Pax7基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律研究

doi:10.16431/j.cnki.1671-7236.2015.06.027

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (6): 1504-1511.doi: 10.16431/j.cnki.1671-7236.2015.06.027

Pax7基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律研究

牛姣艳¹, 高鹏飞¹, 马丽¹, 刘翠翠¹, 王泽艺¹, 杨青春¹, 浦忠得¹, 张旗¹, 刘宏², 郭晓红¹, 李步高¹, 曹果清¹

1. 山西农业大学动物科技学院, 太谷 030801;
2. 大同市种猪场, 大同 037000

修回日期:2015-03-26 出版日期:2015-06-20 发布日期:2015-07-23
通讯作者: 曹果清 E-mail:anniecao710502@aliyun.com
作者简介:牛姣艳(1988-),女,山西吕梁人,硕士生,研究方向:分子遗传学与动物育种,E-mail:niujiaoyan88@126.com 高鹏飞(1980-),男,山西太谷人,博士,副教授,研究方向:动物遗传育种与繁殖,E-mail:gpf800411@126.com
基金资助:
山西省科技攻关项目(20140311020-5、20080311031);山西省留学人员科技活动择优资助项目(614193);山西省高等学校优秀青年学术带头人支持计划;山西农业大学"中青年学术带头人及学术骨干"支持计划

Study on the Expression Profile of Pax7 Gene and its Developmental Expression in Longissimus Dorsi of Pigs

NIU Jiao-yan¹, GAO Peng-fei¹, MA Li¹, LIU Cui-cui¹, WANG Ze-yi¹, YANG Qing-chun¹, PU Zhong-de¹, ZHANG Qi¹, LIU Hong², GUO Xiao-hong¹, LI Bu-gao¹, CAO Guo-qing¹

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. Datong Pig Breeding Farm, Datong 037000, China

Revised:2015-03-26 Online:2015-06-20 Published:2015-07-23

摘要/Abstract

摘要： 本试验旨在探究Pax7(paired box 7)基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律.采用实时荧光定量PCR(qRT-PCR)技术和Western blotting技术检测了Pax7基因在1日龄马身猪心脏、肝脏、脾脏、肺脏、肾脏、胃、小肠、下丘脑、小脑、背最长肌、腰大肌、股二头肌12种组织中的mRNA和蛋白质表达谱,以及马身猪和大白猪从出生1日龄到180日龄7个发育阶段(1、30、60、90、120、150、180日龄)背最长肌中的发育性表达规律.结果表明,Pax7基因mRNA在背最长肌、腰大肌、股二头肌、下丘脑和小脑组织中表达,而Pax7蛋白仅在背最长肌、腰大肌和股二头肌中表达.背最长肌中Pax7基因mRNA和蛋白质的发育性表达趋势在马身猪和大白猪中基本一致.在mRNA水平上,30日龄的表达量最高,极显著高于其他日龄(P <0.01);1日龄的表达量次之;其他日龄维持低表达的稳定状态.Pax7蛋白表达量在1日龄时最高,极显著高于其他日龄(P <0.01);30日龄次之;其他日龄维持低表达的稳定状态.从出生1日龄到180日龄,马身猪背最长肌中Pax7蛋白的表达量均显著或极显著地高于大白猪(P <0.05;P <0.01).Pax7 基因的表达与组织、日龄及品种的遗传背景有关.

