水貂、狐狸和貉源犬瘟热病毒F蛋白信号肽区基因克隆及遗传变异分析

doi:10.16431/j.cnki.1671-7236.2015.04.010

›› 2015, Vol. 42 ›› Issue (4): 838-846.doi: 10.16431/j.cnki.1671-7236.2015.04.010

水貂、狐狸和貉源犬瘟热病毒F蛋白信号肽区基因克隆及遗传变异分析

孙彦刚¹, 赵建军¹, 白雪¹, 牛登云², 邵西群¹, 胡博¹, 刘昊¹, 张海玲¹, 张蕾¹, 王洋¹, 马凡舒¹, 闫喜军¹

1. 中国农业科学院特产研究所经济动物疫病研究室, 长春 130122;
2. 青岛农业大学动物科技学院, 青岛 266109

收稿日期:2014-11-14 出版日期:2015-04-20 发布日期:2015-05-05
通讯作者: 闫喜军 E-mail:tcsyxj@126.com
作者简介:孙彦刚(1989-),男,河南安阳人,硕士,研究方向:动物传染病及其病原分子流行病学,E-mail:wedeyouxiang1989@126.com
基金资助:
吉林省科技发展计划青年科研基金项目(20130522089JH);重点科技成果转化项目(20140307002NY)

Cloning and Genetic Variability Analysis of Single Peptide Gene Regions of Fusion Protein of Canine Distemper Virus from Minks,Foxes and Raccoon Dogs

SUN Yan-gang¹, ZHAO Jian-jun¹, BAI Xue¹, NIU Deng-yun², SHAO Xi-qun¹, HU Bo¹, LIU Hao¹, ZHANG Hai-ling¹, ZHANG Lei¹, WANG Yang¹, MA Fan-shu¹, YAN Xi-jun¹

1. Division of Economic Animal Epidemic, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130122, China;
2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China

Received:2014-11-14 Online:2015-04-20 Published:2015-05-05

摘要/Abstract

摘要： 为了解近年来犬瘟热病毒(canine distemper virus,CDV)在中国毛皮动物主要养殖区的流行情况及遗传变异情况,本试验于2012-2014年从山东、河北、辽宁收集疑似犬瘟热的水貂、狐狸和貉病料,经犬瘟热抗原检测试纸条检测和RT-PCR检测为阳性后,克隆测序了15株CDV F蛋白信号区(Fsp)基因序列.序列分析结果显示,15株CDV野毒株Fsp基因核苷酸序列和氨基酸序列的相似性均为93.6%~100.0%,与疫苗株相似性为80.7%~81.7%.基因系统进化分析结果显示,15株野毒株均属于中国当前流行的Asia-1基因型.氨基酸序列比对分析结果显示,Fsp蛋白在氨基酸3-14、16-37、47-67位等处存在高变异.通过对当前流行CDV野毒株Fsp基因序列分析,结果表明该Fsp基因区具有较高的变异率,可作为CDV基因分型的依据,同时本试验结果为毛皮动物犬瘟热的防控提供了参考依据.

关键词: 水貂; 狐狸; 貉; 犬瘟热病毒; F蛋白信号肽(Fsp)

Abstract: To investigate the prevalence and genetic diversity of canine distemper virus (CDV) in the main breeding areas of fur animal in China in recent years, the tissue samples of the minks, foxes and raccoon dogs with symptoms of canine distemper (CD) were collected from Shandong, Hebei and Liaoning provinces in the years 2012 to 2014.The signal peptide of fusion protein (Fsp) genes of 15 CDV wild-type strains were cloned and sequenced after the samples were identified to be positive of CD by one step CDV rapid test kit and RT-PCR. The results showed that the identities of nucleotide and amino acid sequences of the 15 CDV wild-type strains were 93.6% to 100.0%, while the identities of nucleotide and amino acid sequences of the isolates and vaccines were 80.7% to 81.7%. The phylogenetic analysis of Fsp genes revealed the 15 CDV wild-type strains were characterized as an Asia-1 genotype that were circulating currently in China. The alignment analysis of amino acid sequences indicated there were obviously diversity at the amino acid sites 3 to 14, 16 to 37, 47 to 67.The sequences analysis of Fsp gene of the currently circulating CDV wild-type strains suggested this Fsp gene region had high divergence and could be used to identify the CDV genotypes that would provide foundation for the prevention and control of CD in the fur animal.

