螺旋藻多糖与银杏叶提取物复方对四氧嘧啶模型小鼠的降血糖作用

doi:10.16431/j.cnki.1671-7236.2019.07.036

摘要/Abstract

摘要：

为研究复合螺旋藻多糖与银杏有效成分的降血糖作用，试验选用SPF级昆明种雄性小鼠，腹腔注射0.2 mL 200 mg/（kg·d）四氧嘧啶，连续5 d，断尾采血测定空腹血糖，其值大于11.1 mmol/L时表明造模成功。试验小鼠随机分为空白对照组（C组）、模型组（M组）、阳性对照组（CY组）、单一用药组（螺旋藻多糖组（P组）、银杏黄酮组（F组）、银杏内酯组（L组），复合用药组将螺旋藻多糖（PSP）与银杏叶有效成分（GBE）配制成1：1（CP₁组）、2：1（CP₂组）、1：2（CP₃组）的复合组，每组10只，共9组。对照和CY组每只小鼠灌服2.5 mg/（kg·d）格列本脲，其余各组按200 mg/（kg·d）灌服相应药物，每天1次，连续灌服30 d，测定小鼠血糖值及体重、脾脏指数、胸腺指数及肝糖原等指标。结果显示，与M组相比，用药组小鼠体重均极显著增加（P<0.01），复合用药组药效高于单一用药组；各用药组脾脏、胸腺指数均极显著升高（P<0.01）；肝糖原含量极显著增多（P<0.01）；血糖值极显著下降（P<0.01）；其中CP₂组的小鼠血糖值降幅最大，其降糖率为52.14%；肝糖原增加为61.88%。综上所述，螺旋藻多糖和银杏叶有效成分发生了协同增效作用，对由四氧嘧啶引起的高血糖小鼠有明显的降血糖作用。

关键词: 螺旋藻多糖; 银杏叶提取物; 四氧嘧啶; 小鼠; 降血糖

Abstract:

The aim of this experiment was to study the hypoglycemic effect of different proportions of polysaccharide from Soirulina platensis (PSP) and Ginkgo biloba extract (GBE).SPF Kunming male mice were selected for the experiment.Diabetic model was made by intraperitoneal injection of 0.2 mL at a concentration of 200 mg/(kg·d) for 5 consecutive days.The model was successfully constructed when the fasting blood glucose was greater than 11.1 mmol/L by tail-cut blood sampling.Then mice were randomly divided into blank control group,model group,positive control group,group P (PSP),group F (Ginkgo biloba flavone),group L(Ginkgo biloba lactones) and composite treatment groups that were PSP and GBE mixture of 1:1 (CP₁ group),2:1 (CP₂ group) and 1:2 (CP₃ group),10 mice in each group.Each mice in the control and CY groups was given 2.5 mg/(kg·d) glibenclamide,and mice in the other groups were given 200 mg/(kg·d) corresponding drugs once a day for 30 d continuously.The blood glucose value,body weight,spleen index,thymus index,liver glycogen and other indicators were measured.The results showed that compared with the model group,the body weight of mice in the drug groups was extremely significantly increased (P<0.01).The efficacy of compound drug groups was higher than that of single drug groups.Spleen and thymus indexes and liver glycogen content were extremely significantly increased (P<0.01),while the blood glucose level was extremely significantly decreased (P<0.01).Among them,the CP₂ group showed the greatest drop in blood glucose,and the hypoglycemic rate was 52.14% and the increase of hepatic glycogen was 61.88%.In conclusion,polysaccharide from Soirulina platensis and Ginkgo biloba effective components had synergistic effect on hyperglycemic mice induced by alloxan and had obvious hypoglycemic effect.

Key words: polysaccharide from Soirulina platensis (PSP); Ginkgo biloba extract (GBE); alloxan; mice; hypoglycemic

中图分类号:

R283

于蕾妍, 隋君霞, 李华涛, 董俊杰, 高善颂. 螺旋藻多糖与银杏叶提取物复方对四氧嘧啶模型小鼠的降血糖作用[J]. 《中国畜牧兽医》, 2019, 46(7): 2176-2182.

