中国小型猪内源性反转录病毒研究进展与对策

doi:10.16431/j.cnki.1671-7236.2017.04.043

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1244-1248.doi: 10.16431/j.cnki.1671-7236.2017.04.043

中国小型猪内源性反转录病毒研究进展与对策

冯书堂^1,2, 马玉媛³, 夏颖¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 北京盖兰德生物科技有限公司, 北京 100007;
3. 军事医学科学院输血医学研究所, 北京 100850

收稿日期:2016-08-29 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 冯书堂 E-mail:fst508@sina.com
作者简介:冯书堂(1945-),男,河北大名人,研究员,博士生导师,研究方向:繁殖生物技术与近交系猪培育
基金资助:
国家"863"（2012AA020603）；国家转基因专项（2008ZX08012-002-05）；北京市科技成果转化项目（2015-02）

Research Progress and Countermeasures of Porcine Endogenous Retrovirus in Chinese Miniature

FENG Shu-tang^1,2, MA Yu-yuan³, XIA Ying¹

1. Institute of Animal Sciences, China Academy of Agricultural Sciences, Beijing 100193, China;
2. Grand Life Science and Technology Co., Ltd., Beijing 100007, China;
3. Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China

Received:2016-08-29 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

作者介绍了国内外猪内源性反转录病毒（PERV）研究进展，简述了中国不同品种小型猪PERV存在情况、PERV前病毒全基因克隆与序列分析、细胞水平鉴定方法、感染HEK293细胞研究平台的创建、猪A3F对PERV的抑制作用研究，以及从分子遗传学上揭示不同品系PERV特异性等创新性研究成果，分析了五指山小型猪近交系具有PERV基因拷贝少且没有PERV-C等特异性，以及展望培育中国PERV阴性猪新品系方法等研究，以期为人类异种器官移植和生物医用材料产品安全性研发，解决小型猪PERV疾病传播危险性提供科学对策和新的方向。

关键词: 中国小型猪; 猪内源性反转录病毒; 感染性克隆; 异种移植

Abstract:

The paper is a brief introduction of porcine endogenous retrovirus (PERV) innovative research results in the world and also expounds the passing PERV existence situation on different varieties of miniature pig, analyzes the PERV-virus gene cloning and sequence, appraises method on cell level, create the platform of infection HEK293 cells research, study on pigs A3F inhibition of PERV, and reveal the innovative research results on the specific molecular genetics in the different strain of PERV, analyzes the advantages of Wuzhishan miniature pig inbred line such as low gene copy of PERV,and there is no passing PERV-C specificity and as well as looking forward to cultivate the methods for new strain of PERV negative pigs. It will provide a scientific counter measure and new perspective to solve the spread of disease risk of miniature pig PERV and product safety for human xenotransplantation and biomedical materials of research and development.

Key words: Chinese miniature pig; porcine endogenous retrovirus; infectious clone; xenotransplantation

中图分类号:

S852.65⁺9.3

冯书堂, 马玉媛, 夏颖. 中国小型猪内源性反转录病毒研究进展与对策[J]. 《中国畜牧兽医》, 2017, 44(4): 1244-1248.

FENG Shu-tang, MA Yu-yuan, XIA Ying. Research Progress and Countermeasures of Porcine Endogenous Retrovirus in Chinese Miniature[J]. , 2017, 44(4): 1244-1248.

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中国小型猪内源性反转录病毒研究进展与对策

Research Progress and Countermeasures of Porcine Endogenous Retrovirus in Chinese Miniature

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