牦牛血液氯化血红素的分离提纯及工艺优化研究

doi:10.16431/j.cnki.1671-7236.2017.11.009

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (11): 3163-3169.doi: 10.16431/j.cnki.1671-7236.2017.11.009

牦牛血液氯化血红素的分离提纯及工艺优化研究

杨生忠¹, 韩学燕², 李宗文¹, 陈虎³, 任茜³, 曹效海³

1. 乌兰县畜牧兽医工作站, 乌兰 817100;
2. 大通县畜牧兽医站, 大通 810100;
3. 青海大学农牧学院, 西宁 810016

收稿日期:2017-05-12 出版日期:2017-11-20 发布日期:2017-11-21
通讯作者: 曹效海 E-mail:842700421@qq.com
作者简介:杨生忠(1977-),男,青海西宁人,学士,兽医师,研究方向:动物营养与疫病防治,E-mail:346327749@qq.com
基金资助:
科技厅科技支撑项目"青藏高原动物血液综合利用技术产业化

Study on the Separation and Purification of Hemin from Yak Blood and Its Optimization

YANG Sheng-zhong¹, HAN Xue-yan², LI Zong-wen¹, CHEN Hu³, REN Qian³, CAO Xiao-hai³

1. Animal Husbandry and Veterinary Station in Wulan County, Wulan 817100, China;
2. Animal Husbandry and Veterinary Station in Datong County, Datong 810100, China;
3. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China

Received:2017-05-12 Online:2017-11-20 Published:2017-11-21

摘要/Abstract

摘要：

试验以新鲜牦牛血液为原料，采用血粉法、冰醋酸法及醋酸钠法，探索制备氯化血红素的最优工艺参数，并对不同方法制备的氯化血红素纯度及得量进行对比。结果显示，血粉法的最优工艺是超声处理时间40 min，酸性丙酮添加量为120 mL，抽提时间30 min，乙酸钠添加量为9 mL，氯化血红素纯度达到96%，得量为7.38 g/L。采用冰醋酸法制备氯化血红素，当冰醋酸添加量为红细胞量的3倍时，氯化血红素纯度达到80%，得量为6.10 g/L。采用醋酸钠法制备氯化血红素，当氯仿添加量为红细胞量的2倍时，氯化血红素纯度达到90%，得量为3.44 g/L。通过对比3种制备方法，氯化血红素纯度依次为血粉法 > 醋酸钠法 > 冰醋酸法；氯化血红素得量依次为血粉法 > 冰醋酸法 > 醋酸钠法，由此可知，3种方法中，选用血粉法制备氯化血红素的量更高且更加有利于工业化生产。

关键词: 牦牛血液; 氯化血红素; 牦牛血粉; 醋酸钠法; 冰醋酸法

Abstract:

The study was conducted to separate and purify the hemin from yak blood and the optimal process of preparing the hemin was confirmed using blood meal,glacial acetic acid and sodium acetate methods. The purity and the yield of the extracted hemin in different methods were compared. The results showed that the purity of hemin in yak blood was 96% and the yield was 7.38 g/L using blood meal method under the optimal process of 40 min for ultrasonic treatment, 30 min for extraction,120 mL acid acetone and 9 mL sodium acetate. The purity of hemin was 80% and the yield was 6.10 g/L when the amount of glacial acetic acid was 3 times as much as blood cells amount using the glacial acetic acid method.The purity of hemin was 90% and the yield was 3.44 g/L when the amount of chloroform was 2 times as much as blood cells amount using sodium acetate method. The purity of the hemin from high to low was blood meal method,sodium acetate method and glacial acetic acid method,and that of the hemin yield was blood meal method,glacial acetic acid method and sodium acetate method by comparing the three preparation methods. So the blood meal method was more conducive to industrial production.

Key words: yak blood; hemin; yak blood meal; sodium acetate method; glacial acetic acid method

中图分类号:

S823.8⁺5

杨生忠, 韩学燕, 李宗文, 陈虎, 任茜, 曹效海. 牦牛血液氯化血红素的分离提纯及工艺优化研究[J]. 《中国畜牧兽医》, 2017, 44(11): 3163-3169.

YANG Sheng-zhong, HAN Xue-yan, LI Zong-wen, CHEN Hu, REN Qian, CAO Xiao-hai. Study on the Separation and Purification of Hemin from Yak Blood and Its Optimization[J]. , 2017, 44(11): 3163-3169.

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牦牛血液氯化血红素的分离提纯及工艺优化研究

Study on the Separation and Purification of Hemin from Yak Blood and Its Optimization

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