莱菔硫烷对染镉小鼠睾丸间质细胞抗氧化功能影响的研究

doi:10.16431/j.cnki.1671-7236.2017.02.037

摘要/Abstract

摘要：

试验旨在研究莱菔硫烷（SFN）对染镉（Cd）小鼠睾丸间质细胞（TM3细胞）抗氧化应激能力的影响。向TM3细胞培养液中添加Cd和SFN，分别测定Cd的半数抑制浓度（IC₅₀）和SFN的安全剂量范围，建立试验模型，通过检测TM3细胞的相对存活率、乳酸脱氢酶（LDH）活性及细胞抗氧化水平，判定SFN对Cd诱导TM3细胞毒性的影响。结果显示：①随着Cd浓度的增加，TM3细胞的相对存活率降低，Cd对体外培养TM3细胞的IC₅₀为51.4 μmol/L；SFN在一定范围内对细胞存活有促进作用，但超过范围后具有毒性，且浓度越大毒性越大，本试验条件下选择SFN浓度为2.5、5和10 μmol/L。②与对照组相比，Cd组TM3细胞的T-SOD、GSH-Px活性及GSH含量显著或极显著降低（P < 0.05；P < 0.01），LDH活性、MDA含量升高；SFN组LDH活性、MDA含量降低，GSH含量、T-SOD活性及GSH-Px活力升高；与Cd组相比，Cd＋SFN组GSH含量、T-SOD活性及GSH-Px活力显著或极显著升高（P < 0.05；P < 0.01）；LDH活性、MDA含量则降低，表明SFN具有缓解染Cd诱导TM3细胞毒性的作用。

关键词: 镉; 莱菔硫烷; TM3细胞; 氧化应激

Abstract:

The study was aimed to explore the antioxident function of sulforaphane (SFN) on the leydig cells of cadmium (Cd) exposured mice.Cd and SFN were added to the cell culture medium of TM3 cell, half inhibitory concentration (IC₅₀) of Cd and SFN safe dose range were determined.The tested model was set up,and the relative survival rate of TM3 cells, lactate dehydrogenase (LDH) activity and cell antioxidant levels were determined to study the antioxident function of SFN to cadmium exposured mice.The results showed that:①With the increase of Cd concentration,the relative survival rate of TM3 cells was decreased,and the IC₅₀ of Cd was 51.4 μmol/L;Within a certain concentration range,SFN could increased the cells survive rate,but there was toxicity when the SFN concentration was more than the scope,and the greater the concentration,the greater the toxicity. At experimental condition,SFN safety concentration were 2.5,5 and 10 μmol/L.②Compared with the control group,the GSH content,the T-SOD and GSH-Px activities in Cd group were significantly or extremely significantly decreased (P < 0.05;P < 0.01),and the LDH activity,the MDA content were increased.Additionally,the LDH activity and MDA content of SFN groups were decreased,while others three indexes were increased. Compared with Cd group,the GSH content,the T-SOD and GSH-Px activities of Cd+SFN groups were significantly or extremely significantly increased (P < 0.05;P < 0.01),however,the LDH activity,MDA content were decreased. The result indicated that SFN had the antagonism effect on toxicity of Cd in TM3 cell.

Key words: cadmium; sulforaphane; TM3 cell; oxidative stress

中图分类号:

S858.91

姜丽莹, 何剑斌, 郑伟萍, 张彤, 李慧, 杨淑华. 莱菔硫烷对染镉小鼠睾丸间质细胞抗氧化功能影响的研究[J]. 《中国畜牧兽医》, 2017, 44(2): 568-577.

JIANG Li-ying, HE Jian-bin, ZHENG Wei-ping, ZHANG Tong, LI Hui, YANG Shu-hua. Research on the Antioxident Function of Sulforaphane on the Leydig Cells of Cadmium Exposured Mice[J]. , 2017, 44(2): 568-577.

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