大白猪肿瘤坏死因子α基因启动子区多态性及其对mRNA表达水平的影响

doi:10.16431/j.cnki.1671-7236.2015.06.029

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (6): 1518-1523.doi: 10.16431/j.cnki.1671-7236.2015.06.029

大白猪肿瘤坏死因子α基因启动子区多态性及其对mRNA表达水平的影响

孙丽, 夏日炜, 刘颖, 孙寿永, 包文斌, 吴圣龙

扬州大学动物科学与技术学院, 江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009

收稿日期:2014-11-03 出版日期:2015-06-20 发布日期:2015-07-23
通讯作者: 吴圣龙 E-mail:slwu@yzu.edu.cn
作者简介:孙丽(1992-),女,江苏如皋人,硕士生,研究方向:猪遗传育种,E-mail:sl19920327@163.com
基金资助:
江苏省科技支撑计划(BE2013319、BE2014328);常州市科技支撑计划(CE2013011)

Polymorphism of TNF-α Gene Promoter and its Effect on mRNA Expression in Large White Pigs

SUN Li, XIA Ri-wei, LIU Ying, SUN Shou-yong, BAO Wen-bin, WU Sheng-long

Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2014-11-03 Online:2015-06-20 Published:2015-07-23

摘要/Abstract

摘要： 肿瘤坏死因子α(tumor necrosis factor,TNF-α)基因在机体固有免疫和炎症反应中发挥重要作用,本研究旨在分析大白猪TNF-α基因启动子区C-791T多态性及其与基因表达之间的关系,为分析TNF-α基因启动子区C-791T突变的遗传学效应提供科学依据.本试验利用PCR-SSCP方法对201头大白猪TNF-α基因启动子区C-791T位点多态性进行检测,同时利用Real-time PCR方法分析TNF-α基因C-791T位点不同基因型在大白猪11个组织中的mRNA差异表达情况.结果表明,大白猪TNF-α基因启动子区-791 bp处存在碱基突变(C> T),并检测到3种基因型,分别为:CC、CT和TT.TNF-α基因在断奶仔猪脾脏、肺脏、胸腺和淋巴结等免疫器官中表达量均较高;TT和CT基因型个体在11个组织中TNF-α基因表达量普遍高于CC基因型个体,且在十二指肠及空肠组织中达到差异显著性水平(P <0.05).因此,TNF-α基因启动子区C-791T突变对该基因的mRNA表达水平产生显著影响,有必要将TNF-α基因启动子区C-791T位点多态性作为提升机体肠道病原菌抗性的遗传标记进行深入研究.

关键词: 猪; TNF-α 基因; 多态性; 差异表达

Abstract: Tumor necrosis factor α (TNF-α) gene plays an important role in innate immunity and inflammatory responses,this study aimed at analyzing the relationship between the polymorphism of TNF-α gene promoter and gene expression in Large White pigs,and further providing a scientific basis for analyzing the genetic effect of TNF-α gene promoter C-791T mutation.This study detected the polymorphism at C-791T site of TNF-α gene promoter in 201 heads of Large White pigs by PCR-SSCP method.Meanwhile,the expression of TNF-α gene in 11 tissues was assayed by Real-time PCR and analyzed the differential expression among individuals with different genotypes at C-791T site.The results showed that there existed one mutation (C-791T) in TNF-α gene promoter and three genotypes were detected,which were CC, CT and TT.The mRNA expressions of TNF-α were higher in spleen,lung,thymus and lymph of weaned piglets.TNF-α gene expression levels of TT and CT genotypes individual were generally higher than CC genotype individual in 11 tissues,and the mRNA expression in TT and CT individuals were significant higher than that in CC individuals in the duodenum and jejunum tissue (P <0.05).Therefore,the C-791T mutation of TNF-α gene promoter indeed had a significant influence on the mRNA expression level of TNF-α gene,which indicated that it was necessary for us to carry out an in depth study of TNF-α gene promoter C-791T polymorphism as genetic markers of enhancing the resistance to intestinal pathogenic bacteria.

Key words: pig; TNF-α gene; polymorphism; differential expression

中图分类号:

S813.3

孙丽, 夏日炜, 刘颖, 孙寿永, 包文斌, 吴圣龙. 大白猪肿瘤坏死因子α基因启动子区多态性及其对mRNA表达水平的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(6): 1518-1523.

SUN Li, XIA Ri-wei, LIU Ying, SUN Shou-yong, BAO Wen-bin, WU Sheng-long. Polymorphism of TNF-α Gene Promoter and its Effect on mRNA Expression in Large White Pigs[J]. , 2015, 42(6): 1518-1523.

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大白猪肿瘤坏死因子α基因启动子区多态性及其对mRNA表达水平的影响

Polymorphism of TNF-α Gene Promoter and its Effect on mRNA Expression in Large White Pigs

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