禽流感血清抗体与卵黄抗体消长规律比较研究

›› 2007, Vol. 34 ›› Issue (1): 103-105.

禽流感血清抗体与卵黄抗体消长规律比较研究

席文平1，李吉轩1，岳建新1，闫若潜2

1. 乾元浩郑州生物药厂，郑州 450061；2. 河南省动物疫病预防控制中心，郑州 450011

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-20 发布日期:2007-01-20

Studies on the Decreasing and Increasing Rule of Serum-antibody andYolk-antibody Titers in Laying Hens Vaccinated with Avian Influenza Oil-emulsion Killed Bivalent Vaccines

XI Wenping1, LI Jixuan1, YUE Jianxin1, YAN Ruoqian2

1. Zhengzhou Bio-pharmaceutical Factory , Qian Yuan Hao Biological Co Ltd, Zhengzhou 450061, China; 2. Henan Centre for Animal Diseases Control & Prevention，Zhengzhou 450011, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-01-20 Published:2007-01-20

摘要/Abstract

摘要： 应用禽流感病毒二价（H5、H9）油乳剂灭活疫苗免疫160日龄非免疫蛋鸡，免疫接种后分别于第0、7、14、21、28、35 d采集相应的鸡蛋和血清，采用血凝抑制(HI)试验监测血清及卵黄中H5、H9抗体的消长规律，结果表明：H5、H9血清抗体于免疫后7 d时开始产生，14 d时到中等水平，21 d时达到最高值，一直维持到35 d之后；而H5、H9卵黄抗体与血清抗体水平相比相对滞后7 d左右；卵黄抗体与血清抗体在28 d时均达到高峰值并一直维持到35 d之后，28 d后血清和卵黄抗体达到相同水平。本研究为临床上以禽流感卵黄抗体检测替代血清抗体检测及以后禽流感高免卵黄抗体的研究提供了依据和数据。

关键词: 禽流感; 血清抗体; 卵黄抗体; 消长; 检测

Abstract: The haemoglutination inhibition test were carried out for supervising the decreasing and increasing rule of serumantibody and yolkantibody titers of serum and yolk samples collected on 0、7、14、21、28、35 days from the 160days old preunimmunized laying hens after being immunized with avian influenza oilemulsion bivalent (H5 and H9) inactivated vaccines. The results revealed that the H5, H9 Serumantibody HI titer was first appeared on the 7th day, and the midHI titer was appeared on the 14th day, and the peak HI titer was maintained from the 21th day to and after the 35th day. Comparing with the Serum-antibody, the appearance of yolk-antibody titers was relatively hanged behind about 7 days, and the peak HI titers of both Serum-antibody and yolk-antibody were appeared on 28th day and sustained till and after 35th day, and the HI titers of yolk-antibody was also corresponding with that of Serum -antibody from the 28th day. Our results suggested that the HI titers of yolk-antibody could be used as the substitution of Serum -antibody after the lay hen immunized 28 days. The result was also laid a foundation for further studies and using the yolk antibodies (IgY) of AI. 

Key words: AI; serum-antibody; yolk-antibody; decreasing and increasing rule of HI titer; detection

中图分类号:

S852.4+3

席文平;李吉轩;岳建新;闫若潜. 禽流感血清抗体与卵黄抗体消长规律比较研究[J]. , 2007, 34(1): 103-105.

XI Wenping;LI Jixuan;YUE Jianxin;YAN Ruoqian . Studies on the Decreasing and Increasing Rule of Serum-antibody andYolk-antibody Titers in Laying Hens Vaccinated with Avian Influenza Oil-emulsion Killed Bivalent Vaccines[J]. , 2007, 34(1): 103-105.