Key words: mink; fox; raccoon dog; canine distemper virus; signal peptide of fusion protein (Fsp)

中图分类号:

S852.65

孙彦刚, 赵建军, 白雪, 牛登云, 邵西群, 胡博, 刘昊, 张海玲, 张蕾, 王洋, 马凡舒, 闫喜军. 水貂、狐狸和貉源犬瘟热病毒F蛋白信号肽区基因克隆及遗传变异分析[J]. , 2015, 42(4): 838-846.

SUN Yan-gang, ZHAO Jian-jun, BAI Xue, NIU Deng-yun, SHAO Xi-qun, HU Bo, LIU Hao, ZHANG Hai-ling, ZHANG Lei, WANG Yang, MA Fan-shu, YAN Xi-jun. Cloning and Genetic Variability Analysis of Single Peptide Gene Regions of Fusion Protein of Canine Distemper Virus from Minks,Foxes and Raccoon Dogs[J]. , 2015, 42(4): 838-846.

参考文献

[1] Kauffman C A,Bergman A G,Óconnor R P.Distemper virus infection in ferrets:An animal model of measles-induced immunosuppression[J].Clin Exp Immunol,1982,47:617-625.
[2] Beineke A,Puff C,Seehusen F,et al.Pathogenesis and immunopathology of systemic and nervous canine distemper[J].Vet Immunol Immunopathol,2009,127(1-2):1-18.
[3] Carvalho O V,Botelho C V,Ferreira C G T,et al.Immunopathogenic and neurological mechanisms of canine distemper virus[J].Adv Virol,2012,2012:163860.
[4] Seimon T A,Miquelle D G,Chang T Y,et al.Canine distemper virus:An emerging disease in wild endangered Amur tigers (Panthera tigris altaica)[J].mBio,2013,4(4):e00410-e00413.
[5] Di Sabatino D,Lorusso A,Di Francesco C E,et al.Arctic lineage-canine distemper virus as a cause of death in Apennine wolves (Canis lupus) in Italy[J]. PLoS One,2014,9(1):e82356.
[6] Meli M L,Simmler P,Cattori V,et al.Importance of canine distemper virus (CDV) infection in free-ranging Iberian lynxes (Lynx pardinus)[J].Vet Microbiol,2010,146(1-2):132-137.
[7] Cottrell W O,Keel M K,Brooks J W,et al.First report of clinical disease associated with canine distemper virus infection in a wild black bear (Ursus americana)[J]. J Wildl Dis,2013,49(4):1024-1027.
[8] Blixenkrone-moller M,Svansson V,Have P,et al.Studies on manifestations of canine distemper virus infection in an urban dog population[J].Vet Microbiol,1993,37(1-2):163-173.
[9] von Messling V,Zimmer G,Herrler G,et al.The hemagglutinin of canine distemper virus determines tropism and cytopathogenicity[J].J Virol,2001,75(14):6418-6427.
[10] Hashimoto M,Une Y,Mochizuki M.Hemagglutinin genotype profiles of canine distemper virus from domestic dogs in Japan[J].Arch Virol,2001,146:149-155.
[11] von Messling V,Cattaneo R.Amino-terminal precursor sequence modulates canine distemper virus fusion protein function[J].J Virol,2002,76(9):4172-4180.
[12] Lee M S,Tsai K J,Chen L H,et al.The identification of frequent variations in the fusion protein of canine distemper virus[J].Vet J,2010,183(2):184-190.