YU Leiyan, SUI Junxia, LI Huatao, DONG Junjie, GAO Shansong. Hypoglycemic Effect of Polysaccharide from Soirulina platensis and Ginkgo biloba Extract Compound on Alloxan-induced Model Mice[J]. , 2019, 46(7): 2176-2182.

参考文献

[1] LOOKER A C,EBERHARDT M S,SAYDAH S H.Diabetes and fracture risk in older US adults[J]. Bone,2016,82:9-15.
[2] 贺星,田红,徐颂,等.糖尿病治疗药物的研究进展[J].现代药物与临床,2009,24(3):129-133. HE X,TIAN H,XU S,et al.Research progress of diabetes drugs[J]. Drug & Clinic,2009,24(3):129-133.(in Chinese)
[3] 左绍远,罗华君,朱振宇.螺旋藻多糖对糖尿病小鼠抗氧化能力的影响[J].药物生物技术,2001,8(1):36-38. ZUO S Y,LUO H J,ZHU Z Y.Effect of polysaccharide from Soirulina platensis on anti-oxidation in experimental diabetic mice[J]. Pharmaceutical Bioechnology,2001,8(1):36-38.(in Chinese)
[4] 于蕾妍,姜忠玲,周颖,等.不同比例螺旋藻多糖与银杏叶提取物对小鼠抗疲劳作用的研究[J].中国兽药杂志,2018,52(7):72-77. YU L Y,JIANG Z L,ZHOU Y,et al.Anti-fatigue effect of different proportions of Spirulina polysaccharide and Ginkgo biloba on mice[J]. Chinese Journal of Veterinary Drug,2018,52(7):72-77.(in Chinese)
[5] 于蕾妍,邹本革,周颖,等.螺旋藻复合多糖与银杏提取物对小鼠耐缺氧能力的影响[J].动物医学进展,2017,38(4):72-74. YU L Y,ZOU B G,ZHOU Y.Effect of Spirulina complex polysaccharide on hypoxia tolerance in mice[J]. Progress in Veterinary Medicine,2017,38(4):72-74.(in Chinese)
[6] 钱志刚.螺旋藻多糖提取新工艺研究[J].淮海工学院学报,2000,9(2):50-52. QIAN Z G.A Study on the new process to separate polysaccharide from Spirulina platensis[J]. Journal of Huaihai Institute of Techology,2000,9(2):50-52.(in Chinese)
[7] 洪阁,李松年,马世堂,等.黄芪复方提取物对小鼠降血糖作用的研究[J].医学导报,2009,28(11):1444-1446. HONG G,LI S N,MA S T,et al.Research on hypoglycemin effect of the extracts from a Chinese traditional compound formula Huang Qi in mice[J]. Herald of Medicine,2009,28(11):1444-1446.(in Chinese)
[8] BENNETT W L,WILSON L M,BOLEN S,et al.Oral diabetes medications for adults with type 2 diabetes:An update[J]. Agency for Healthcare Research & Quality,2011,233(3):170-171.
[9] 岳颖,周珺,贾正平,等.影响肝糖代谢的中药活性成分抗糖尿病研究进展[J].医药导报,2018,37(4):465-469. YUE Y,ZHOU J,JIA Z P,et al.Research progress on anti-diabetes of traditional Chinese medicine active components affecting liver sugar metabolism[J]. Herald of Medicine,2018,37(4):465-469.(in Chinese)
[10] 贾少婷,殷文政.天然螺旋藻多糖的提取纯化及降血糖活性实验研究[J],农产品加工学刊,2007,88(1):46-48. JIA S T,YIN W Z.Study on hypoglycemic effect of polysaccharide from spirulina[J]. Academic Periodical of Farm Products Processing,2007,88(1):46-48.(in Chinese)
