新疆伊犁地区马疱疹病毒1型ORF30基因序列分析

doi:10.16431/j.cnki.1671-7236.2018.01.008

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (1): 65-70.doi: 10.16431/j.cnki.1671-7236.2018.01.008

新疆伊犁地区马疱疹病毒1型ORF30基因序列分析

范斌¹, 王燕¹, 阿玛古丽·朱马汉², 冉多良¹, 刘建华¹, 加尔肯¹

1. 新疆农业大学动物医学学院, 家畜传染病实验室, 乌鲁木齐 830052;
2. 新疆畜牧科学院畜牧业经济与信息研究所, 乌鲁木齐 830011

收稿日期:2017-08-03 出版日期:2018-01-20 发布日期:2018-01-20
通讯作者: 加尔肯 E-mail:2672049884@qq.com
作者简介:范斌(1993-),男,陕西汉中人,硕士生,研究方向:传染病的预防及防治,E-mail:944327928@qq.com;王燕(1994-),女,江苏徐州人,本科,研究方向:基础兽医学,E-mail:1311156742@qq.com
基金资助:
新疆少数民族科技人才特殊培养计划科研项目（2016D0214）

Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang

FAN Bin¹, WANG Yan¹, Zhumahan·A², RAN Duoliang¹, LIU Jianhua¹, Jarhen¹

1. Laboratory of Animal Infectious Disease, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China;
2. Xinjiang Academy of Animal Husbandry Economics and Information Research Institute, Urumqi 830011, China

Received:2017-08-03 Online:2018-01-20 Published:2018-01-20

摘要/Abstract

摘要：

为研究马疱疹病毒1型（EHV-1-XJ2015）新疆伊犁分离株致病基因型，评估新疆EHV-1-XJ2015的潜在风险因素，本试验设计特异性引物，应用PCR技术扩增EHV-1-XJ2015分离株ORF30基因相应区域，连接至克隆载体，成功构建重组质粒pMD19-T-ORF30。测序结果表明，EHV-1-XJ2015毒株ORF30基因2 254 bp处为A，且编码天冬酰胺（N），分析证明EHV-1-XJ2015基因型为非神经型毒株，毒力倾向表现为流产型毒株；遗传进化显示，EHV-1-XJ2015毒株与日本分离株90c16、00c19、HH1、NY03为同一分支，氨基酸同源性最高为100%。本研究结果为开展EHV-1分子流行病学调查研究及为新疆地区现有诊断方法和防控措施的评估提供理论依据。

关键词: EHV-1; ORF30; 致病基因型; 序列比对

Abstract:

To study the genotypes of EHV-1-XJ2015 strain isolated from Ili area of Xinjiang, this study investigated the potential risk factors for EHV-1-XJ2015.In this experiment, specific primers was designed and using PCR technique to amplify corresponding region of EHV-1-XJ2015 isolate ORF30 gene, connected to vector pMD19-T,and the recombinant plasmid pMD19-T-ORF30 was successfully constructed. Sequencing results showed that EHV-1-XJ2015 strain ORF30 gene 2 254 bp site was A, and encoding asparagine (N).It was proved that EHV-1-XJ2015 was a non-neurotoxic strain, virulent strains tend to behave as a miscarriage. The phylogenetic analysis showed that the EHV-1-XJ2015 strain was the same branch as the 90c16, 00c19, HH1, NY03 isolated from Japan, amino acid homology was the highest and reached 100%. The results provided a theoretical basis for the study of EHV-1 molecular epidemiology and the assessment of existing diagnostic methods and prevention and control measures in Xinjiang, and ultimately achieved the purpose of prevention and control of the disease.

Key words: EHV-1; ORF30; pathogenicity genotype; sequence alignment

中图分类号:

S852.65

范斌, 王燕, 阿玛古丽·朱马汉, 冉多良, 刘建华, 加尔肯. 新疆伊犁地区马疱疹病毒1型ORF30基因序列分析[J]. 《中国畜牧兽医》, 2018, 45(1): 65-70.

FAN Bin, WANG Yan, Zhumahan·A, RAN Duoliang, LIU Jianhua, Jarhen. Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang[J]. , 2018, 45(1): 65-70.