关键词: 猪; Pax7基因; 表达谱; 发育性表达

Abstract: The aim of this study was to investigate the expression profile of Pax7 (paired box 7) gene in different tissues and its developmental expression in longissimus dorsi of pigs.The expression profiles of Pax7 gene at the levels of mRNA and protein in 12 different tissues including heart,liver,spleen,lung,kidney,stomach,small intestine,hypothalamus,cerebellum,longissimus dorsi,biceeps femoris,psoas major of Mashen pigs at birth and the developmental expression patterns in longissimus dorsi at seven stages (1,30,60,90,120,150,and 180-day old) of Mashen and Large White pigs were studied by quantitative Real-time PCR and Western blotting.The results showed that the Pax7 mRNA was expressed in longissimus dorsi,biceeps femoris,psoas major,hypothalamus,and cerebellum,wherease the Pax7 protein was only expressed in skeletal muscles of longissimus dorsi,biceeps femoris,and psoas major.The developmental expression patterns of Pax7 mRNA and protein in Mashen pig was basically in accordance with those in Large White pig.The mRNA expression amount of Pax7 gene in longissimus dorsi was the greatest at 30-day old (P <0.01),followed by the expression at 1-day old,and kept lower stable levels at other stages both in Mashen and Large White pigs.At the stages of 30-day,90-day,and 180-day old,Pax7 mRNA expression amounts in Mashen pig were significantly higher than those in Large White pig (P <0.01).The Pax7 protein expression at 1-day old was the greatest (P <0.01),followed by it at 30-day old,which were significantly greater than those at other stages,at which Pax7 expression was in lower stable levels.The Pax7 protein expression from one-day old to 180-day old in Mashen pig was significantly greater than those in Large White pig (P <0.05;P <0.01).The expression of Pax7 gene could associated with tissue,age,and background of pig breed.

Key words: pig; Pax7 gene; expression profile; developmental expression

中图分类号:

S828.2

牛姣艳, 高鹏飞, 马丽, 刘翠翠, 王泽艺, 杨青春, 浦忠得, 张旗, 刘宏, 郭晓红, 李步高, 曹果清. Pax7基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律研究[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(6): 1504-1511.

NIU Jiao-yan, GAO Peng-fei, MA Li, LIU Cui-cui, WANG Ze-yi, YANG Qing-chun, PU Zhong-de, ZHANG Qi, LIU Hong, GUO Xiao-hong, LI Bu-gao, CAO Guo-qing. Study on the Expression Profile of Pax7 Gene and its Developmental Expression in Longissimus Dorsi of Pigs[J]. , 2015, 42(6): 1504-1511.

参考文献

[1] Lang D,Powell S K,Plummer R S,et al.PAX genes:Roles in development,pathophysiology,and cancer[J].Biochem Pharmacol,2007,73(1):10-14.

[2] Jostes B,Walther C,Gruss P.The murine paired box gene,Pax7,is expressed specifically during the development of the nervous and muscular system[J].Mech Develop,1990,33(1):27-37.

[3] Peters H,Wilm B,Sakai N,et al.Pax1 and Pax9 synergistically regulate vertebral column development[J]. Development,1999,126(23):5399-5408.

[4] Ziman M R,Thomas M,Jacobsen P,et al.A key role for Pax7 transcripts in determination of muscle and nerve cells[J].Exp Cell Res,2001,268(2):220-229.

[5] Mansouri A,Gruss P.Pax3 and Pax7 are expressed in commissural neurons and restrict ventral neuronal identity in the spinal cord[J]. Mech Develop,1998,78(1):171-178.

[6] Cai L,Taylor J F,Wing R A,et al.Construction and characterization of a bovine bacterial artificial chromosome library[J]. Genomics,1995,29(2):413-425.

[7] Sibthorpe D,Sturlaugsdottir R,Kristjansson B K,et al.Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr (Salvelinus alpinus)[J]. Comp Biochem Phys B,2006,145(3-4):371-383.

[8] James E N M,Simon M H.Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest[J]. Dev Biol,2008,317(2):508-522.

[9] Thomas M G,Barker R A,Beazley L D,et al.Pax7 expression in the adult rat superior colliculus following optic nerve injury[J]. Neuroreport,2007,18(2):105-109.

[10] Hyatt J P K,McCall G E,Kander E M,et al.PAX3/7 expression coincides with MyoD during chronic skeletal muscle overload[J].Muscle Nerve,2008,38(1):861-866.

