犊牛组织中小肽转运体(POTs)的表达研究

doi:10.16431/j.cnki.1671-7236.2015.05.022

›› 2015, Vol. 42 ›› Issue (5): 1180-1186.doi: 10.16431/j.cnki.1671-7236.2015.05.022

犊牛组织中小肽转运体(POTs)的表达研究

郭静, 杨宏波, 陈银岳, 刘红, 赵国琦

扬州大学动物科学与技术学院, 扬州 225009

收稿日期:2014-10-29 出版日期:2015-05-20 发布日期:2015-06-02
通讯作者: 赵国琦 E-mail:jszhaoguoqi@sohu.com
作者简介:郭静(1989-),女,江苏扬州人,硕士生,研究方向:动物营养与饲料科学,E-mail:yzguojing@hotmail.com
基金资助:
国家"十二五"科技支撑计划项目(2011BAD17B02);国家科技支撑项目(2012BAD34B04);江苏省科技支撑项目(BE2013387)

Expression of Proton-dependent Oligopeptide Transporters (POTs) in Calves' Tissues

GUO Jing, YANG Hong-bo, CHEN Yin-yue, LIU Hong, ZHAO Guo-qi

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2014-10-29 Online:2015-05-20 Published:2015-06-02

摘要/Abstract

摘要： 为研究犊牛部分组织中小肽转运体(proton-dependent oligopeptide transporters,POTs) (PepT1、PepT2、PHT1、PHT2) mRNA的表达,试验选用4头3月龄的中国荷斯坦犊牛进行屠宰,采用实时荧光定量PCR方法来定量组织中小肽转运蛋白表达水平。结果表明,犊牛PepT1 mRNA在瘤胃中表达丰度极显著高于心脏和肌肉(P<0.01);犊牛PepT2 mRNA在肝脏和肾脏中表达丰度极显著高于心脏、脾脏、胸腺、肌肉、瘤胃、网胃、瓣胃、皱胃(P<0.01);犊牛PHT1 mRNA在肺脏和脾脏中表达丰度极显著高于心脏和肌肉(P<0.01);犊牛PHT2 mRNA在肺脏和胸腺中表达丰度显著高于心脏、肝脏、肾脏、脾脏、肌肉、瘤胃、网胃、瓣胃、皱胃(P<0.05)。综上所述,PepT1、PepT2、PHT1、PHT2 mRNA分别在犊牛瘤胃、肾脏和肝脏、脾脏和肺脏、肺脏和胸腺中表达量最高。

关键词: PepT1; PepT2; PHT1; PHT2; 犊牛

Abstract: This test was designed to study the expression of POTs (PepT1, PepT2, PHT1 and PHT2) mRNA in calves'tissues.Tissue-specific expression of the mRNA corresponding to peptide transporter protein in four 3-month-old Chinese Holstein calves was examined by relative quantitative RT-PCR analysis.The results showed that the expression of PepT1 mRNA in the rumen was extremely significantly higher than that in the heart and muscle (P<0.01);The expression of PepT2 mRNA in the liver and kidney was extremely significantly higher than that in the heart, spleen, thymus, muscle, rumen, reticulum, omasum and abomasum (P<0.01);The expression of PHT1 mRNA in the lung and spleen was extremely significantly higher than that in the heart and muscle (P<0.01);The expression of PHT2 mRNA in the lung and thymus was significantly higher than that in the heart, liver, kidney, spleen, muscle, rumen, reticulum, omasum and abomasum (P<0.05).The results indicated that PepT1, PepT2, PHT1 and PHT2 mRNA were respectively abundantly expressed in rumen, kidney and liver, spleen and lung, lung and thymus.

Key words: PepT1; PepT2; PHT1; PHT2; calves

中图分类号:

S823

郭静, 杨宏波, 陈银岳, 刘红, 赵国琦. 犊牛组织中小肽转运体(POTs)的表达研究[J]. , 2015, 42(5): 1180-1186.

GUO Jing, YANG Hong-bo, CHEN Yin-yue, LIU Hong, ZHAO Guo-qi. Expression of Proton-dependent Oligopeptide Transporters (POTs) in Calves' Tissues[J]. , 2015, 42(5): 1180-1186.

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犊牛组织中小肽转运体(POTs)的表达研究

Expression of Proton-dependent Oligopeptide Transporters (POTs) in Calves' Tissues

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