参考文献

[1] DAVISON A J, EBERLE R, EHLERS B, et al. The order herpesvirales[J]. Archives of Virology, 2009, 154(1):171.
[2] 杨夷平, 刘建华, 冉多良,等. 马疱疹病毒1型LAMP检测方法的建立[J]. 中国畜牧兽医, 2014, 41(9):35-40. YANG Y P,LIU J H,RAN D L,et al. Establishment of loop-mediated isothermal amplification assay detection method of equine herpesviruses type 1[J]. China Animal Husbandry & Veterinary Medicine,2014, 41(9):35-40.(in Chinese)
[3] WILSON W D. Equine herpesvirus 1 myeloencephalopathy[J]. Veterinary Clinics of North America Equine Practice, 1997, 13(1):53.
[4] CHOWDHURY S I, KUBIN G, LUDWIG H. Equine herpesvirus type 1(EHV-1) induced abortions and paralysis in a Lipizzaner stud:A contribution to the classification of equine herpesviruses[J]. Archives of Virology, 1986, 90(3-4):273.
[5] 阿依吐拉·肉孜, 阿孜古丽, 肖开提,等. 新疆地区马鼻肺炎血清学调查[J]. 中国畜牧兽医文摘, 2013,12:82. AYITULA·ROUZI, AZIGULI,XIAO K T, et al. Serological survey of equine rhinopneumonitis in Xinjiang[J]. Chinese Abstracts of Animal Science and Veterinary Medicine, 2013,12:82.(in Chinese)
[6] 杜润慈, 刘建华, 李静,等. 新疆伊犁部分地区马鼻肺炎血清学调查[J]. 黑龙江畜牧兽医, 2016,22:120-122. DU R C,LIU J H,LI J,et al. Serological survey of equine rhinopneumonitis in parts of Ili prefecture of Xinjiang[J]. Heilongjiang Animal Science and Veterinary Medicine,2016,22:120-122.(in Chinese)
[7] 李娜, 孙建华, 石明,等. 新疆部分地区马鼻肺炎的血清学调查[J]. 动物医学进展, 2014,9:115-117. LI N,SUN J H,SHI M,et al. Serological investigation of equine rhinopneumonitis in part of regions of Xinjiang[J]. Progress in Veterinary Medicine,2014,9:115-117.(in Chinese)
[8] NUGENT J, BIRCHMACHIN I, SMITH K C, et al. Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks[J]. Journal of Virology, 2006, 80(8):4047-4060.
[9] GOODMAN L B, LOREGIAN A, PERKINS G A, et al. A point mutation in a herpesvirus polymerase determines neuropathogenicity[J]. PLoS Pathogens, 2007, 3(11):e160.
[10] GR V D W, GOUPIL R C. A single-nucleotide polymorphism in a herpesvirus DNA polymerase is sufficient to cause lethal neurological disease[J]. Journal of Infectious Diseases, 2009, 200(1):20.
[11] KYDD J H, SMITH K C, HANNANT D, et al. Distribution of equid herpesvirus-1(EHV-1) in respiratory tract associated lymphoid tissue:Implications for cellular immunity[J]. Equine Veterinary Journal, 2010, 26(6):470-473.
[12] GOODMAN L B, WAGNER B, FLAMINIO M J, et al. Comparison of the efficacy of inactivated combination and modified-live virus vaccines against challenge infection with neuropathogenic equine herpesvirus type 1(EHV-1)[J]. Vaccine, 2006, 24(17):3636.
[13] STIERSTORFER B, EICHHORN W, SCHMAHL W, et al. Equine herpesvirus type 1(EHV-1) myeloencephalopathy:A case report[J]. Zoonoses & Public Health, 2002, 49(1):37.
[14] 杨永龙, 刘建华, 宋焕堂,等. 新疆伊犁地区马疱疹病毒1型的分离与鉴定[J]. 中国预防兽医学报, 2016, 38(7):550-553. YANG Y L, LIU J H,SONG H T,et al. Isolation and identification of equine herpesvirus 1 in Xinjiang[J].Chinese Journal of Preventive Veterinary Medicine,2016, 38(7):550-553.(in Chinese)
[15] PUSTERLA N, HUSSEY G S. Equine herpesvirus 1 myeloencephalopathy[J]. Veterinary Clinics of North America Equine Practice, 2014, 30(3):489-506.
[16] CAMPBELL E M, HOPE T J. Gene therapy progress and prospects:Viral trafficking during infection[J]. Gene Therapy, 2005, 12(18):1353-1359.
[17] GOEHRING L S, BRANDES K, WITTENBURG L, et al. Anti-inflammatory drugs will decrease infection of endothelial cell with EHV-1[J]. Journal of Equine Veterinary Science, 2012, 32(10):S63.
[18] ABO-SAKAYA R, EBEID M H, GALILA E S M, et al. D/N752 coding change in DNA polymerase gene (ORF30) plays no role in equid herpesvirus type 1(ehv-1) growth in vitro[J]. Benha Veterinary Medical Journal, 2014, 26(2):1-9.
[19] ALLEN G P, BREATHNACH C C. Quantification by Real-time PCR of the magnitude and duration of leucocyte-associated viraemia in horses infected with neuropathogenic vs. non-neuropathogenic strains of EHV-1[J]. Equine Veterinary Journal, 2006, 38(3):252.
[20] VANDEKERCKHOVE A P, GLORIEUX S, GRYSPEERDT A C, et al. Replication kinetics of neurovirulent versus non-neurovirulent equine herpesvirus type 1 strains in equine nasal mucosal explants[J]. Journal of General Virology, 2010, 91(8):2019-2028.
[21] YAMADA S, MATSUMURA T, TSUJIMURA K, et al. Comparison of the growth kinetics of neuropathogenic and nonneuropathogenicequidherpesvirus type 1(EHV-1) strains in cultured murine neuronal cells and the relevance of the D/N (752) coding change in DNA polymerase gene (ORF30)[J]. Journal of Veterinary Medical Science, 2008, 70(5):505-511.

