鸡腺病毒12个血清型代表毒株Penton蛋白基因分析

doi:10.16431/j.cnki.1671-7236.2020.11.001

摘要/Abstract

摘要： 为了解鸡腺病毒（fowl adenovirus，FAdV）Penton基因的遗传进化规律，试验根据GenBank中已公布的各基因型序列设计特异性引物，通过PCR技术分别扩增12个血清型毒株的Penton基因，连接至pEASY-Blunt Simple Cloning Vector上进行序列测定，并将12个血清型毒株Penton蛋白的核苷酸序列与NCBI上已公布的标准毒株的核苷酸序列使用ClustalW法进行分析比对，绘制遗传进化树。结果显示，12种血清型Penton蛋白核苷酸序列与各血清型标准毒株同源性均在99.2%以上，其中FAdV-1、FAdV-2、FAdV-3、FAdV-4、FAdV-6、FAdV-7、FAdV-8a、FAdV-8b、FAdV-10、FAdV-11与对应标准株同源性高达100%，这进一步证实了实验室保存毒株与世界相应标准毒株的一致性。Penton蛋白虽然保守性较高，但不同种之间仍存在明显差异。FAdV-A1与其他种毒株同源性为70.6%~73.3%；FAdV-B5与其他种毒株同源性为70.6%~77.9%；FAdV-C同种不同血清型之间核苷酸同源性为99.6%，FAdV-C4与其他种之间同源性71.2%~73.4%；FAdV-D同种不同血清型之间核苷酸同源性为95.6%~98.7%，与其他种之间最高为85.3%；FAdV-E同种不同血清型之间核苷酸同源性为98.2%~99.4%，不同种间最高为85.3%。即相同种毒株之间差异较小，不同种毒株之间差异较大。依据Penton蛋白的核苷酸序列同样可以将FAdV分为A、B、C、D、E 5个种。本研究通过成功克隆FAdV 12种血清型Penton基因，并进行遗传进化分析，为今后对FAdV诊断、监测及毒株鉴定提供了参考。

关键词: 鸡腺病毒(FAdV); Penton蛋白; 序列分析

Abstract: In order to understand the genetic evolution of Penton protein of fowl adenovirus(FAdV),the specific primers of Penton were designed on the basis of published sequences of different genotypes on GenBank.Then Penton gene of 12 serotypes were amplified by PCR and constructed into pEASY-Blunt Simple Cloning Vector for sequencing.The nucleotide sequences of Penton protein of 12 serotypes were analyzed and compared with the nucleotide sequences of standard strains published on NCBI by ClustalW method to draw a genetic phylogenetic tree.The results showed that the nucleotide sequences of Penton protein of 12 serotypes had more than 99.2% homology with their respective standard strain,among which the homology of FAdV-1,FAdV-2,FAdV-3,FAdV-4,FAdV-6,FAdV-7,FAdV-8a,FAdV-8b,FAdV-10 and FAdV-11 were as high as 100%.This further confirmed the consistency between the laboratory preserved strains and the world standard strains.However,although Penton protein was highly conserved,there were still significant differences among different species.The nucleotide homology between FAdV-A1 and other species was 70.6%-73.3%,while that of FAdV-B5 was 70.6%-77.9%.The nucleotide homology between FAdV-C strains of different serotypes was 99.6%,while homology between FAdV-C4 and other species was 71.2%-73.4%.The nucleotide homology between FAdV-D strains of different serotypes was 95.6%-98.7%,while the highest homology between FAdV-D and other species was 85.3%.The nucleotide homology between FAdV-E strains of different serotypes was 98.2%-99.4%,while the highest homology between FAdV-E and other species was 85.3%.In conclusion,the homology difference between strains of the same species was small,and that of different species was significant.FAdV could be divided into A,B,C,D and E species according to nucleotide sequence of Penton protein.This study successfully cloned 12 serotypes Penton gene of FAdV and performed genetic evolution analysis,which laid the foundation for the diagnosis,monitoring and identification of FAdV in the future.

Key words: fowl adenovirus (FAdV); Penton protein; sequence analysis

中图分类号:

S852.65⁺9.1

宋佳诚, 侯力丹, 吴应凯, 杨亚茜, 李俊平. 鸡腺病毒12个血清型代表毒株Penton蛋白基因分析[J]. 中国畜牧兽医, 2020, 47(11): 3417-3426.

SONG Jiacheng, HOU Lidan, WU Yingkai, YANG Yaxi, LI Junping. Gene Analysis of Penton Protein of 12 Serotypes of Fowl Adenovirus[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3417-3426.

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