一株高抗氧化活性戊糖片球菌的筛选

doi:10.16431/j.cnki.1671-7236.2022.01.019

摘要/Abstract

摘要： [目的] 筛选具有高抗氧化性的乳酸菌并进行菌种鉴定，为缓解畜禽养殖过程中的氧化应激提供新型抗氧化微生态制剂。[方法] 以体外耐受过氧化氢氧化胁迫方法进行初筛，以体外对过氧化氢的耐受存活率为指标进行复筛，从27株菌株中筛选具有显著抗氧化能力的乳酸菌；然后以DPPH自由基（DPPH·）和羟自由基（HO·）清除能力、还原活性、抗脂质过氧化能力、总超氧化物歧化酶（total superoxide dismutase，T-SOD）和总抗氧化能力（total antioxidant capacity，T-AOC）等为指标评价乳酸菌的体外抗氧化性能。[结果] 从27株筛选菌株中最终确定了1株具有高抗氧化活性的乳酸菌C2-0327，其对DPPH·清除率为95.2%，HO·清除率达141.0%，还原活性达3 147 μmol/L（以L-半胱氨酸当量计），抗脂质过氧化率达82.9%，T-SOD活性和T-AOC分别达81.32和20.10 U/mL。经16S rDNA分子鉴定，该菌株为戊糖片球菌C2-0327。[结论] 筛选、鉴定得到1株高抗氧化活性乳酸菌，鉴定为戊糖片球菌C2-0327，可用于新型抗氧化微生态制剂的研制。

关键词: 乳酸菌; 抗氧化活性; 筛选; 戊糖片球菌

Abstract: [Objective] In this experiment, lactic acid bacteria with high antioxidant activity were screened and identified in order to provide a new antioxidant microecological agent for alleviating oxidative stress in the process of livestock and poultry breeding. [Method] Lactic acid bacteria with significant antioxidant capacity were screened from 27 strains, by establishing a primary screening method for tolerance to hydrogen peroxide oxidative stress, and taking the tolerance survival rate to hydrogen peroxide as the re-screening index. Then the antioxidant activity of lactic acid bacteria was evaluated by DPPH free radical (DPPH·) and hydroxyl free radical (HO·) scavenging ability, reducing activity, anti lipid peroxidation ability, total superoxide dismutase (T-SOD) and total antioxidant capacity (T-AOC) in vitro. [Result] A Lactobacillus C2-0327 with excellent antioxidant activity was screened from the 27 strains. Scavenging rates of DPPH· and HO· of this isolate C2-0327 were 95.2% and 141.0%, respectively. The reducing activities of this isolate reached 3 147 μmol/L (L-cysteine equivalent), and inhibition rate of lipid peroxidation was up to 82.9%. T-SOD activity and T-AOC were 81.32 and 20.10 U/mL, respectively. The strain was identified as Pediococcus pentosus C2-0327 by 16S rDNA molecular identification. [Conclusion] A strain of Lactobacillus with high antioxidant activity was screened and identified, which was Pediococcus pentosus C2-0327. The high antioxidant Pediococcus pentosus could be used for the development of new antioxidant probiotics.

Key words: lactic acid bacteria; antioxidant activity; screening; Pediococcus pentosu

中图分类号:

S816.7

汪祥燕, 辛国芹, 徐海燕, 谷巍, 兰江华, 郝木强, 冯刚. 一株高抗氧化活性戊糖片球菌的筛选[J]. 中国畜牧兽医, 2022, 49(1): 179-187.

WANG Xiangyan, XIN Guoqin, XU Haiyan, GU Wei, LAN Jianghua, HAO Muqiang, FENG Gang. Screening of a Strain of Pediococcus pentosus with High Antioxidant Activity[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 179-187.

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