基于质谱的蛋白质组学技术及其在绒毛用羊中的应用

doi:10.16431/j.cnki.1671-7236.2015.12.011

›› 2015, Vol. 42 ›› Issue (12): 3179-3185.doi: 10.16431/j.cnki.1671-7236.2015.12.011

基于质谱的蛋白质组学技术及其在绒毛用羊中的应用

王乐乐¹, 赵濛¹, 韩文静¹, 王志新¹, 张燕军¹, 李金泉^1,2

1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 内蒙古自治区动物遗传育种与繁殖重点实验室, 呼和浩特 010018

收稿日期:2015-04-24 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 张燕军, 李金泉 E-mail:imauzyj@126.com;lijinquan_nd@126.com
作者简介:王乐乐(1985-),女,河南驻马店人,博士生,研究方向:动物遗传育种与繁殖,E-mail:leleruwu@sina.cn
基金资助:
国家高技术研究发展计划项目(863计划)( 2013AA100506);国家农业部现代农业产业技术体系项目(CARS-40-05);内蒙古农业大学优秀青年科学基金(2014XYQ-1)

Proteomics Technologies based on Mass Spectrometry and their Application in Wool-sheep and Cashmere-goat

WANG Le-le¹, ZHAO Meng¹, HAN Wen-jing¹, WANG Zhi-xin¹, ZHANG Yan-jun¹, LI Jin-quan^1,2

1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot 010018, China

Received:2015-04-24 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 蛋白质组学(proteomics)是指应用蛋白质分离、鉴定和定量的技术研究蛋白质组的一门学科,与之对应的蛋白质组学技术随之快速发展,基于质谱的蛋白质组学技术更是在相关领域广泛应用。作者主要就蛋白质的相互作用、差异蛋白质组学和蛋白质的定量研究,一些基于质谱的蛋白质组学技术的原理和优缺点,以及绒毛用羊毛囊研究现状和基于质谱的蛋白质组学技术在绒毛用羊中的应用进展等方面进行了综述。最后,对蛋白质组学技术在绒毛用羊毛囊研究中的应用前景做出展望。

关键词: 蛋白质组学技术; 质谱; 绒毛用羊

Abstract: Proteomics is a discipline which study proteome using the technology of protein isolation,identification and quantitative.Proteomics technology also has greatly developed,and proteomics technologies based on mass spectrometry have been widely used in the related field.In this review,we mainly introduced the advantages and disadvantages of some proteomic technologies based on mass spectrometry on the studies of protein-protein interactions,differential proteomics and quantitative proteomics,reviewed the advance of hair follicles and focused on the proteomic technologies application in wool-sheep and cashmere-goat.Finally,some prospects of proteomics technologies application in the hair follicles study of wool-sheep and cashmere-goat were presented.

Key words: proteomics technologies; mass spectrometry; wool-sheep and cashmere-goat

中图分类号:

王乐乐, 赵濛, 韩文静, 王志新, 张燕军, 李金泉. 基于质谱的蛋白质组学技术及其在绒毛用羊中的应用[J]. , 2015, 42(12): 3179-3185.

WANG Le-le, ZHAO Meng, HAN Wen-jing, WANG Zhi-xin, ZHANG Yan-jun, LI Jin-quan. Proteomics Technologies based on Mass Spectrometry and their Application in Wool-sheep and Cashmere-goat[J]. , 2015, 42(12): 3179-3185.

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基于质谱的蛋白质组学技术及其在绒毛用羊中的应用

Proteomics Technologies based on Mass Spectrometry and their Application in Wool-sheep and Cashmere-goat

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