[13] Sarute N,Calderón M G,Pérez R,et al.The fusion protein signal-peptide-coding region of canine distemper virus:A useful tool for phylogenetic reconstruction and lineage identification[J].PLoS One,2013,8(5):e63595.
[14] Sarute N,Perez R,Aldaz J,et al.Molecular typing of canine distemper virus strains reveals the presence of a new genetic variant in South America[J].Virus Genes,2014,48(3):474-478.
[15] Benetka V,Leschnik M,Affenzeller N,et al.Phylogenetic analysis of Austrian canine distemper virus strains from clinical samples from dogs and wild carnivores[J].Vet Rec,2011,168(14):377.
[16] Budaszewski R D F,Pinto L D,Weber M N,et al.Genotyping of canine distemper virus strains circulating in Brazil from 2008 to 2012[J].Virus Res,2014,180:76-83.
[17] Billinis C,Athanasiou L V,Valiakos G,et al.Phylogenetic analysis of canine distemper viruses from red foxes,Greece[J].Vet Rec,2013,173(8):194.
[18] Radtanakatikanon A,Keawcharoen J,Charoenvisal N T,et al.Genotypic lineages and restriction fragment length polymorphism of canine distemper virus isolates in Thailand[J].Vet Microbiol,2013,166(1-2):76-83.
[19] Balboni A,Dandola G D L,Scagliarini A,et al.Occurrence of different Canine distemper virus lineages in Italian dogs[J].Vet Ital,2014,50(3):227-231.
[20] Cha S Y,Kim E J,Kang M,et al.Epidemiology of canine distemper virus in wild raccoon dogs (Nyctereutes procyonoides) from South Korea[J].Comp Immunol Microbiol Infect Dis,2012,35(5):497-504.
[21] Martella V,Cirone F,Elia G,et al.Heterogeneity within the hemagglutinin genes of canine distemper virus (CDV) strains detected in Italy[J]. Vet Microbiol,2006,116(4):301-309.
[22] Zhao J J,Yan X J,Chai X L,et al.Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes,raccoon dogs and minks in China[J].Vet Microbiol,2010,40(1-2):34-42.
[23] 闫如勋,赵建军,柴秀丽,等.狐、貉源犬瘟热病毒 H和F基因的克隆与序列分析[J].江苏农业科学,2012,40(1):23-26.
[24] 朱春生,赵建军,白雪,等.水貂狐狸及貉源犬瘟热病毒 H 基因的克隆与序列分析[J].中国兽医科学,2014,44(2):134-139.
[25] Zhao J J,Zhang H L,Bai X,et al.Emergence of canine distemper virus strains with two amino acid substitutions in the haemagglutinin protein,detected from vaccinated carnivores in North-Eastern China in 2012-2013[J].Vet J,2014,200(1):191-194.
[26] 王磊,王照,李天松,等.小熊猫源犬瘟热病毒全基因序列的克隆及序列分析[J].中国畜牧兽医,2011,38(11):77-81.
[27] Plattet P,Cherpillod P,Wiener D,et al.Signal peptide and helical bundle domains of virulent canine distemper virus fusion protein restrict fusogenicity[J].J Virol,2007,81(20):11413-11425.
[28] 王凤雪,闫喜军,柴秀丽,等.犬瘟热病毒临床流行野毒株 F 蛋白信号肽区分析[J].微生物学通报,2008,35(4):533-538.

水貂、狐狸和貉源犬瘟热病毒F蛋白信号肽区基因克隆及遗传变异分析

Cloning and Genetic Variability Analysis of Single Peptide Gene Regions of Fusion Protein of Canine Distemper Virus from Minks,Foxes and Raccoon Dogs

