幼龄仔猪PEDV感染肠道损伤模型的建立

doi:10.16431/j.cnki.1671-7236.2018.06.037

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (6): 1715-1721.doi: 10.16431/j.cnki.1671-7236.2018.06.037

幼龄仔猪PEDV感染肠道损伤模型的建立

李鹏¹, 吴梦郡¹, 余魁¹, 徐海旺¹, 吴涛^1,2, 易丹^1,2, 王蕾^1,2, 赵迪^1,2, 侯永清^1,2

1. 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉 430023;
2. 武汉轻工大学, 动物营养与饲料安全湖北省协同创新中心, 武汉 430023

收稿日期:2017-09-18 出版日期:2018-06-20 发布日期:2018-06-15
通讯作者: 侯永清 E-mail:houyq@aliyun.com
作者简介:李鹏(1991-),男,湖北襄阳人,硕士生,研究方向:营养生化与代谢调控,E-mail:1536698031@qq.com
基金资助:
国家重点研发计划专项（2016YFD0501210）；国家自然科学基金项目（31572416）；武汉轻工大学科研项目（2016J08）

The Establishment of the Intestinal Injury Model of PEDV Infection in Young Piglets

LI Peng¹, WU Mengjun¹, YU Kui¹, XU Haiwang¹, WU Tao^1,2, YI Dan^1,2, WANG Lei^1,2, ZHAO Di^1,2, HOU Yongqing^1,2

1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;
2. Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China

Received:2017-09-18 Online:2018-06-20 Published:2018-06-15

摘要/Abstract

摘要：

试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大），随机分为两个处理组：对照组和PEDV组，每个处理8个重复，每个重复1头猪。试验期为10 d，试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为10^4.5 TCID₅₀），对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1 g/kg体重），1 h后前腔静脉采血，屠宰取样，取十二指肠、空肠、回肠等组织样品，测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明：①PEDV感染显著降低了仔猪ADG（P<0.05），极显著提高了仔猪腹泻率（P<0.01）；②PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01），显著提高了十二指肠和空肠隐窝深度（P<0.05）；③PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01），极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明，口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。

关键词: 猪流行性腹泻病毒; 仔猪; 肠道损伤; 模型

Abstract:

This study was conducted to establish the intestinal injury model of porcine epidemic diarrhea virus (PEDV) infection in neonatal piglets.Sixteen crossbred healthy piglets (Ducoc×Landrace×Yorkshire,7-day-old neonatal pigs) were randomly allocated to control and PEDV groups.During a 10-day trial,all the pigs were fed the basal diet.On the 7th day,the infected pigs were orally administrated with 10^4.5TCID₅₀ of PEDV per pig,while the control pigs were given equal amount of sterile saline.On the 10th day,all pigs were orally gavaged with D-xylose (0.1 g/(kg·BW)).One hour later,blood was collected from jugular vein,and then all pigs were killed to obtain the small intestines.The average daily weight gain (ADG),diarrhea rate (DR),intestinal morphological structure,mucosal injury correlated gene mRNA level were measured.The results showed that:①PEDV infection increased the incidence of diarrhea (P<0.05),while reduced ADG (P<0.01);②PEDV infection decreased the plasma D-xylose content,villus height in the jejunum and ileum,the ratio of villus height to crypt depth in the duodenum,jejunum and ileum (P<0.01),and increased crypt depth in the duodenum and jejunum(P<0.05);③PEDV infection up-regulated the mRNA level of PEDV M gene in the jejunum (P<0.01),and down-regulated mRNA levels of villin and i-FABP in the jejunum (P<0.01).In conclusion,the intestinal injury model was successfully established with PEDV infection in neonatal piglets.

Key words: porcine epidemic diarrhea virus; piglets; intestinal injury; model

中图分类号:

S852.3

李鹏, 吴梦郡, 余魁, 徐海旺, 吴涛, 易丹, 王蕾, 赵迪, 侯永清. 幼龄仔猪PEDV感染肠道损伤模型的建立[J]. 《中国畜牧兽医》, 2018, 45(6): 1715-1721.

LI Peng, WU Mengjun, YU Kui, XU Haiwang, WU Tao, YI Dan, WANG Lei, ZHAO Di, HOU Yongqing. The Establishment of the Intestinal Injury Model of PEDV Infection in Young Piglets[J]. , 2018, 45(6): 1715-1721.

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幼龄仔猪PEDV感染肠道损伤模型的建立

The Establishment of the Intestinal Injury Model of PEDV Infection in Young Piglets

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