加味平胃口服液提取工艺研究

doi:10.16431/j.cnki.1671-7236.2022.06.041

摘要/Abstract

摘要： 【目的】确定加味平胃口服液的提取工艺。【方法】采用L₉（3⁴）正交试验法对提取工艺进行研究。对含有挥发油的药材进行挥发油提取，药渣再混合其余药材进行水煎煮提取。在挥发油提取工艺中首先进行饮片粒度考察，选择合适的饮片大小，在此基础上选定溶剂倍量（A）、浸泡时间（B）和煎煮时间（C）3个因素，每个因素各取3个水平，进行正交试验；以挥发油的提取量为评价指标，对组方中苍术、厚朴、陈皮、木香和枳壳的挥发油提取工艺进行探索研究，并进行3次工艺验证。在水煎煮提取工艺中选定溶剂倍量（A）、煎煮时间（B）和提取次数（C）3个因素，每个因素各取3个水平，进行正交试验；以柚皮苷含量和苦参总碱含量（苦参碱+氧化苦参碱总含量）作为评价指标，对水煎煮提取工艺进行探索研究，并进行了3次工艺验证。【结果】饮片粒度筛选结果显示，1~2 cm的饮片更合理。影响挥发油提取因素大小为煎煮时间（C）>浸泡时间（B）>溶剂倍量（A），挥发油提取工艺为8倍水，煎煮10 h；影响水煎煮提取因素大小为提取次数（C）>煎煮时间（B）>溶剂倍量（A），水煎煮提取工艺为8倍水，煎煮1.5 h，提取3次。【结论】本研究通过试验验证得出了加味平胃口服液提取的具体方法、流程等，该工艺提取效率高、制备过程稳定可靠，可达到量产的工艺标准，具有重要的实用价值。

关键词: 正交试验; 提取工艺; 挥发油; 柚皮苷; 苦参碱

Abstract: 【Objective】 The study was aimed to determine the extraction process of Jiawei Pingwei oral liquid.【Method】 The L₉(3⁴) orthogonal test method was used to conduct in-depth research on the extraction process.The volatile oil of medicinal materials containing volatile oil was extracted, and the drug residue was mixed with other medicinal materials for decoction and extraction.In the extraction process of volatile oil, firstly, the particle size of decoction pieces was investigated and the appropriate size of decoction pieces was selected.On this basis, three factors were selected:Solvent volume (A), soaking time (B) and decocting time (C).Three levels were taken for each factor for orthogonal test.Taking the extraction amount of volatile oil as the evaluation index, the extraction process of volatile oil from Atractylodes macrocephala, Magnolia officinalis, tangerine peel, Aucklandia and Fructus aurantii in the prescription was explored and studied, and the process was verified for three times.In the process of water decocting and extracting, three factors were selected:Solvent times (A), decocting time (B) and extraction times (C), and three levels were taken for each factor for orthogonal test.Taking the content of naringin and the total alkali content of matrine (total content of matrine and oxymatrine) as evaluation indexes, the water decoction extraction process was explored and studied, and the process was verified for 3 times.【Result】 Particle size screening results of pieces showed that 1-2 cm pieces were more reasonable.The verification analysis showed that the factors affecting the extraction of volatile oil were decocting time (C) > soaking time (B) > solvent times (A).The extraction process of volatile oil was 8 times water and decocting for 10 h.The factors affecting the water decoction extraction were extraction times (C) > decocting time (B) > solvent times (A).The water decocting extraction process was 8 times water, decocted for 1.5 h, and extracted 3 times.【Conclusion】 This study obtained the specific extraction method and process of Jiawei Pingwei oral liquid through experimental verification.The process had high extraction efficiency, stable and reliable preparation process, could meet the process standard of mass production, and had important practical value.

Key words: orthogonal test; extraction process; volatile oil; naringin; matrine

中图分类号:

S853.7

陈昱骁, 李焕娟, 席晓花, 郝洁, 周德刚, 司红彬. 加味平胃口服液提取工艺研究[J]. 中国畜牧兽医, 2022, 49(6): 2414-2422.

CHEN Yuxiao, LI Huanjuan, XI Xiaohua, HAO Jie, ZHOU Degang, SI Hongbin. Study on Extraction Process of Jiawei Pingwei Oral Liquid[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2414-2422.

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