纳米银对猪链球菌体外抗菌活性及其生物膜形成的影响

doi:10.16431/j.cnki.1671-7236.2022.03.040

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (3): 1172-1180.doi: 10.16431/j.cnki.1671-7236.2022.03.040

纳米银对猪链球菌体外抗菌活性及其生物膜形成的影响

刘宝玲^1,2, 李春玲¹, 蒋智勇¹, 杨冬霞¹, 张昆丽¹, 楚品品¹, 勾红潮¹, 蔡汝健¹

1. 广东省农业科学院动物卫生研究所, 广东省畜禽疫病防治研究重点实验室, 农业农村部兽用药物与诊断技术广东科学观测实验站, 广州 510640;
2. 仲恺农业工程学院动物科技学院, 广州 510225

收稿日期:2021-08-23 出版日期:2022-03-05 发布日期:2022-03-03
通讯作者: 蔡汝健 E-mail:466866569@qq.com
作者简介:刘宝玲,E-mail:745292679@qq.com。
基金资助:
广东省科技计划项目（2016B020202004）；科技创新战略专项资金（高水平农科院建设）（R2019YJ-YB2005、R2020QD-047）；创建市县农科所联系专家制项目

Effects of Silver Nanparticles on the Activity of Streptococcus suis and Its Biofilm Formation in vitro

LIU Baoling^1,2, LI Chunling¹, JIANG Zhiyong¹, YANG Dongxia¹, ZHANG Kunli¹, CHU Pinpin¹, GOU Hongchao¹, CAI Rujian¹

1. Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province of Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received:2021-08-23 Online:2022-03-05 Published:2022-03-03

摘要/Abstract

摘要： 【目的】研究纳米银（silver nanoparticles，AgNPs）对多重耐药猪链球菌（Streptococcus suis，S.suis）的体外抗菌活性及其生物膜形成的影响，为解决猪链球菌的耐药问题提供参考。【方法】以5株临床分离的多重耐药猪链球菌（S1018、S894、S786、S815和S844）为研究对象，采用微量肉汤稀释法测定AgNPs对5株猪链球菌的最小抑菌浓度（minimum inhibitory concentration，MIC）和最小杀菌浓度（minimum bactericidal concentration，MBC），通过测定AgNPs作用下细菌的生长速率以及不同浓度AgNPs对猪链球菌的抑制率来评价AgNPs的抗菌活性；通过结晶紫染色法测定各菌株生物膜的形成能力和6.25、12.5、25、50 μg/mL AgNPs对5株多重耐药猪链球菌菌株生物膜形成的影响。【结果】 AgNPs对S1018株的MIC和MBC均为12.5 μg/mL，对其余4株多重耐药猪链球菌的MIC和MBC均为25.0 μg/mL；AgNPs对多重耐药猪链球菌的抗菌活性好，25~100 μg/mL AgNPs对5株菌的抑制率可以达到96.60%~99.70%，在1/2MIC、1MIC和2MIC AgNPs作用下细菌生长速率明显受到抑制。5株多重耐药猪链球菌均能形成生物膜，6.25、12.5、25、50 μg/mL AgNPs对5株多重耐药猪链球菌菌株生物膜形成的抑制率为17.98%~45.58%。【结论】 AgNPs对多重耐药猪链球菌的活性和生物膜形成均具有抑制作用。

关键词: 纳米银; 多重耐药; 猪链球菌; 生物膜

Abstract: 【Objective】 The effects of silver nanoparticles (AgNPs) on the in vitro antibacterial activity and biofilm formation of multidrug resistant Streptococcus suis were studied to provide reference for solving the drug resistance problem of Streptococcus suis.【Method】 Five clinically isolated multi-drug resistant Streptococcus suis (S1018, S894, S786, S815 and S844) were used as the research objects.The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of AgNPs against Streptococcus suis were determined by micro broth dilution method.The antibacterial activity of AgNPs was evaluated by measuring the growth rate of Streptococcus suis under the action of AgNPs and the inhibition rate of different concentrations of AgNPs on Streptococcus suis.Furthermore, the biofilm formation ability of each strain and the effect of AgNPs with concentrations of 6.25, 12.5, 25 and 50 μg/mL on biofilm formation of 5 multi-drug resistant Streptococcus suis strains were evaluated by crystal violet staining.【Result】 The MIC and MBC of AgNPs against S1018 strain were both 12.5 μg/mL, and the MIC and MBC of AgNPs against the other four multi-drug resistant Streptococcus suis strains were all 25.0 μg/mL.AgNPs had good antibacterial activity against multi-drug resistant Streptococcus suis.The inhibition rate of AgNPs on 5 strains of Streptococcus suis could reach 96.60%-99.70% at the concentration of 25-100 μg/mL, and the growth rate of bacteria was significantly inhibited under the action of 1/2MIC, 1MIC and 2MIC AgNPs.5 strains of multi-drug resistant Streptococcus suis could form biofilms, and the inhibition rates of AgNPs at concentrations of 6.25, 12.5, 25, 50 μg/mL on their biofilm formation were 17.98%-45.58%, respectively.【Conclusion】 AgNPs inhibited the activity and biofilm formation of multi-drug resistant Streptococcus suis.

Key words: silver nanoparticles; multi-drug resistance; Streptococcus suis; biofilm

中图分类号:

S852.61

刘宝玲, 李春玲, 蒋智勇, 杨冬霞, 张昆丽, 楚品品, 勾红潮, 蔡汝健. 纳米银对猪链球菌体外抗菌活性及其生物膜形成的影响[J]. 中国畜牧兽医, 2022, 49(3): 1172-1180.

LIU Baoling, LI Chunling, JIANG Zhiyong, YANG Dongxia, ZHANG Kunli, CHU Pinpin, GOU Hongchao, CAI Rujian. Effects of Silver Nanparticles on the Activity of Streptococcus suis and Its Biofilm Formation in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1172-1180.

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纳米银对猪链球菌体外抗菌活性及其生物膜形成的影响

Effects of Silver Nanparticles on the Activity of Streptococcus suis and Its Biofilm Formation in vitro

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