[11] 贺玉琢.银杏叶类黄酮对大鼠食后血糖升高的抑制作用[J].国外医学中医中药分册,2005,27(5):305. HE Y Z.Inhibitory effects of flavonoid from Ginkgo bilboa leaves on hyperglycemia in rats after eating[J]. Foreign Medical Sciences,2005,27(5):305.(in Chinese)
[12] 廖璞,王淑琴,廖雪松,等.银杏叶提取物对糖尿病大鼠肾脏保护作用的实验研究[J].中国药房,2000,11(3):114-115. LIAO P,WANG S Q,LIAO X S,et al.An experimetal study of protective effect of Ginkgo bilboa leaves extract on renal lesions in diabetic rats[J]. China Pharmacy,2000,11(3):114-115.(in Chinese)
[13] 郭春生,孙晓红,葛蔚,等.高剂量复合螺旋藻多糖辐射保护作用研究[J].时珍国医国药,2008,19(8):1913-1914. GUO C S,SUN X H,GE W,et al.Antiradiation efflect of high dosage compound PSP[J]. Lishizhen Medicine and Materia Research,2008,19(8):1913-1914.(in Chinese)
[14] 于蕾妍,赵旭光,邹本革,等.低剂量复合螺旋藻多糖对小鼠耐缺氧能力的影响[J].黑龙江畜牧兽医,2017,9:202-204. YU L Y,ZHAO X G,ZOU B G,et al.Effects of low dosage compound polysacchairides from Spirulina patensis on hypoxia tolerance in mice[J]. Heilongjiang Animal Science and Veterinary Medicine,2017,9:202-204.(in Chinese)
[15] 张志娟,乔明霞,郝言芝,等.复合螺旋藻多糖对人肝癌7402细胞的抑制作用[J].陕西医学杂志,2009,38(3):279-281. ZHANG ZH J,QIAO M X,HAO Y Z,et al.Anti-tumor effects of compound polysacchairides from Spirulina patensis on 7402 cells[J]. Shanxi Medical Journal,2009,38(3):279-281.(in Chinese)
[16] 郝言芝,张志娟,乔明霞,等.复合螺旋藻多糖对人宫颈癌HeLa细胞的抑制作用[J].安徽农业科学,2009,37(8):3367-3368. HAO Y Z,ZHANG Z J,QIAO M X,et al.Inhibilory effects of composite PSP on HeLa cells of human cervical cancer[J]. Journal of Anhui Agricultural Sciences,2009,37(8):3367-3368.(in Chinese)
[17] 于蕾妍,郝言芝,乔明霞,等.复合螺旋藻多糖对人乳腺癌231细胞抑制作用的研究[J].安徽农业科学,2009,37(3):928-929. YU L Y,HAO Y Z,QIAO M X,et al.Inhibilory effects of compound polysacchairides from Spirulina patensis on human breast cancer cell line 231[J]. Journal of Anhui Agricultural Sciences,2009,37(3):928-929.(in Chinese)
[18] 乔明霞,于蕾妍,郝言芝,等.复合螺旋藻多糖对人胃癌MGC细胞的抑制作用[J].时珍国医国药,2009,20(6):1385-1386. QIAO M X,YU L Y,HAO Y Z,et al.Inhibilory effects of complex polysacchairides from Spirulina patensis on human gastric cancer MGC cells[J]. Li Shizhen Medicine and Materia Reseach,2009,20(6):1385-1386.(in Chinese)
[19] 陈志伟,唐超,葛蔚,等.中剂量复合螺旋藻多糖对小鼠S180肉瘤的抑制作用[J].天然产物研究与开发,2008,22(5):3-6. CHEN Z W,TANG C,GE W,et al.Inhibilory effects of middle dosage compound polysacchairides from Spirulina patensis on the sarcoma 180 of mice[J]. Research and Development of Natural Products,2008,22(5):3-6.(in Chinese)