富氢生理盐水干预小型猪肝脏缺血再灌注合并肝脏部分切除损伤中内质网应激影响的研究

doi:10.16431/j.cnki.1671-7236.2018.06.034

摘要/Abstract

摘要：

为探究富氢生理盐水（hydrogen-rich saline，HRS）干预小型猪肝脏缺血再灌注合并肝脏部分切除损伤中内质网应激（endoplasmic reticulum stress，ERS）影响，试验选取4~6月龄、体重20~30 kg的健康巴马小型猪18头，随机分为3组，每组6只，分别为假手术组、模型组和HRS干预组。假手术组仅进行翻动肝叶，维持气腹3 h；模型组用腹腔镜手术建立右半肝脏缺血60 min合并左半肝脏切除模型；HRS干预组建立手术模型并于门静脉置管在再灌注前10 min及术后1、2、3 d经静脉注射10 mL/kg HRS。各组分别于术前、再灌注即刻、术后即刻、术后1 d、术后3 d经腹腔镜手术采取肝脏组织，进行HE染色检测，并检测肝脏组织中ERS参数：PKR样ER调节激酶（PERK）、需肌醇酶1（IRE1）、活化转录因子6（ATF6）、葡萄糖调节蛋白78（GRP78）、活化转录因子4（ATF4）、C/EBP转录因子（CHOP）mRNA的表达水平。结果显示，模型组、HRS干预组肝脏组织病理变化较假手术组严重，且与假手术组相比，模型组、HRS干预组ERS相关参数mRNA表达水平上调；HRS干预组肝脏组织病理变化较模型组轻微，且各项ERS相关参数mRNA表达水平均低于模型组。结果表明，缺血再灌注损伤（ischemia-reperfusion injury，IRI）能诱导肝脏ERS，导致肝脏损伤，而HRS可能是通过抑制过度的ERS从而减轻肝脏IRI。

关键词: 小型猪; 富氢生理盐水(HRS); 肝脏缺血再灌注; 内质网应激(ERS)

Abstract:

In order to study hydrogen-rich saline protects effects against liver ischemia-reperfusion injury and hepatectomy by inhibiting endoplasmic reticulum stress in miniature pig,18 3 to 4 months old and 20 to 30 kg body weight healthy miniature pigs were chosen and randomly divided into three groups:Sham group,model group and HRS group.Sham group only flipped the liver and maintained pneumoperitoneum for 3 h.Laparoscopic technique was employed to establish the model of hepatic ischemia-reperfusion combined with partial hepatectomy in model group.The surgical model was established in HRS group,and 10 mL/kg HRS was injected into the portal vein at 10 min before per-fusion and 1,2,3 d after surgery.Liver tissue samples were collected before operation,immediately after reperfusion,surgery,1 d after surgery and 3 d after surgery.HE staining and the mRNA expression of ERS-related parameters (PERK,IRE1,ATF6, GRP78,ATF4 and CHOP) in the liver were detected.The results showed that pathological changes of liver tissue in model group and HRS group were more serious than that in sham operation group,and ERS-related parameters mRNA level were increased in the model group and HRS group when compared with the sham group.In addition,the liver pathological damage in HRS group was significantly lighter than that in the model group,and all ERS-related parameters of mRNA levels were decreased in HRS group compared with the model group.Those results indicated that ischemia-reperfusion could induce liver ERS and injury,HRS could protect the liver from IRI by inhibiting ERS.

Key words: miniature pig; hydrogen-rich saline (HRS); hepatic ischemia-reperfusion; endoplasmic reticulum stress (ERS)

中图分类号:

S857.12

李辉, 葛延松, 白鸽, 张千振, 孔祥东, 孟伟静, 王洪斌. 富氢生理盐水干预小型猪肝脏缺血再灌注合并肝脏部分切除损伤中内质网应激影响的研究[J]. 《中国畜牧兽医》, 2018, 45(6): 1692-1699.

LI Hui, GE Yansong, BAI Ge, ZHANG Qianzhen, KONG Xiangdong, MENG Weijing, WANG Hongbin. Hydrogen-rich Saline Protects Effect Against Liver Ischemia-reperfusion Injury and Hepatectomy by Inhibiting Endoplasmic Reticulum Stress in Miniature Pig[J]. , 2018, 45(6): 1692-1699.

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