有机农场鸡、猪源大肠杆菌耐药性研究

doi:10.16431/j.cnki.1671-7236.2015.12.035

›› 2015, Vol. 42 ›› Issue (12): 3351-3357.doi: 10.16431/j.cnki.1671-7236.2015.12.035

有机农场鸡、猪源大肠杆菌耐药性研究

植婵萍¹, 郭小婷¹, 姚旭¹, 郭泽文¹, 伍盛鋆¹, 易灵娴¹, 程帮照², 王骏俊², 刘健华¹

1. 华南农业大学兽医学院, 广州 510642;
2. 安徽省宣城市畜牧兽医局, 宣城 242000

收稿日期:2015-05-19 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 刘健华 E-mail:jhliu@scau.edu.cn
作者简介:植婵萍(1990-),女,广东广州人,硕士生,研究方向:兽医药理学与毒理学,E-mail:pingping_zhi@sina.cn 郭小婷(1990-),女,广东河源人,学士,研究方向:动物医学,E-mail:1209278773@qq.com
基金资助:
广东省自然科学基金团队项目(S2012030006590)

Study on Drug Resistance of Escherichia coli Isolated from Chicken and Swine in Organic Farm

ZHI Chan-ping¹, GUO Xiao-ting¹, YAO Xu¹, GUO Ze-wen¹, WU Sheng-jun¹, YI Ling-xian¹, CHENG Bang-zhao², WANG Jun-jun², LIU Jian-hua¹

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Xuancheng Animal Husbandry and Veterinary Bureau of Anhui Province, Xuancheng 242000, China

Received:2015-05-19 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 为了解有机养殖模式下鸡、猪源大肠杆菌的耐药情况,2014年从广东从化某有机农场鸡、猪粪便中采集并分离出118株大肠杆菌,包括55株鸡源大肠杆菌和63株猪源大肠杆菌,采用琼脂稀释法测定大肠杆菌对18种抗菌药的敏感性。结果发现,同一有机农场中,鸡源大肠杆菌与猪源大肠杆菌在耐药种类及耐药率上均呈现相似规律,对头孢他啶、头孢喹肟、头孢噻肟、头孢西丁、阿米卡星、安普霉素、庆大霉素、新霉素、氟苯尼考、氯霉素、亚胺培南、环丙沙星、喹乙醇等较敏感(鸡0~21.8%,猪0~14.3%),对氨苄西林、链霉素呈现轻度耐药(鸡29.1%~38.2%,猪23.8%~27.0%),对复方新诺明、四环素、多西环素呈现较严重耐药(鸡45.5%、76.4%和72.7%,猪50.8%、81.0%和68.3%)。鸡源大肠杆菌中3耐及3耐以上的菌株占56.4%,猪源大肠杆菌占47.6%。结果表明有机养殖模式下鸡、猪源大肠杆菌耐药率相对较低,多重耐药现象存在但相对轻微,对个别临床常用抗菌药耐药率虽较严重但比常规养殖场低。由于目前国内大部分学者致力于研究常规养殖方式下细菌耐药情况,本研究很好地填补了有机养殖模式下畜禽大肠杆菌耐药性研究的空白。

关键词: 有机农场; 大肠杆菌; 耐药性; 多重耐药

Abstract: To determine the drug resistance of E.coli strains from swine and chicken in organic farm,collection of stool specimens was conducted in one organic farm in Conghua city,Guangdong province in 2014,in which 118 E.coli strains were separated,including 55 from chicken and 63 from swine.The sensitivity of the E.coli strains from swine and chicken to 18 kinds of drugs was detected through agar dilution method.Similar properties in drug resistance were observed both in E.coli strains from swine and chicken in the same organic farm.In consideration of the resistance rate,samples were relatively sensitive to ceftazidime,cefquinome,cefoxitin,amikacin,apramycin,gentamicin,neomycin,florfenicol,chloramphenicol,imipenem,ciprofloxacin and olaquindox (chicken 0 to 21.8%;swine 0 to 14.3%).These isolates showed moderate resistance to ampicillin and streptomycin (chicken 29.1% to 38.2%;swine 23.8% to 27.0%).About 56.4% chicken isolates and 47.6% swine isolates showed resistant to more than 3 kinds of drugs.However,severe resistance was observed in trimethoprim-sulphamethoxazole,tetracyclineand and doxycycline (chicken 45.5%,76.4% and 72.7%;swine 50.8%,81.0% and 68.3%).The results also indicated that under organic farming mode,the drug resistance rates of E.coli strains from swine and chicken were relatively low in general.Multi-drug resistance existed but was relatively slight.Although the drug resistance rate to several specific antimicrobials was high,it was still lower than that in conventional farms.Since most studies focused on the drug resistance of the bacteria under conventional farming model,this study filled the blank of the research of drug resistance under organic farming model.

Key words: organic farm; Escherichia coli; drug resistance; multi-drug resistance

中图分类号:

S852.61⁺4

植婵萍, 郭小婷, 姚旭, 郭泽文, 伍盛鋆, 易灵娴, 程帮照, 王骏俊, 刘健华. 有机农场鸡、猪源大肠杆菌耐药性研究[J]. , 2015, 42(12): 3351-3357.

ZHI Chan-ping, GUO Xiao-ting, YAO Xu, GUO Ze-wen, WU Sheng-jun, YI Ling-xian, CHENG Bang-zhao, WANG Jun-jun, LIU Jian-hua. Study on Drug Resistance of Escherichia coli Isolated from Chicken and Swine in Organic Farm[J]. , 2015, 42(12): 3351-3357.

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有机农场鸡、猪源大肠杆菌耐药性研究

Study on Drug Resistance of Escherichia coli Isolated from Chicken and Swine in Organic Farm

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