褐黄血蜱雌蜱不同消化阶段中肠蛋白组学分析

doi:10.16431/j.cnki.1671-7236.2021.02.023

摘要/Abstract

摘要： 本研究旨在探明4个时间段褐黄血蜱（采自刺猬体表）中肠蛋白质成分，揭示参与血餐消化的蛋白种类及其含量变化规律。采用液相色谱-串联质谱法（LC-MS/MS）对采集于刺猬体表的4个时间段褐黄血蜱的中肠蛋白组成分进行检测。基于该蜱的唾液、中肠转录组翻译文库及Uniprot数据库，利用软件Mascot 2.2对所获肽段及蛋白质进行鉴定。结果显示：在褐黄血蜱中肠蛋白提取液中共检测出特异性肽段3 046条，鉴定303种蛋白，其中271种为高可信蛋白；在所有的高可信蛋白中，23种含量较为丰富，125种在后期消化阶段（第2至第4阶段）的含量为0，123种的含量发生不同程度的变化（24种蛋白的含量变化明显，其中12种含量上升明显，12种含量下降明显）。确定148种高可信蛋白来自于刺猬血清，推断24种可信蛋白可能参与蜱虫对血餐的消化。

关键词: 液相色谱-串联质谱; 褐黄血蜱; 中肠蛋白

Abstract: The aim of this study was to explore the protein components of midgut contents of adult female haemaphysalis flava collected from hedgehog body surface at four different stages and reveal the types and content changes of protein involved in blood meal digestion.The midgut contents from Haemaphysalis flava at four different stages were detected by LC-MS/MS,and the peptides and proteins obtained were identified and analyzed by using the computer software Mascot 2.2 based on previous established salivary and midgut transcriptome translation library and UniProt database.The results of this study showed that a total of 3 046 unique peptides and 303 proteins were detected and identified.Total of 271 proteins with high credible among 303 proteins identified.Among 271 high credible proteins,23 proteins had abundant contents at first stage,125 types of proteins had zero contents at later stages (from second stage to fourth stage),123 types proteins' contents changed in different degrees.Among 123 proteins with contents changed,there were 24 proteins with contents obvious change,which include 12 proteins that their contents rose obviously and 12 proteins that their contents descended obviously at four stages.It was determined that 148 high credible proteins came from hedgehog serum,and in which 24 high credible proteins may be involved in ticks digestion blood meal.

Key words: LC-MS/MS; Haemaphysalis flava; midgut proteins

中图分类号:

李中波, 尧国民, 侯强红, 程天印. 褐黄血蜱雌蜱不同消化阶段中肠蛋白组学分析[J]. 中国畜牧兽医, 2021, 48(2): 607-618.

LI Zhongbo, YAO Guomin, HOU Qianghong, CHENG Tianyin. Proteomic Profiling of the Midgut Contents of Female Haemaphysalis flava at Different Digestion Stage[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 607-618.

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