反向高效液相色谱法检测双峰驼血浆中对乙酰氨基酚的含量

doi:10.16431/j.cnki.1671-7236.2017.08.042

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (8): 2517-2522.doi: 10.16431/j.cnki.1671-7236.2017.08.042

反向高效液相色谱法检测双峰驼血浆中对乙酰氨基酚的含量

散仁¹, 巴拉², 岳伟东¹, 哈斯苏荣^1,3

1. 内蒙古农业大学兽医学院, 农业部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018;
2. 内蒙古阿拉善骆驼科学研究所, 巴彦浩特 750300;
3. 内蒙古骆驼研究院, 巴丹吉林镇 750300

收稿日期:2017-02-12 出版日期:2017-08-20 发布日期:2017-08-18
通讯作者: 哈斯苏荣 E-mail:surong@imau.edu.cn
作者简介:散仁(1989-),女,内蒙古通辽人,硕士生,研究方向:兽医药理学与毒理学,E-mail:ndsarina@126.com
基金资助:
国家自然科学基金项目（31260623）

Determination of Acetaminophen in Bactrian Camel Plasma by RP-HPLC

SAN Ren¹, BA La², YUE Wei-dong¹, HASI Su-rong^1,3

1. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Camel Institute of Alshan, Bayanhot 750300, China;
3. Inner Mongolia Institute of Camel Research, Badanjilin 750300, China

Received:2017-02-12 Online:2017-08-20 Published:2017-08-18

摘要/Abstract

摘要：

试验旨在建立测定双峰驼血浆中对乙酰氨基酚浓度的反相高效液相色谱法（reversed phase high-performance liquid chromatography，RP-HPLC）。选用Supelco Discovery C18色谱柱（150 mm×4.6 mm，5 μm），以甲醇：水=20：80为流动相，流速为0.5 mL/min，紫外检测波长为248 nm，采用甲醇直接沉淀蛋白方法处理血浆样品。结果表明，对乙酰氨基酚的保留时间为6.5 min，分离度良好，无血浆杂峰干扰，且在0.08~10.24 μg/mL浓度范围内线性关系良好（R²=0.9997，n=8），检测限为0.10 μg/mL，日内和日间精密度RSD均小于7.0%，绝对回收率为80.39%~93.56%，相对回收率为94.66%~104.73%。因此，本试验所建立的RP-HPLC法操作简便、准确，重复性好，专属性强，适用于检测双峰驼血浆中对乙酰氨基酚浓度，为后续的借助特异性探针药物——对乙酰氨基酚研究双峰驼CYP1A酶的体内活性提供了可靠而准确的定量方法。

关键词: 双峰驼; 对乙酰氨基酚; RP-HPLC

Abstract:

To establish the reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of plasma concentration of acetaminophen in Bactrian camel, the chromatography was carried on a C18 column (150 mm×4.6 mm,5 μm).The mobile phase was composed of methanol-water (20:80),the flow rate was 0.5 mL/min,and the detection wavelength was set at 248 nm for acetaminophen.The retention time of acetaminophen was 6.5 min, and was not disturbed by other miscellaneous peaks.The calibration curve was linearity in the range of 0.08 to 10.24 μg/mL (R²=0.9997),and the quantitation limit of acetaminophen was 0.10 μg/mL.Both the inter-day and intra-day RSD were under 7.0%, the absolute recovery of the method was 80.39% to 93.56%,the relative recovery of the method was 94.66% to 104.73%.Therefore, the established method was simple, reproducible and accurate,which could be used for determination of plasma acetaminophen concentration and in vivo activities of CYP1A enzyme in Bactrian camel.

Key words: Bactrian camel; acetaminophen; RP-HPLC

中图分类号:

S859.7

散仁, 巴拉, 岳伟东, 哈斯苏荣. 反向高效液相色谱法检测双峰驼血浆中对乙酰氨基酚的含量[J]. 《中国畜牧兽医》, 2017, 44(8): 2517-2522.

SAN Ren, BA La, YUE Wei-dong, HASI Su-rong. Determination of Acetaminophen in Bactrian Camel Plasma by RP-HPLC[J]. , 2017, 44(8): 2517-2522.

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反向高效液相色谱法检测双峰驼血浆中对乙酰氨基酚的含量

Determination of Acetaminophen in Bactrian Camel Plasma by RP-HPLC

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