犬瘟热病毒免疫蛋鸡血清抗体与卵黄抗体消长规律的研究

Abstract

Abstract: In order to provide the basis for the preparation of egg yolk antibody,the dynamics of the serum antibody and egg yolk antibody in laying hens against canine distemper virus (CDV) antigen was studied,Vero cell and DF1 cell cultures were inoculated with CDV to detect the virus titer. Select the Vero cytotoxic strain with earlier cytopathic and high virus titers as inoculated virus antigen to immunize laying hens. The immune method was to immunize laying hens once every 10 days, for four times successively, then to strengthen the immune every 30 days. Blood sera were separated every 10 days and egg yolk antibodies were collected every 5 days before and after immunization,the antibody levels were determined by agar diffusion and indirect ELISA. The results showed that serum antibody titer was higher than egg yolk antibody, and both were positively correlated,the appearance of egg yolk antibody was later for 3 to 5 days than serum antibody. Egg yolk antibody had reached the highest level in 3 to 5 days after the third immunity, and then you could start to collect eggs to prepare egg yolk antibody.

Key words: canine distemper virus; serum antibody; egg yolk antibody; dynamics

CLC Number:

S852.4

ZHUANG Jin-qiu, MEI Jian-guo, YAO Chun-yang, SHEN Zhi-qiang, DING Zhuang. Dynamics Research of Serum and Egg Yolk Antibody of Laying Hens Immuned by Canine Distemper Virus[J]. , 2013, 40(9): 214-217.

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Dynamics Research of Serum and Egg Yolk Antibody of Laying Hens Immuned by Canine Distemper Virus

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