3个马群感染马泰勒虫的分子病原学调查

doi:10.16431/j.cnki.1671-7236.2016.04.035

›› 2016, Vol. 43 ›› Issue (4): 1086-1091.doi: 10.16431/j.cnki.1671-7236.2016.04.035

3个马群感染马泰勒虫的分子病原学调查

薛艳¹, 邓显佑¹, 王嘉福^1,2, 冉雪琴³, 田松军⁴, 魏小红⁴

1. 贵州大学生命科学学院, 贵阳 550025;
2. 贵州大学农业生物工程研究院, 贵阳 550025;
3. 贵州大学动物科学学院, 贵阳 550025;
4. 紫云县畜牧兽医局, 紫云 550800

收稿日期:2015-10-10 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 王嘉福 E-mail:jfwang@gzu.edu.cn
作者简介:薛艳(1987-),女,江苏沛县人,硕士生,研究方向:微生物学,E-mail:992909131@qq.com
基金资助:
贵州省农业攻关项目(黔科合NY字[2012]3009号、黔科合NY字[2009]3068号);贵州省农业动植物育种专项(黔农育专字2009-021号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州大学"本科教学工程"项目(BKJX2012007)

Investigation of Molecular Etiology of Theileria equi Infection in Three Herds

XUE Yan¹, DENG Xian-you¹, WANG Jia-fu^1,2, RAN Xue-qin³, TIAN Song-jun⁴, WEI Xiao-hong⁴

1. Faculty of Life Science, Guizhou University, Guiyang 550025, China;
2. Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China;
3. Faculty of Animal Science, Guizhou University, Guiyang 550025, China;
4. Ziyun Bureau of Animal Husbandry and Veterinary, Ziyun 550800, China

Received:2015-10-10 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 试验采用显微镜观察、PCR和竞争性酶联免疫吸附试验(competitive enzyme-linked immunosorbentassay,cELISA)等方法对贵州矮马、西南马和伊犁马的马泰勒虫病的感染状况进行研究。结果显示,从32份新鲜的血液涂片中,观察到形态完整的马泰勒虫(Theileria equi)虫体,检出率12.5%。从贵州矮马、西南马及伊犁马3个马群血液总DNA中都检测到马泰勒虫的18S rRNA基因片段,与已知序列的同源性为97%~100%;相比之下,贵州矮马与伊犁马的阳性率相近,分别为76.62%和73.81%,西南马较低,仅为33.33%。另外,经cELISA检测,与伊犁马(31.71%)相比,贵州矮马和西南马血液中抗马泰勒虫抗体的阳性率较低,分别为24.68%和12.12%,并与两个马群的血液理化指标存在一定的联系:与中性细胞数量、γ-谷氨酰转移酶和肌酸激酶的含量呈弱正相关;与红细胞、白细胞、血小板、淋巴细胞数量及血红蛋白含量呈弱负相关。这些研究结果提示3个马群中均存在较高比例的马泰勒虫感染。

关键词: 贵州矮马; 西南马; 伊犁马; 马泰勒虫; 抗体; 18S rRNA

Abstract: To explore the prevalence of Theileria equi (T.equi) infection in horse in Guizhou province, the antibody level and 18S rRNA gene were detected from blood samples of Guizhou pony, Southwest horse and Yili horse using competitive ELISA and PCR methods.Giemsa-stained blood smear was prepared to observe T.equi in red blood cells.Intact protozoans of T.equi were observed in red blood cells of horses at Giemsa-stained slide smears with a detection rate of 12.5%.The 18S rRNA gene fragment of T.equi was detected in Guizhou pony, Southwest horse and Yili horse, and the consistent rates with the known nucleotide sequence were 97% to 100%.The PCR result indicated that the positive rates of T.equi in Guizhou pony (76.62%) and Yili horse (73.81%) were similar, which were higher than that in Southwest horse (33.33%).Furthermore, the antibody levels against T.equi in Guizhou pony (24.68%) and Southwest horse (12.12%) were lower than that in Yili horse (31.71%).A weak correlation between the antibody level and the blood physicochemical indexes was calculated from Guizhou pony and Southwest horse, including weak positive correlations with neutrophils numbers, gamma-glutamyl transferase and creatine kinase levels, and weak negative correlations with the numbers of red blood cell, white blood cell, platelet and lymphocyte and contents of hemoglobin.It suggested that a higher proportion of T.equi infection present in three herds.

Key words: Guizhou pony; Southwest horse; Yili horse; Theileria equi; antibody; 18S rRNA

中图分类号:

Q939.9

薛艳, 邓显佑, 王嘉福, 冉雪琴, 田松军, 魏小红. 3个马群感染马泰勒虫的分子病原学调查[J]. , 2016, 43(4): 1086-1091.

XUE Yan, DENG Xian-you, WANG Jia-fu, RAN Xue-qin, TIAN Song-jun, WEI Xiao-hong. Investigation of Molecular Etiology of Theileria equi Infection in Three Herds[J]. , 2016, 43(4): 1086-1091.

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3个马群感染马泰勒虫的分子病原学调查

Investigation of Molecular Etiology of Theileria equi Infection in Three Herds

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