猪流感病毒A/swine/Guangdong/2/2012(H1N1)毒株攻毒BALB/c小鼠细胞因子动态变化的研究

›› 2013, Vol. 40 ›› Issue (10): 11-16.

猪流感病毒A/swine/Guangdong/2/2012(H1N1)毒株攻毒BALB/c小鼠细胞因子动态变化的研究

林文^1,2, 吕艳丽^1,2, 黄良宗², 刘远佳¹, 唐志玲¹, 余绍华¹, 刘明杰², 罗满林¹, 顾万军²

1. 华南农业大学兽医学院, 广东广州 510642;
2. 佛山科学技术学院动物医学系, 广东省高校预防兽医学重点实验室, 广东佛山 528231

收稿日期:2013-03-11 出版日期:2013-10-20 发布日期:2013-12-19
通讯作者: 顾万军 E-mail:guwanjun@163.com
作者简介:林文(1985-),男,广东人,硕士,研究方向:流感病毒。
基金资助:
广东省级动植物防疫检疫科研专项经费。

Dynamic Study of Cytokines in BALB/c Mice Infected with Swine Influenza Virus A/swine/Guangdong/2/2012 (H1N1) Strain

LIN Wen^1,2, LV Yan-li^1,2, HUANG Liang-zong², LIU Yuan-jia¹, TANG Zhi-ling¹, YU Shao-hua¹, LIU Ming-jie², LUO Man-lin¹, GU Wan-jun²

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Key Laboratory of Preventive Veterinary Science in College and Universities of Guangdong Province, Department of Animal Medicine, Foshan Science and Technology University, Foshan 528231, China

Received:2013-03-11 Online:2013-10-20 Published:2013-12-19

摘要/Abstract

摘要： 为了测定H1N1亚型猪流感病毒(swine influenza virus,SIV)对小鼠的致病性,本试验对A/swine/Guangdong/2/2012(H1N1)株SIV HA基因进行克隆及遗传分析,并将SIV尿囊液经鼻腔感染6周龄BALB/c小鼠,观察感染后小鼠的一般状况、器官系数和组织病理学变化,在病毒感染后第1、3和7天使用荧光定量PCR测定小鼠肺脏、脾脏、脑组织中7种细胞因子mRNA的表达量,研究其对小鼠的致病特性。结果显示,该病毒属于经典SIV,病毒经鼻腔感染后可引起小鼠活动减少、采食量降低,但无咳嗽和死亡;病理组织学变化为肺间隔较正常组织明显增厚,毛细血管明显扩张充血,周围肺泡腔呈代偿性肺气肿;小鼠肺脏、脾脏组织样本中IFN-α、IFN-β、IP-10、IL-1β、TNF-α、IRF-3和IL-10 mRNA含量在感染后第3天均显著升高(P<0.05),而脑组织样本中IL-1β和IL-10在小鼠攻毒后第3和7天均显著上调(P<0.05)。

关键词: H1N1亚型猪流感病毒; BALB/c小鼠; 细胞因子

Abstract: To determine the pathogenicity of the H1N1 swine influenza virus (SIV) to mouse, the HA gene of A/swine/Guangdong/2/2012(H1N1) SIV was cloned,and then genetic analysis was made. The allantoic fluid containing SIV was given to BALB/c mouse that was 6 to 8 months old by nasal infection. General condition, viscera index and histopathological changes of mice were observed and the expression level of 7 kinds of cytokines in the lung, spleen and brain of mice were determined by fluorescence quantitative PCR method at 1,3 and 7 d after infection to determine the pathogenicity. The results revealed that the virus in this study was found to be classical SIV for that it could cause the decline of the activity and feed intake in mice,but no cough and death were showed. Obvious increase was showed in lung interval than the normal tissue. Expansion of congestion was showed in blood capillary, compensatory emphysema could be seen in the surrounding alveolar cavity as well. In the third day after infection,the content of mRNA of cytokines IFN-α,IFN-β,IP-10,IL-1β,TNF-α,IRF-3 and IL-10 showed obvious rising trends while cytokines IL-1β and IL-10 in brain showed the same trend at the third and seventh day after infection.

Key words: H1N1 subtype swine influenza virus; BALB/c mice; cytokine

中图分类号:

Q813.1⁺1

林文, 吕艳丽, 黄良宗, 刘远佳, 唐志玲, 余绍华, 刘明杰, 罗满林, 顾万军. 猪流感病毒A/swine/Guangdong/2/2012(H1N1)毒株攻毒BALB/c小鼠细胞因子动态变化的研究[J]. , 2013, 40(10): 11-16.

LIN Wen, LV Yan-li, HUANG Liang-zong, LIU Yuan-jia, TANG Zhi-ling, YU Shao-hua, LIU Ming-jie, LUO Man-lin, GU Wan-jun. Dynamic Study of Cytokines in BALB/c Mice Infected with Swine Influenza Virus A/swine/Guangdong/2/2012 (H1N1) Strain[J]. , 2013, 40(10): 11-16.

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