3株维氏气单胞菌耐药性及毒力基因的比较

doi:10.16431/j.cnki.1671-7236.2020.09.033

摘要/Abstract

摘要： 为探究不同地区的维氏气单胞菌（Aeromonas veronii）耐药性及毒力基因是否存在区别，本研究从生长形态、生化试验、药敏试验、人工感染试验、16S rDNA基因测序同源性分析及毒力基因等方面比较了草鱼源菌株GZCY2019、杂交鲟源菌株GZXY2018及斑点叉尾鮰源菌株GZBD2017的差异。结果显示，3株不同源维氏气单胞菌在鲜血平板上均为圆形、表面湿润光滑、边缘整齐半透明的灰白色菌落，菌落周围均有β溶血环；革兰氏染色镜检均为两端钝圆短小阴性杆菌；药敏试验显示，3株菌对丁胺卡那、多西环素等药物均敏感，而对复方新诺明、头孢他啶、多黏菌素B等药物均中度敏感，对其他药物则表现出不同程度的敏感性或者耐药性；人工感染试验显示，菌株GZCY2019与GZBD2017对小鼠累计死亡率为100%，菌株GZXY2018的累计死亡率为80%；16S rDNA同源性分析显示，菌株GZCY2019与菌株GZBD2017同源性为99.9%，二者与菌株GZXY2018同源性均为97.1%；毒力基因检测结果显示，3株菌均携带热不稳定性肠毒素（Alt）、溶血素（hlyA）与鞭毛（Fla）等毒力基因，而对细胞毒性肠毒素（Act）及其热稳定性肠毒素（Ast）毒力基因的携带则表现不同。本研究通过不同源维氏气单胞菌的对比发现，3株菌在生长形态、染色镜检结果、生化结果上基本相似，但耐药性和毒力基因的携带存在较大差异。

关键词: 草鱼; 杂交鲟; 斑点叉尾鮰; 维氏气单胞菌

Abstract: To find out whether there were differences in drug resistance and virulence genes of Aeromonas veronii (A.veronii) from different regions,this study compared morphology,biochemistry test,drug sensitive test,artificial infection test,16S rDNA gene sequencing and virulence genes homology analysis for grass carp source strain GZCY2019,hybrid sturgeon source strain GZXY2018 and Ictalurus punctatus source strain GZBD2017.The results showed that all the three strains of A.veronii from different sources presented round,moist and smooth gray and translucent colonies with neat edges on the blood plate,and the colonies were surrounded by β hemolytic rings.Gram staining showed that negative Bacillus crassus at both ends.Drug sensitivity test showed that the three strains were all sensitive to butylaminacana,doxycycline,but were all intermediary to compound xinnomin,ceftadime,polymyxin B and other drugs,while were at different degrees of sensitivity or resistance to other drugs.Artificial infection test showed that the cumulative death rates of GZCY2019 and GZBD2017 were both 100%,while the rate of GZXY2018 was 80%.The homology analysis of 16S rDNA showed that the homology between GZCY2019 and GZBD2017 was 99.9%,while the homology between the former two strains and GZXY2018 was 97.1%.Virulence gene test results showed that Alt,hlyA and Fla virulence genes were all carried,while cytotoxic enterotoxin Act and Ast virulence genes were different.In this study,through comparison of multiple experiments with different sources of A.veronii,it was found that the three strains were basically similar in growth morphology,staining microscopy and biochemical results,but there were significant differences in resistance and virulence genes.

Key words: grass carp; hybrid sturgeon; Ictalurus punctatus; Aeromonas veronii

中图分类号:

S855.3

张飘, 胡安东, 杨霞, 潘吉脉, 刘妍罕, 曾茂芹, 程振涛, 姜海波, 文明. 3株维氏气单胞菌耐药性及毒力基因的比较[J]. 中国畜牧兽医, 2020, 47(9): 2979-2987.

ZHANG Piao, HU Andong, YANG Xia, PAN Jimai, LIU Yanhan, ZENG Maoqin, CHENG Zhentao, JIANG Haibo, WEN Ming. Comparison of Drug Resistance and Virulence Genes of Three Strains of Aeromonas veronii[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2979-2987.

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