猪圆环病毒2型合成肽疫苗佐剂筛选与免疫保护性研究

doi:10.16431/j.cnki.1671-7236.2023.03.022

Abstract

Abstract: 【Objective】 The purpose of this experiment was to screen safer and more efficient adjuvants for Porcine circovirus type 2 (PCV2) synthetic peptide vaccine.【Method】 The adjuvants of IMS 251C VG,GEL 02 PR,ISA 15A VG,ISA 206 VG,ISA 61 VG,Taiwan bacterial flagella,Carbomer,polysaccharide were used to make PCV2 synthetic peptide vaccine according to specific methods.The physical properties of each vaccine were verified,the safety and immune effect of the vaccine were preliminary tested by mouse test,and the better adjuvants were selected for immune challenge protection test of piglets to further evaluate the immune protection effect of each vaccine. 【Result】 The physical properties showed that all vaccines had good stability,and the storage period at 4 ℃ was more than 12 months.In terms of viscosity and emulsion droplet size,ISA 61 VG oil adjuvant was relatively higher and larger,and the two-phase adjuvant of ISA 15A VG and ISA 206 VG were second,the other water adjuvants were lower and smaller.The mouse test results showed that all adjuvants except Taiwan bacterial flagella adjuvant were safe.The results of mouse ELISA antibody level test showed that the immune effect of ISA 61 VG,GEL 02 PR and ISA 206 VG were better than other vaccines.Safety and immune effect were evaluated comprehensively,five adjuvant groups including ISA 61 VG,GEL 02 PR,Carbomer,polysaccharide and ISA 206 VG were selected for immune challenge protection test of piglets, and the results of immune challenge protection test of piglets showed that,the effects of ISA 61 VG and GEL 02 PR were obviously better than other adjuvants.The ELISA antibody and neutralizing antibody level produced by the vaccines prepared by them were significantly higher than those of other groups,and could also stimulate the production of higher levels of cytokine interferon-γ (IFN-γ).After challenge,the relative daily gain of piglets in ISA 61 VG,GEL 02 PR and ISA 206 VG groups was significantly higher than that in other adjuvant groups(P＜0.05),and the results of Real-time PCR and immunohistochemistry showed that the above vaccine groups could significantly reduce the viral load in piglets (P＜0.05).The observation of clinical symptoms showed that the two vaccine groups ISA 61 VG and GEL 02 PR had no temperature rise or roughened hair coats,and the pathological section showed that the pathological changes in the tissues of the two vaccine groups were mild.【Conclusion】 The results showed that the PCV2 synthetic peptide vaccine prepared by oil adjuvant ISA 61 VG and water adjuvant GEL 02 PR had better immune protection against PCV2 infection.This results provided a data basis for the study of PCV2 synthetic peptide vaccine.

Key words: Porcine circovirus type 2 (PCV2); synthetic peptide vaccine; adjuvant screening; immune protection

CLC Number:

S852.65^＋1

LIU Xinyue, LI Lifang, WANG Zhenbao, ZHANG Wenliang, ZHANG Xin, ZHAO Hongtao, LI Pengyu, ZHENG Haotian, XIAO Jin, QI Peng. Adjuvants Screening and Immune Protection of Porcine Circovirus Type 2 Synthetic Peptide Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1068-1080.

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Adjuvants Screening and Immune Protection of Porcine Circovirus Type 2 Synthetic Peptide Vaccine

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