • 上一篇    下一篇

鸡传染性支气管炎病毒HN99株血清学和免疫学特性的研究

高文明,崔保安,李新生,魏战勇,徐端红   

  1. 河南农业大学牧医工程学院,郑州 450002
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-20 发布日期:2007-09-20

The Immunological Properties and Serotyping of Infectious Bronchitis Virus HN99 Strain

GAO Wenming,CUI Baoan,LI Xinsheng,WEI Zhanyong,XU Duanhong
  

  1. College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

摘要: 在鸡胚肾细胞上对HN99株病毒进行了克隆纯化,经检测无外源病原污染。应用血清交叉中和试验确定了IBV HN99株的血清型,并用免疫保护试验进行了验证,结果证明HN99株与T株属同一血清型,与TJ、SD、湖北等地方分离株有一定的相关性。不同免疫剂量的效力试验结果表明用0.2 ml剂量的HN99灭活苗接种试验鸡,能够对HN99攻毒提供100%的保护,证明HN99株具有良好的免疫原性。

关键词: 鸡传染性支气管炎病毒; HN99株; 血清型; 免疫原性

Abstract:

IBV HN99strain was multiplied and purified by chick embryo kidney cell (CEKC), and the SPAFAS Jinan laboratory certified it was pure. Virus neutralization test (VN) revealed that the serotype of HN99strain was similar to IBV T strain. The inactivated vaccine of HN99 strain was prepared and it showed absolutely safety, moreover, chickens were immunized with the vaccine at different dose by muscle injection, three weeks later, 100 %protection were induced after challenging with HN99 strain, the results indicated that the IBV HN99 strain had fine Immunological Properties.

Key words: IBV; HN99 strain; serotype; immunological properties

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