中国首例兔出血症病毒2型(RHDV2/b/GI.2)的鉴定及病理学观察

doi:10.16431/j.cnki.1671-7236.2021.01.038

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (1): 348-355.doi: 10.16431/j.cnki.1671-7236.2021.01.038

中国首例兔出血症病毒2型(RHDV2/b/GI.2)的鉴定及病理学观察

肖璐¹, 于吉锋¹, 林毅¹, 周泷², 郭志强¹, 谢晶¹, 岳建国³, 叶勇刚¹, 曹冶¹, 李兴玉¹, 潘梦¹, 叶健强¹, 李敏³, 康润敏¹

1. 四川省畜牧科学研究院动物遗传育种四川省重点实验室, 成都 610066;
2. 西南民主大学生命科学与技术学院, 成都 610066;
3. 成都市动物疫病预防控制中心, 成都 610066

收稿日期:2020-05-28 出版日期:2021-01-20 发布日期:2021-01-15
通讯作者: 康润敏 E-mail:78157881@qq.com
作者简介:肖璐(1991-),女,四川什邡人,硕士,研究方向:动物传染病与分子生物学,E-mail:yaanxiaolu@163.com;于吉锋(1982-),男,山东济南人,硕士,研究员,研究方向:动物疫病防控,E-mail:1652662593@qq.com
基金资助:
四川省财政运行专项（SASA2020CZYX006）；四川省应用基础项目（2019YJ0562）

First Detection of a Type 2 Rabbit Hemorrhagic Disease Virus (RHDV2/b/GI.2) in China and Its Pathological Observation

XIAO Lu¹, YU Jifeng¹, LIN Yi¹, ZHOU Long², GUO Zhiqiang¹, XIE Jing¹, YUE Jianguo³, YE Yonggang¹, CAO Ye¹, LI Xingyu¹, PAN Meng¹, YE Jianqiang¹, LI Min³, KANG Runmin¹

1. Sichuan Provincial Key laboratory of Animal Breeding and Genetics, Sichuan Animal Science Academy, Chengdu 610066, China;
2. College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China;
3. Animal Disease Prevention and Control Center of Chengdu City, Chengdu 610041, China

Received:2020-05-28 Online:2021-01-20 Published:2021-01-15

摘要/Abstract

摘要： 本试验旨在鉴定四川金堂某兔场疑似兔出血症病毒2型（RHDV2）感染疫情的病原，并分析病兔的病理组织学变化。利用血凝试验和RT-PCR检测病死兔内脏组织中的病原，取病变组织制作病理切片，观察分析各组织的病理组织学变化，同时应用病兔肝脏悬液感染幼兔，分析该毒株的致病力。血凝试验结果显示，所采集病死兔肝脏样品能凝集人"O"型血红细胞；RT-PCR扩增及测序结果显示，多对引物均能从样品中扩增出RHDV2特异性条带；病理组织学观察结果显示，病兔多脏器严重出血、肿胀，淋巴细胞和中性粒细胞大量浸润，气管黏膜、肝脏、肺脏出血尤为严重；动物试验结果显示，该毒株毒力较强，含毒肝脏悬液能在24 h内迅速致死幼兔。本研究经临床诊断、核酸检测及测序证实了此次疫情确由RHDV2感染引起，动物试验和病理组织学观察表明该毒株毒力较强，可引发脏器严重出血，造成病兔急性死亡，RHDV2的出现提示病毒的跨境传播情况不容乐观，应引起更大的重视。

关键词: 兔瘟; 兔出血症病毒2型(RHDV2/b/GI.2); 病理组织学; 致病力

Abstract: The purpose of this study was to identify the suspected etiology of Rabbit hemorrhagic virus type 2 (RHDV2) infection in a rabbit farm in Jintang,Sichuan province,and to analyze the pathological histological changes of the infected rabbits.Hemagglutination test (HA) and RT-PCR were used to detect and identify the pathogens of the rabbit,and then pathological sections were taken from the diseased tissue to observe and analyze the histopathological changes of each tissue.At the same time,liver suspension was used to infect young rabbits,and the virulence of this strain was analyzed.The results of HA showed that liver samples collected from diseased and dead rabbits could agglutinate human "O" red blood cells.RT-PCR and sequencing results showed that RHDV2 specific bands were amplified from the samples with multiple pairs of primers.The histopathology microscopic examination revealed that the rabbit displayed systemic hemorrhagic and internal swelling with infiltration of large lymphocytes and neutrophils,and the hemorrhagic in liver,lung,and tracheal mucosa were the most serious.The animal test results showed that this strain was highly virulent,and the liver suspension containing RHDV2 was lethal to young rabbits and caused death in 24 h.In this study,clinical diagnosis,nucleic acid testing and sequencing confirmed that the outbreak was caused by RHDV2 infection.Animal tests and histopathological observation showed that this strain was virulent,which could cause serious bleeding organs,resulting in acute death of the sick rabbit.The emergence of RHDV2 indicated that the cross-border spread of virus was not optimistic,and it should be seriously monitored.

Key words: rabbit hemorrhagic disease; Rabbit hemorrhagic disease virus type 2(RHDV2/b/GI.2); histopathology; virulence

中图分类号:

S855.3

肖璐, 于吉锋, 林毅, 周泷, 郭志强, 谢晶, 岳建国, 叶勇刚, 曹冶, 李兴玉, 潘梦, 叶健强, 李敏, 康润敏. 中国首例兔出血症病毒2型(RHDV2/b/GI.2)的鉴定及病理学观察[J]. 中国畜牧兽医, 2021, 48(1): 348-355.

XIAO Lu, YU Jifeng, LIN Yi, ZHOU Long, GUO Zhiqiang, XIE Jing, YUE Jianguo, YE Yonggang, CAO Ye, LI Xingyu, PAN Meng, YE Jianqiang, LI Min, KANG Runmin. First Detection of a Type 2 Rabbit Hemorrhagic Disease Virus (RHDV2/b/GI.2) in China and Its Pathological Observation[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 348-355.

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中国首例兔出血症病毒2型(RHDV2/b/GI.2)的鉴定及病理学观察

First Detection of a Type 2 Rabbit Hemorrhagic Disease Virus (RHDV2/b/GI.2) in China and Its Pathological Observation

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