苦参碱对多重耐药表皮葡萄球菌整合子intⅠ和dfrA的影响

doi:10.11843/j.issn.1671-7236.2015.03.009

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (3): 563-568.doi: 10.11843/j.issn.1671-7236.2015.03.009

苦参碱对多重耐药表皮葡萄球菌整合子intⅠ和dfrA的影响

贾芳, 杨江流, 邢立群, 刘文慧

河套学院医学院, 巴彦淖尔 015000

收稿日期:2014-09-16 出版日期:2015-03-20 发布日期:2015-03-30
通讯作者: 刘文慧 E-mail:995373615@qq.com
作者简介:贾芳(1980-),女,内蒙古乌兰察布人,硕士,讲师,研究方向:微生物与基因工程,E-mail:jiafang198011@163.com
基金资助:
内蒙古自治区高等学校科学研究项目(2013NJZY3383)

Effects of Matrine on intⅠ and dfrA of Integron in Clinical Multidrug Resistant Staphylococcus epidermidis Isolates

JIA Fang, YANG Jiang-liu, XING Li-qun, LIU Wen-hui

Medical College, Hetao University, Bayannaoer 015000, China

Received:2014-09-16 Online:2015-03-20 Published:2015-03-30

摘要/Abstract

摘要： 为探索苦参碱对多重耐药表皮葡萄球菌(Staphylococcus epidermidis,SE)整合子整合酶(intⅠ)和二氢叶酸还原酶(dfrA)的影响,试验通过纸片扩散法筛选多重耐药菌株、以肉汤稀释法测定苦参碱对SE的最低抑菌浓度(MIC)和最佳逆转条件;通过普通PCR法测定苦参碱对SE整合子intⅠ和dfrA基因碱基序列的影响;通过荧光定量PCR法测定苦参碱对SE整合子intⅠ和dfrA mRNA表达量的影响,结果显示苦参碱亚浓度作用表皮葡萄球菌后对intⅠ、dfrA基因序列无影响;苦参碱对intⅠ mRNA表达量具有抑制作用,而对dfrA mRNA表达量影响不大,结果表明在SE体内苦参碱可从mRNA水平上抑制SE intⅠ的表达量.

关键词: 苦参碱; 表皮葡萄球菌; 整合子; 整合酶; 二氢叶酸还原酶

Abstract: To explore the effects of matrine on intⅠ and dfrA of integron in clinical multidrug resistant Staphylococcus epidermidis isolates.The K-B method was used to screen multidrug resistant isolates.The broth dilution method was applied to measure the minimum inhibitory concentration (MIC) and the best reversal condition.The ordinary PCR was applied to detect effect of matrine on intⅠ and dfrA gene sequences and Real-time PCR was applied to detect effect of matrine on intⅠ and dfrA mRNA expression levels.The ordinary PCR results verified that matrine had no effects on int Ⅰ and dfrA gene sequences.The Real-time PCR results demonstrated that int Ⅰ gene expression was down-regulated in presence of matrine rather than dfrA gene.Integron mRNA expression quantity of Staphylococcus epidermidis was decreased by matrine in vivo.

Key words: matrine; Staphylococcus epidermidis; integron; intⅠ; dfrA

中图分类号:

S852.61⁺1

贾芳, 杨江流, 邢立群, 刘文慧. 苦参碱对多重耐药表皮葡萄球菌整合子intⅠ和dfrA的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 563-568.

JIA Fang, YANG Jiang-liu, XING Li-qun, LIU Wen-hui. Effects of Matrine on intⅠ and dfrA of Integron in Clinical Multidrug Resistant Staphylococcus epidermidis Isolates[J]. , 2015, 42(3): 563-568.

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苦参碱对多重耐药表皮葡萄球菌整合子intⅠ和dfrA的影响

Effects of Matrine on intⅠ and dfrA of Integron in Clinical Multidrug Resistant Staphylococcus epidermidis Isolates

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