[1]	廖思雨, 郑新, 戴琪, 许诗怡, 张天旭, 张秀桥, 桂春. 空心莲子草乙酸乙酯部位体内外抗H9N2亚型禽流感病毒活性的研究[J]. 中国畜牧兽医, 2024, 51(2): 759-769.
[2]	龙凤, 谢守玉, 崔鹏飞, 施开创, 韦显凯, 冯淑萍, 屈素洁, 陆文俊, 李剑锋, 尹彦文, 邓国华. 2016－2021年广西H9亚型禽流感病毒HA和NA基因遗传进化动态分析[J]. 中国畜牧兽医, 2023, 50(5): 1947-1958.
[3]	谢守玉, 王睿敏, 崔鹏飞, 施开创, 韦显凯, 冯淑萍, 龙凤, 屈素洁, 陆文俊, 黄金山, 黄凤梅, 温新瑞, 尹彦文, 邓国华. 2株海鸭源H3亚型禽流感病毒全基因组分子特征分析[J]. 中国畜牧兽医, 2023, 50(4): 1319-1328.
[4]	李琦, 田野, 黄涛, 刘存霞. 纳米疫苗在禽流感防控中的研究进展[J]. 中国畜牧兽医, 2023, 50(2): 684-692.
[5]	靳换, 涂敏, 史爱华, 赵蕾, 沈佳, 习硕, 张建伟, 章振华. H9亚型禽流感病毒分离鉴定及鸡新城疫、禽流感(H9亚型)、禽腺病毒病三联灭活疫苗对其免疫保护效果研究[J]. 中国畜牧兽医, 2023, 50(12): 5013-5021.
[6]	杨景, 张新宇, 梁志鹏, 程晴, 王聪颖, 池仕红, 袁生, 郭锦玥, 黄淑坚, 温峰. H9N2亚型禽流感病毒NP蛋白原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2022, 49(10): 3963-3971.
[7]	江宁, 尹航, 张岩威, 李紫馨, 池晓娟, 王松. H9N2亚型禽流感病毒与其他病原混合感染的研究进展[J]. 中国畜牧兽医, 2021, 48(9): 3447-3455.
[8]	尹彦文, 施开创, 孙文超, 谢守玉, 屈素洁, 陆文俊, 王露霞, 覃勇, 裴幸彪, 凌丹. 2013－2019年广西边境地区低致病性禽流感病毒监测分析[J]. 中国畜牧兽医, 2021, 48(8): 3002-3009.
[9]	贺维朝, 张会艳, 王浩, 赵青余, 汤超华, 张军民. 卵黄抗体提取方法及其在畜禽细菌性肠道疾病防治中的应用[J]. 中国畜牧兽医, 2021, 48(2): 640-649.
[10]	程邓芳, 梅晨, 刘娟, 王宏俊, 先宏. 中药复方对H9N2亚型禽流感病毒在MDCK细胞增殖的作用[J]. 中国畜牧兽医, 2021, 48(2): 650-657.
[11]	李青梅, 郭军庆, 孟泽锟, 李艳华, 刘肖, 石建州, 李鸽, 柴书军, 罗俊, 邓瑞广, 张改平. H9N2亚型禽流感病毒中和单克隆抗体的制备与应用[J]. 中国畜牧兽医, 2021, 48(10): 3761-3769.
[12]	苗海生, 寇美玲, 谢佳芮, 李乐, 李华春. 感染16型蓝舌病病毒后绵羊部分细胞因子消长特点研究[J]. 中国畜牧兽医, 2021, 48(1): 296-302.
[13]	程艺, 田辉, 张亚, 薛景景, 盛东北, 刘武杰, 王婉冰, 张盼涛, 田克恭. H9亚型禽流感病毒HF株的分离鉴定和免疫原性评价[J]. 中国畜牧兽医, 2020, 47(9): 2945-2952.
[14]	李佳佳, 李素华, 宋建领, 黄景军, 缪渊, 陈曦, 季佳. 2株云南H9N2亚型禽流感病毒HA和NA基因序列分析[J]. 中国畜牧兽医, 2020, 47(3): 873-883.
[15]	冯亚亚, 郭晶, 李玉保, 刘成, 李旭勇. 禽流感DNA疫苗研究进展[J]. 中国畜牧兽医, 2020, 47(11): 3667-3675.