›› 2007, Vol. 34 ›› Issue (2): 102-104.

• 疾病防治 • 上一篇    下一篇

鸡新城疫流行毒株与标准毒株的交叉免疫保护试验

刘燕1,荀来武2, 杨晓花1,何华川2
  

  1. 1.云南农业职业技术学院,昆明 650212;2.云南省家畜冷冻精液站,昆明 650212
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-20 发布日期:2007-02-20

A Cross Immunoprophylaxis Experiment with Epidemic Avian NDV Strain and Avian NDV Strain

LIU Yan1,XUN Laiwu2,YANG Xiaohua1,HE Huachuan2
  

  1. 1.Yunnan Agricultural Vocationaltechnical College,Kunming 650212,China; 2.Yunnan Domestic Animal Frozen Semen Station,Kunming 650212,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-20 Published:2007-02-20

摘要: 用新城疫(ND)分离强毒油佐剂灭活苗和NDV La Sota油佐剂灭活苗分别免疫母源抗体平均为2 log2的12日龄公雏。免疫后每隔1周采集血清,用微量血凝抑制试验检测HI抗体。免疫后21 d抗体水平达到峰值,抗体水平在6log2以上,维持30 d以上。免疫40 d后,用ND分离强毒株和NDVF48E9分别进行攻毒试验,2种疫苗的保护率均为100%,攻毒结果表明,ND分离强毒株和NDVF48E9株的免疫原性差异不显著。

关键词: 新城疫流行毒株; 新城疫标准毒株; 免疫保护

Abstract: 12 day old chicken, which averange titres of NDV antibodies is 2log2, was immunized with the oil adjuvant NDV velogenic strains vaccine and the oil adjuvant NDV La Sota strains vaccine. After the inoculability, the serum was pooled every week, and the titres of NDV antibodies was showed by the MicroHIA. The peak titres of NDV antibodies was appeared the 21th day, and the 6log2 high titres was keeping above 30 days. 40 days later in the immunization, the chicken were challenged by NDV velogenic isolates and NDVF48E9 isolates, and no chicken was infected. The percentage of protection from the two vaccines are 100%. The test of challege indicated that immunigenicity difference between the oil adjuvant NDV velogenic strains vaccine and the oil adjuvant NDV LaSda strains vaccine is not remarkable.

Key words: epidemic NDV strain; NDV strain; immunoprophylaxis

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