太行鸡CYP19A1基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.05.023

摘要/Abstract

摘要： 试验旨在克隆蛋鸡CYP19A1基因，对其遗传结构进行生物信息学分析并探究其在太行鸡不同组织中及不同产蛋量个体中的表达情况。以河北省太行鸡为研究对象，通过设计引物对CYP19A1基因CDS区进行克隆测序，运用生物信息学软件对其功能结构进行预测。结果显示，CYP19A1基因含有一个1 509 bp的开放阅读框（ORF），编码502个氨基酸。同源性比对和系统发生树分析结果表明，太行鸡CYP19A1基因与鸭的同源性较高（90.7%），并且在不同物种间高度保守。对CYP19A1蛋白理化性质进行预测分析发现，该蛋白为亲水蛋白，蛋白分子式为C₂₆₀₂H₄₁₀₃N₆₇₅O₇₁₉S₃₆，分子质量为57.51 ku，理论等电点为5.99，其中含量最高的是亮氨酸（为14.0%）。蛋白质二级结构由α-螺旋、延伸链、β-折叠及无规则卷曲4种结构组成，所占比例分别为58.95%、2.18%、3.93%和34.93%。组织表达谱分析发现，CYP19A1基因在不同组织中均有表达，但其在卵巢中表达量最高。实时荧光定量PCR结果显示，CYP19A1基因在太行鸡高产组卵巢中的表达量显著高于低产组（P<0.05）。上述结果为研究太行鸡CYP19A1基因提供了重要的研究数据，为进一步挖掘太行鸡高产基因提供了参考。

关键词: 太行鸡; CYP19A1基因; 生物信息学分析; 表达模式

Abstract: This study was aimed to clone and analyze the CYP19A1 gene in laying hens by bioinformatics and investigate its expression in different tissues and egg production.The Taihang chicken in Hebei was taken as the research object.The CYP19A1 gene CDS region was cloned and sequenced by designing primers,and its functional structure was predicted by bioinformatics software.The results showed that the chicken CYP19A1 gene contained a 1 509 bp open reading frame (ORF) encoding 502 amino acids.The results of homology alignment and phylogenetic tree analysis indicated that the sequence of the Taihang chicken CYP19A1 gene was close to that of duck (90.7%).The physicochemical properties of CYP19A1 protein were predicted and analyzed.The structure formula of CYP19A1 protein was C₂₆₀₂H₄₁₀₃N₆₇₅O₇₁₉S₃₆ and the molecular mass was 57.51 ku.The theoretical isoelectric point was 5.99,with leucine being the highest abumdance.The CYP19A1 was hydrophilic protein.There was portions that could form alpha helix (58.95%),extended chain (2.18%),beta sheet (3.93%) and random coils (34.93%).The results of tissue expression profiling showed that the gene was expression in all the tissues,with the highest abundance in ovary.The results of Real-time PCR showed that the expression level of CYP19A1 gene in ovary issure of high-yield group was significantly higher than that of low- yield group (P<0.05).The above results provided important research data for studying the laying performance of the Taihang chicken.

Key words: Taihang chicken; CYP19A1 gene; bioinformation analysis; expression pattern

中图分类号:

S813.3

张辉, 张清阳, 李晨晨, 杨俊琦, 杜云良, 李凯扬, 刘玉芳. 太行鸡CYP19A1基因克隆及生物信息学分析[J]. 中国畜牧兽医, 2020, 47(5): 1489-1497.

ZHANG Hui, ZHANG Qingyang, LI Chenchen, YANG Junqi, DU Yunliang, LI Kaiyang, LIU Yufang. Cloning and Bioinformatics Analysis of CYP19A1 Gene in Taihang Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1489-1497.

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