蟾蜍似矛球吻棘头虫的临床病例研究

doi:10.16431/j.cnki.1671-7236.2022.06.042

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (6): 2423-2430.doi: 10.16431/j.cnki.1671-7236.2022.06.042

• 临床兽医 • 上一篇

蟾蜍似矛球吻棘头虫的临床病例研究

黄潇航^1,2, 李诗艺^1,2, 李永霞^1,2, 李梦蕊^1,2, 贺金峪^1,2, 张龙^1,3, 刘道泉⁴, 江和基^1,2, 黄志坚^1,2, 殷光文^1,2

1. 福建农林大学动物科学学院(蜂学学院), 福州 350002;
2. 福建省动物药物工程实验室, 福州 350002;
3. 福建省农业科学院畜牧兽医研究所, 福州 350013;
4. 福州市动物疫病预防控制中心, 福州 350009

收稿日期:2021-11-11 出版日期:2022-06-05 发布日期:2022-05-27
通讯作者: 殷光文 E-mail:yinguangwen000@sina.com
作者简介:黄潇航,E-mail:365038453@qq.com。
基金资助:
福建农林大学科技创新专项基金（CXZX2020125B、CXZX2019070G）；福建农林大学优秀硕士基金（1122YS01004）

Clinical Case Study of Toad Infected with Sphaerirostris lanceoides

HUANG Xiaohang^1,2, LI Shiyi^1,2, LI Yongxia^1,2, LI Mengrui^1,2, HE Jinyu^1,2, ZHANG Long^1,3, LIU Daoquan⁴, JIANG Heji^1,2, HUANG Zhijian^1,2, YIN Guangwen^1,2

1. College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Fujian Animal Drug Engineering Laboratory, Fuzhou 350002, China;
3. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
4. Fuzhou Animal Disease Control Center, Fuzhou 350009, China

Received:2021-11-11 Online:2022-06-05 Published:2022-05-27

摘要/Abstract

摘要： 【目的】对1例中华蟾蜍多脏器寄生虫感染临床案例进行病理诊断并通过分子生物学鉴定其物种分类。【方法】对实验室饲养的1只消瘦、拒食后发生死亡的雌性中华蟾蜍进行解剖观察，病理组织学研究，采用蠕虫剖检法、结节压片法对分离获得的虫体进行形态学观察研究，并进行分子生物学鉴定。【结果】剖检发现蟾蜍心脏、肝脏等脏器及多系膜寄生棘头虫，未在蟾蜍体内发现线虫、吸虫等其他蠕虫寄生。HE染色结果显示，棘头虫的感染寄生造成了蟾蜍肺脏、肝脏等多脏器的机械性损伤、炎性反应，其中肠壁黏膜发生严重损坏脱落。通过PCR扩增其细胞色素c氧化酶亚基Ⅰ（cytochrome c oxidase subunit Ⅰ，COⅠ）基因片段，获得5株不同部位的目的虫株COⅠ基因目的片段，经对比发现，鉴定的棘头虫均为同一种棘头虫，且其GC含量在40.14%~40.83%之间。选取JS-01株进行NCBI-BLAST比对，与似矛球吻棘头虫（Sphaerirostris lanceoide，登录号：MG931943.1）相似性最高，为99.5%。借助分子生物学分析并结合形态学观察结果确定其为似矛球吻棘头虫。【结论】似矛球吻棘头虫的大量感染寄生造成了蟾蜍的死亡，本试验结果可为药用蟾蜍棘头虫病的防控提供临床症状、病理、分子生物学等方面的参考，对两栖动物似矛球吻棘头虫的诊断与研究具有参考价值。

关键词: 蟾蜍; 似矛球吻棘头虫; 病理组织学

Abstract: 【Objective】 A clinical case of Bufo gargarizans infection with parasite in multiple organs was clinically diagnosed, and its species classification was studied through molecular biological identification.【Method】 An emaciated female Bufo gargarizans raised in the laboratory died after food refusal was dissected and observed, and the histopathology was studied.The morphology of the isolated parasite was observed and studied by helminth autopsy and nodule pressing method, and the molecular biology was identified.【Result】 Autopsy study showed that the toad' heart, liver, other organs and multi-mesangium were infected by Acanthocephala, and no other common worms such as nematodes and trematodes were found in the toad.HE staining results showed that the infection of Acanthocephala caused mechanical damage and inflammatory reactions to toad' lung, liver and other organs, in which the mucosa of the intestinal wall was seriously damaged and detached.Cytochrome c oxidase subunit Ⅰ (COⅠ) gene was amplified by PCR, five COⅠ gene segments of the species of interest were obtained from different parts, and the Acanthocephala was identified by comparison with the same species in GenBank, the GC contents ranged from 40.14% to 40.83%, and the similarity of JS-01 strain was the closest to Sphaerirostris lanceoides (accession No.:MG931943.1), which was 99.5%.By means of molecular biological analysis and morphological observation, it was determined to be Sphaerirostris lanceoides.【Conclusion】 A large number of infection and parasitism of Sphaerirostris lanceoides caused the death of toads.The results of this experiment could provide reference for the prevention and control of echinocephalosis in medicinal toads in terms of clinical symptoms, pathology and molecular biology, and had reference value for the diagnosis and research of Sphaerirostris lanceoides in amphibians.

Key words: toads; Sphaerirostris lanceoides; histopathology

中图分类号:

S852.73⁺2

黄潇航, 李诗艺, 李永霞, 李梦蕊, 贺金峪, 张龙, 刘道泉, 江和基, 黄志坚, 殷光文. 蟾蜍似矛球吻棘头虫的临床病例研究[J]. 中国畜牧兽医, 2022, 49(6): 2423-2430.

HUANG Xiaohang, LI Shiyi, LI Yongxia, LI Mengrui, HE Jinyu, ZHANG Long, LIU Daoquan, JIANG Heji, HUANG Zhijian, YIN Guangwen. Clinical Case Study of Toad Infected with Sphaerirostris lanceoides[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2423-2430.

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蟾蜍似矛球吻棘头虫的临床病例研究

Clinical Case Study of Toad Infected with Sphaerirostris lanceoides

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