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	范冰峰, 李文, 刘理想, 赵向远, 邵静, 林乐丰, 孙慧敏, 张旭林, 许保增. 水貂AANAT基因克隆及生物信息学分析[J]. 中国畜牧兽医, 2023, 50(4): 1307-1318.
[2]	唐毓, 杨镒峰, 张颖, 薛海龙, 冯怀亮, 许保增. 貉卵母细胞成熟发育的细胞学研究[J]. 中国畜牧兽医, 2023, 50(4): 1434-1443.
[3]	郭晋祥, 吕会茹, 李姣锋, 廖成水, 张琳琳, 牛俊辉. 甘草查耳酮A对貂源铜绿假单胞菌的抗菌活性[J]. 中国畜牧兽医, 2022, 49(3): 1181-1188.
[4]	宇晓军, 李丹丹, 厉学武, 王群, 王光, 王利华. 抗菌肽对育成期母貂免疫功能及肠组织形态的影响[J]. 中国畜牧兽医, 2022, 49(2): 443-453.
[5]	陈强, 程悦宁, 冯秋菊, 郭利, 张淑琴, 谭斌, 易立, 赵权, 程世鹏, 孙娜. 水貂肺炎克雷伯菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2022, 49(2): 700-708.
[6]	赵静, 褚颖, 罗均, 郭霄峰. 嵌合犬瘟热病毒H基因的重组狂犬病病毒的构建及免疫原性研究[J]. 中国畜牧兽医, 2022, 49(12): 4776-4785.
[7]	刘健, 白艺兰, 鞠厚斌, 潘子豪, 俞向前, 杨显超, 朱晓英, 葛菲菲, 葛杰, 于讳茹, 陶田谷晟, 王建, 赵洪进. 犬瘟热病毒SH202003株全基因组序列分析[J]. 中国畜牧兽医, 2021, 48(7): 2559-2568.
[8]	张庆勋, 景胜凡, 韩姝伊, 王若琪, 冯胜勇, 何宏轩. 水貂源铜绿假单胞菌的分离鉴定、耐药性及毒力基因检测[J]. 中国畜牧兽医, 2021, 48(6): 2230-2237.
[9]	韩玉萍, 赵向远, 范冰峰, 刘理想, 邵静, 许保增. 基于高通量测序的水貂胚胎滞育期和激活期卵巢转录组分析[J]. 中国畜牧兽医, 2021, 48(11): 4035-4046.
[10]	王召阳, 蒋亚君, 刘雪婷, 林伟东, 鑫婷, 侯绍华, 郭晓宇, 朱鸿飞, 贾红. 抗犬瘟热病毒VHH抗体噬菌体库的构建与筛选[J]. 中国畜牧兽医, 2020, 47(6): 1685-1693.
[11]	张新宇, 张海华, 刘晗璐, 钟伟, 司华哲, 韩菲菲, 蔡熙姮, 李光玉. 铜对育成期乌苏里貉生长性能、营养物质消化率的影响[J]. 中国畜牧兽医, 2020, 47(6): 1750-1758.
[12]	宋兴超, 张如, 徐超, 王淑明, 张志明, 杨福合, 彭英华. 水貂毛色性状遗传机理解析及优良品种培育研究进展[J]. 中国畜牧兽医, 2020, 47(6): 1809-1818.
[13]	张明亮, 孙长江, 顾敬敏, 崔子寅, 韩文瑜. 表达铜绿假单胞菌保护性抗原基因F1I2重组鼠伤寒沙门菌在水貂上的免疫原性研究[J]. 中国畜牧兽医, 2020, 47(4): 1156-1162.
[14]	巩文洋, 李漠, 牛一兵, 董雪玉, 李永, 张贵贤, 王夕国, 巩元芳, 苏欣, 杨柳, 邢冠中, 冯敏山, 李素芬. 日粮粗蛋白质和粗脂肪水平对生长貉营养物质消化率和氮代谢的影响[J]. 中国畜牧兽医, 2020, 47(3): 763-770.
[15]	吴洪超, 王凌霄, 霍宁宁, 丁航天, 陈亚磊, 习向锋, 刘友财, 刘玉秀, 田克恭. 新生水貂肠炎病毒母源抗体消长规律及其亚单位疫苗免疫效果研究[J]. 中国畜牧兽医, 2020, 47(11): 3705-3712.