›› 2007, Vol. 34 ›› Issue (12): 74-76.

• 奶业专栏 • 上一篇    下一篇

奶牛临床型乳房炎病原菌的分离与鉴定

黄瑛,尹晓敏,李丽好,赵化芬,韩利方,杨华,赵德明   

  1. 中国农业大学动物医学院国家动物海绵状脑病实验室,北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-20 发布日期:2007-12-20

Isolation and Identification of Bovine Clinical Mastitis Pathogenic Bacteria

HUANG Ying, YIN Xiaomin, LI Lihao, ZHAO Huafen, HAN Lifang, YANG Hua,ZHAO Deming
  

  1. Laboratory of National Animal TSE,College of Veterinary Medicine, China Agriculture University, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-20 Published:2007-12-20

摘要: 奶牛乳房炎是奶牛常见病和多发病之一,其病因复杂,难以防制,给奶牛饲养业带来了巨大的经济损失。作者通过对某奶牛场的奶牛临床型乳房炎病原菌进行分离、鉴定,从105份样品中共分离出76株细菌,经培养特性、染色镜检、生化鉴定等试验,鉴定出无乳链球菌58株,占分离菌的76.32%;大肠杆菌18株,占分离菌的23.68%。本试验结果表明,无乳链球菌是奶牛临床型乳房炎的主要病原菌,其次是大肠杆菌。这为奶牛乳房炎的防制提供了重要依据。

关键词: 奶牛; 乳房炎; 无乳链球菌; 大肠杆菌; 分离; 鉴定

Abstract: Dairy mastitis happens frequently in cattle farm. Because of the complicated cause and no effective therapy, it has brought the cattle farms large economic loss. A series of experiments were designed to discover the reason why the disease happen in the cattle farm.76 bacterium were isolated from 105 samples of dairy mastitis, including 58 of streptococcus agalactiae(76.32%), 18 of escherichia coli(23.68%). The result demonstrates that Streptococcus agalactiae is main bacterium causing bovine clinical mastitis, the next is escherichia coli. It is important for the prevention and cure of bovine clinical mastitis.

Key words: dairy cow; mastitis; streptococcus agalactiae; E coli; isolation; identification

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