河流型水牛TGF-β基因家族鉴定及其胚胎发育早期的表达分析

doi:10.16431/j.cnki.1671-7236.2020.05.021

摘要/Abstract

摘要： 本研究旨在鉴定河流型水牛中转化生长因子-β （transforming growth factor-β，TGF-β）基因家族成员，分析该家族成员在胚胎发育早期的表达情况，并讨论其可能的作用。根据人类中已鉴定的TGF-β基因家族信息，通过BLASTP在河流型水牛全基因组层面鉴定TGF-β家族成员，同时对牛、山羊和小鼠的TGF-β家族信息进行鉴定，结合5个物种中的TGF-β基因家族信息构建系统进化树。根据河流型水牛胚胎发育早期4个阶段（2-细胞、8-细胞、桑椹胚和囊胚期）的RNA-seq数据分析，通过FPKM（Fragment of Per Kilobase of exon model per Million mapped reads）值计算TGF-β家族成员的表达情况。结果显示，根据人类中的33个TGF-β基因，分别在河流型水牛、牛、山羊和小鼠的基因组中鉴定出32、23、32和26个TGF-β基因，几个物种中TGF-β基因家族成员数量相近且在系统进化树中分布均匀，说明该家族在各个物种中具有分布和功能的保守性。在河流型水牛的32个TGF-β基因中，21个TGF-β基因家族成员在胚胎发育早期不表达或极低剂量表达，6个分化抑制因子低剂量表达，5个高表达的TGF-β基因均有报道过与繁殖过程相关，说明在河流型水牛的胚胎发育早期只有部分成员（5/32）参与其中行使功能，绝大多数的TGF-β基因（27/32）并没有参与其中。本研究结果为河流型水牛中TGF-β基因家族研究提供了数据基础，并为TGF-β基因家族在胚胎发育早期的功能研究提供了理论依据。

关键词: 水牛; 胚胎发育早期; TGF-β基因家族; RNA-seq

Abstract: This study was aimed to identify the transforming growth factor-β (TGF-β) gene family in River buffalo,detect their expression profiling,and analyze the possible function of TGF-β gene in preimplantation embryo development.According to the reported TGF-β gene in human,TGF-β family members in River buffalo were identified at the whole genome level by BLASTP,the TGF-β gene information in Bos taurus,Capra hircus and Mus musculus were characterized.Phylogenetic tree of TGF-β gene family was constructed to elucidate evolutional relationship among these five species.The expression of TGF-β gene was calculated by Fragment of Per Kilobase of exon model per Million mapped reads (FPKM) based on RNA-seq data at four stages of preimplantation embryo development (2-cell,8-cell,morula and blastocyst).The results showed that there were 32,23,32 and 26 TGF-β gene in River buffalo,Bos taurus,Capra hircus and Mus musculus,respectively.The similar numbers of TGF-β gene among five species and uniform distribution in the phylogenetic tree indicated that the conservation of TGF-β gene distribution and function among different species.In 32 TGF-β gene of River buffalo,21 TGF-β gene did not express (or extremely low express) in preimplantation embryo development,6 TGF-β gene which had been identified as inhibitor,exhibited low expression level.5 TGF-β gene,which were involved in reproduction,showed high expression level,which suggested that only partial of TGF-β gene (5/32) rather than most of them (27/32) were involved in preimplantation embryo development of River buffalo.The results provided the foundation for the study of TGF-β gene family in River buffalo,and provided a theoretical basis for the functional analysis of TGF-β gene family in early embryonic development.

Key words: buffalo; preimplantation embryo development; TGF-β gene family; RNA-seq

中图分类号:

Q786

庞春英, 梁莎莎, 马小娅, 陆杏蓉, 段安琴, 邓廷贤, 黄韵琪, 梁贤威. 河流型水牛TGF-β基因家族鉴定及其胚胎发育早期的表达分析[J]. 中国畜牧兽医, 2020, 47(5): 1472-1480.

PANG Chunying, LIANG Shasha, MA Xiaoya, LU Xingrong, DUAN Anqin, DENG Tingxian, HUANG Yunqi, LIANG Xianwei. Identification of TGF-β Gene Family and Expression Analysis in Preimplantation Embryo Development of River Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1472-1480.

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