[11] Seger C,Hargrave M,Wang X,et al.Analysis of Pax7 expressing myogenic cells in zebrafish muscle development,injury,and models of disease[J].Dev Dynam,2011,240(11):2440-2451.

[12] Bhagavati S,Song X,Siddiqui M A Q.RNAi inhibition of Pax3/7 expression leads to markedly decreased expression of muscle determination genes[J].Mol Cell Biochem,2007,302(1-2):257-262.

[13] Galli L M,Knight S R,Barnes T L,et al.Identification and characterization of subpopulations of Pax3 and Pax7 expressing cells in developing chick somites and limb buds[J]. Dev Dynam,2008,237(7):1862-1874.

[14] Seale P,Rudnicki M A.A new look at the origin,function,and "stem-cell" status of musclesatellite cells[J].Dev Biol,2000,218(2):115-124.

[15] Buckingham M,Rigby P W J.Gene regulatory networks and transcriptional mechanisms that control myogenesis[J].Dev Cell, 2014,28(3):225-238.

[16] Maltzahn J V,Jones A E,Parks R J,et al.Pax7 is critical for the normal function of satellite cells in adult skeletal muscle[J].Proc Natl Acad Sci USA,2013,110(41):14674-14679.

[17] 郑江,邓忠良.Pax7对肌肉形成作用的研究进展[J].重庆医科大学学报,2007,32(6):667-669.

[18] Oustanina S,Hause G,Braun T.Pax7 directs postnatal renewal and propagation of myogenic satellite cells but not their specification[J].Embo,2004,23(16):3430-3439.

[19] Gotensparre S M,Andersson E,Wargelius A,et al.Insight into the complex genetic network of tetraploid Atlantic salmon (Salmo salar L.):Description of multiple novel Pax-7 splice variants[J]. Gene,2006,373:8-15.

[20] 甘甜,冷向军,郭婷,等.草鱼Pax7基因的克隆、序列分析及组织表达[J].生物技术通报,2014,11:152-158.

[21] 陈禧.鸭Pax3/7 cDNA序列克隆及表达特性研究[D].雅安:四川农业大学,2012.

[22] Chen J,Wang D Z.microRNAs in cardiovascular development[J].Mol Cell Cardiol,2012,52:949-957.

[23] 吕萍,马景涛.骨骼肌卫星细胞的分离及培养[J].河北医科大学学报,2006,27(4):305-307.

[24] 甘乾福.牛肌肉生长和脂肪代谢相关转录因子的克隆、表达及遗传效应分析[D].杨凌:西北农林科技大学,2009.

[25] Patruno M,Caliaro F,Maccatrozzo L,et al.Myostatin shows a specific expression pattern in pig skeletal and extraocular muscles during pre and post-natal growth[J].Differention,2008,76(2):168-181.

[26] Ropka M K,Eckert R,Piorkowska K.The expression pattern of myogenic regulatory factors MyoD,Myf6 and Pax7 in postnatal porcine skeletal muscles[J]. Gene Expr Patterns,2011,11(1-2):79-83.

[27] Yin H D,Li D Y,Zhang M Y,et al.Housing system influences abundance of Pax3 and Pax7 in postnatal chicken skeletal muscles[J].Poult Sci,2014,93(6):1337-1343.

[28] Qin W,Bruce M P.Regulation of MyoD function in the dividing myoblast[J].Febs Lett,2001,490(3):171-178.

[29] 赵晓,莫德林,张悦,等.猪的骨骼肌生长发育研究进展[J].生命科学,2011,23(1):37-44.

[30] Zhao P,Hofman E P.Embryonic myogenesis pathways in muscle regeneration[J].Dev Dyn,2004,229(2):380-392.

[31] 贾静敏.马身猪和大白猪肌球蛋白重链(MyHC)的发育性变化及肌纤维组织学观察[D].太谷:山西农业大学,2012.

Pax7基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律研究

Study on the Expression Profile of Pax7 Gene and its Developmental Expression in Longissimus Dorsi of Pigs

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