禽腺病毒血清4型贵州株全基因序列分析

doi:10.16431/j.cnki.1671-7236.2021.01.005

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (1): 44-54.doi: 10.16431/j.cnki.1671-7236.2021.01.005

禽腺病毒血清4型贵州株全基因序列分析

尹德晶¹, 张云丹¹, 岳筠³, 李乔斌¹, 何玲¹, 李涛³, 王开功^1,2, 程振涛^1,2

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州省动物疫病与兽医公共卫生重点实验室, 贵阳 550025;
3. 贵州省动物疫病预防控制中心, 贵阳 550008

收稿日期:2020-08-19 出版日期:2021-01-20 发布日期:2021-01-15
通讯作者: 王开功, 程振涛 E-mail:chengzhentao@sohu.com;kgwang@gzu.edu.cn
作者简介:尹德晶(1997-),女,贵州六盘水人,硕士生,研究方向:动物疫病,E-mail:654625128@qq.com
基金资助:
贵州省科技计划项目（黔科合[2020]1Y409号）；贵州省研究生教育创新计划项目（GZZ2017002）；贵州省科技计划项目（黔科合平台人才[2018]5253号）

Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou

YIN Dejing¹, ZHANG Yundan¹, YUE Jun³, LI Qiaobin¹, HE Ling¹, LI Tao³, WANG Kaigong^1,2, CHENG Zhentao^1,2

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Guizhou Key Laboratory of Animal Disease and Veterinary Public Health, Guiyang 550025, China;
3. Guizhou Center for Animal Disease Prevention and Control, Guiyang 550008, China

Received:2020-08-19 Online:2021-01-20 Published:2021-01-15

摘要/Abstract

摘要： 为了解禽腺病毒血清4型（FAdV-4）地方流行毒株的分子进化情况，基于实验室分离的2株FAdV-4贵州株GZ-BJ株和GZ-QL株，分别对2株FAdV-4毒株进行PCR分段扩增，扩增产物克隆至载体，提取质粒进行PCR和双酶切鉴定后筛选出重组质粒进行测序，将测序结果依次拼接得到病毒的全基因组，获得FAdV-4贵州株的全基因序列，并对其进行序列和遗传进化分析。结果显示，通过PCR分段扩增成功获得了2株FAdV-4贵州株（GZ-BJ株和GZ-QL株）的全基因序列，长度分别为43 352、43 723 bp，FAdV-4 GZ-BJ株全基因序列长度比FAdV-4 GZ-QL株短371 bp，少6个ORF（22K、putative 9.1 ku、u-exon、ORF17、ORF28、ORF42），二者的氨基酸同源性为57.1%。2株FAdV-4贵州株同国内外不同地区FAdV-4毒株核苷酸同源性在88.7%~100%，与FAdV-4经典毒株ON1比对，2株FAdV-4贵州株和国内FAdV-4分离株均缺失ORF19、ORF27、ORF30。系统进化树分析显示，2株FAdV-4贵州株GZ-BJ株和GZ-QL株仍属于Ⅰ群C种FAdV。研究结果表明，2株贵州株FAdV-4 GZ-BJ株和FAdV-4 GZ-QL株较国内外FAdV-4毒株均存在进化与突变，且FAdV-4 GZ-BJ株变化较大，但尚未改变其血清型，这为探索FAdV-4致病机理的分子机制研究提供依据。

关键词: 禽腺病毒血清4型(FAdV-4); 序列分析; 变异分析

Abstract: To comprehend the molecular evolution of fowl adenovirus serotype 4 (FAdV-4) endemic strains,based on FAdV-4 GZ-BJ and FAdV-4 GZ-QL strains isolated in the laboratory,segmented amplification was performed on the two FAdV-4 strains,the amplified product was cloned into the vector,the plasmid was extracted,PCR and double enzyme digestion were performed,and the recombinant plasmid was screened for sequencing.The sequencing results were sequentially spliced to obtain the whole genome of FAdV-4 Guizhou strain,and sequence and genetic evolution analysis.The results showed that the lengths of two FAdV-4 Guizhou strains,GZ-BJ and GZ-QL strains,were 43 352 and 43 723 bp,were obtained by PCR fragmented amplification.respectively,and the complete genes of FAdV-4 GZ-BJ strain was 371 bp shorter than the FAdV-4 GZ-QL strain,and 6 ORFs (22K,putative 9.1 ku,u-exon,ORF17,ORF28,ORF42),and the amino acid homology of them was 57.1%.The nucleotide homology of the two FAdV-4 Guizhou strains with FAdV-4 strains in different regions at home and abroad were 88.7%-100%.The FAdV-4 two Guizhou strains and domestic isolates lacked ORF19,ORF27,ORF30 that compared with the FAdV-4 classic strain ON1.Phylogenetic tree analysis showed that the two Guizhou strains FAdV-4 GZ-BJ and FAdV-4 GZ-QL still belonged to group Ⅰ species C fowl adenovirus.This research indicated that the two Guizhou strains FAdV-4 GZ-BJ strain and FAdV-4 GZ-QL strain had evolution and mutations compared with domestic and foreign FAdV-4 strains,and FAdV-4 GZ-BJ strain had changed greatly,but not yet changing its serotype and provided a basis for exploring the molecular mechanism of FAdV-4 virus pathogenic mechanism.

Key words: fowl adenovirus serotype 4 (FAdV-4); sequence analysis; variance analysis

中图分类号:

S852.65

尹德晶, 张云丹, 岳筠, 李乔斌, 何玲, 李涛, 王开功, 程振涛. 禽腺病毒血清4型贵州株全基因序列分析[J]. 中国畜牧兽医, 2021, 48(1): 44-54.

YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.

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禽腺病毒血清4型贵州株全基因序列分析

Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou

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