2种奶牛隐性乳房炎检测方法比较

doi:10.16431/j.cnki.1671-7236.2020.08.038

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (8): 2677-2683.doi: 10.16431/j.cnki.1671-7236.2020.08.038

2种奶牛隐性乳房炎检测方法比较

李海强¹, 杨大盛², 卢琦¹, 汤少勋²

1. 贵州大学动物科学学院, 贵阳 550025;
2. 中国科学院亚热带农业生态研究所, 动物营养生理与代谢过程湖南省重点实验室, 畜禽养殖污染控制与资源化技术国家工程实验室, 亚热带农业生态过程重点实验室, 长沙 410125

收稿日期:2020-01-17 出版日期:2020-08-20 发布日期:2020-08-15
通讯作者: 卢琦, 汤少勋 E-mail:luqi2556728@163.com;sxtang@isa.ac.cn
作者简介:李海强(1992-),男,甘肃陇西人,硕士生,研究方向:反刍动物营养,E-mail:1304025520@qq.com
基金资助:
国家重点研发计划（2016YFD0501206）；湖南省科技重大专项（2017NK1020）

Comparison of Two Methods for Detecting Recessive Mastitis in Cows

LI Haiqiang¹, YANG Dasheng², LU Qi¹, TANG Shaoxun²

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received:2020-01-17 Online:2020-08-20 Published:2020-08-15

摘要/Abstract

摘要： 文章旨在更准确地检测隐性乳房炎，为评价隐性乳房炎乳汁的变化提供参考。试验分别采用加州乳房炎检测（CMT）法与溴麝香草酚蓝检测（BTB）法对35头奶牛进行隐性乳房炎的检测，同时比较了2种测定方法下乳pH、乳血清淀粉样蛋白A（SAA）、乳成分及血液常规成分的差异。结果表明，2种检测方法中，CMT法检出阳性率（40%）高于BTB法（34.3%），但差异不显著（P>0.05）；而CMT法的吻合率（35.71%）低于BTB法（41.67%）。2种检测方法呈阳性的乳pH显著高于阴性组（P<0.05），BTB检测法呈阳性乳的体细胞数显著高于（P<0.05）阴性乳，而CMT法检测呈阳性的乳中体细胞数（SCC）的对数值比阴性乳高13.6%；BTB法检测呈阳性的SAA显著低于阴性组（P<0.05）。2组阳性与阴性组间奶牛血细胞成分无显著差异（P>0.05）。结论表明，2种检测方法中，CMT法比BTB法灵敏，但BTB法更准确。

关键词: 隐性乳房炎; 奶牛; CMT法; BTB法; 乳成分

Abstract: The aim of this paper was to detect recessive mastitis more accurately and to provide a reference for evaluating the change of recessive mastitis milk.The methods of California mastitis test (CMT) and bromine vanilla phenol blue test (BTB) were used in the present experiment to detect the recessive mastitis of 35 cows,and the pH,milk serum amyloid A (SAA),milk composition and blood cells composition were also detected and compared between two methods.The results showed that the positive rate detected by CMT method (40%) was higher than that of BTB method (34.3%),while the difference was not significant (P>0.05),and the match rate of CMT method (35.71%) was lower than that of BTB method (41.67%).The pH of milk in two positive groups were significantly higher (P<0.05) than that of negative group.The logarithms of somatic cell count of positive group detected by BTB test was significantly higher than that of negative group (P<0.05),which was numerically lower than that positive group detected by CMT test.The SAA content in the milk of positive group detected by BTB test was significantly lower than that of the negative group (P<0.05).There was no significant difference in the blood cell components among the two testing positive and negative groups (P>0.05).It was concluded that the CMT test was more sensitive than that of BTB test,while the BTB test was more accurate.

Key words: recessive mastitis; cows; CMT method; BTB method; milk composition

中图分类号:

S852.6

李海强, 杨大盛, 卢琦, 汤少勋. 2种奶牛隐性乳房炎检测方法比较[J]. 中国畜牧兽医, 2020, 47(8): 2677-2683.

LI Haiqiang, YANG Dasheng, LU Qi, TANG Shaoxun. Comparison of Two Methods for Detecting Recessive Mastitis in Cows[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2677-2683.

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2种奶牛隐性乳房炎检测方法比较

Comparison of Two Methods for Detecting Recessive Mastitis in Cows

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