新疆伊犁地区马疱疹病毒1型ORF30基因序列分析

Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	车传忠, 鲍子磊, 胡月, 郑学功, 刘建华, 冉多良. 马疱疹病毒1型感染马疾病模型的建立及评价[J]. 中国畜牧兽医, 2021, 48(6): 2196-2203.
[2]	范斌, 鲍子磊, 贾钦瑞, 刘建华, 贺笋, 冉多良. 含EGFP基因的马疱疹病毒1型新疆株gE基因缺失株的构建[J]. 中国畜牧兽医, 2020, 47(8): 2652-2659.
[3]	雷程红, 陈凯云, 徐新峰, 胡都斯·艾尔肯, 徐军, 白梅花, 史茜, 肖媛媛, 王科珂. 马疱疹病毒1型QuantStudio^TM 3D数字PCR检测方法的建立[J]. 中国畜牧兽医, 2020, 47(5): 1299-1306.
[4]	范斌, 陈卓, 刘建华, 鲍子磊, 胡月, 贾钦瑞, 王雪竹, 冉多良. 马疱疹病毒1型主要毒力基因分析及TK基因缺失载体的构建[J]. 《中国畜牧兽医》, 2018, 45(10): 2681-2690.
[5]	李思菲, 孙鹏, 陈俊霞, 秦魁, 苏帅, 赵鹏, 崔治中. 一株鸡马立克氏病病毒的分离鉴定及主要致病基因分析[J]. , 2016, 43(5): 1343-1348.
[6]	蒲蕾, 岳静伟, 张金山, 刘欣, 王立刚, 颜华, 侯欣华, 高红梅, 张恬, 张跃博, 武群清, 张龙超, 王立贤. 猪MAP3K5基因CDS序列克隆及生物信息学分析[J]. 《中国畜牧兽医》, 2016, 43(10): 2509-2517.
[7]	程艺，王雨，吉艺宽，孙磊磊，周慧英，琚春梅. 伪狂犬病病毒立即早期180基因的克隆及序列分析[J]. 中国畜牧兽医, 2014, 41(1): 15-18.
[8]	徐超,宋兴超,岳志刚,李光玉,赵蒙,赵伟刚,魏海军,赵家平. 4种犬科动物黑素皮质激素受体1基因序列比对及遗传进化分析[J]. 中国畜牧兽医, 2014, 41(1): 25-29.