亚临床酮病对泌乳早期奶牛繁殖性能和血液生化指标的影响

doi:10.16431/j.cnki.1671-7236.2020.01.034

摘要/Abstract

摘要： 试验旨在探究围产期亚临床酮病与泌乳早期奶牛繁殖性能、卵泡发育之间的关系，并检测试验牛血液生化指标的变化。试验在黑龙江某大型集约化牛场开展，根据产后血酮水平确定亚临床酮病组（SCK）和健康组（C）奶牛共60头，根据试验牛产后50 d内发情状况，将SCK组再分为发情组（SCKE，16头）和乏情组（SCKA，14头），C组也同样分为发情组（CE，25头）和乏情组（CA，5头）。所有试验牛在产后50 d通过直肠检查和B超检查了解子宫复旧及卵泡发育状况，记录繁殖性能数据，并进行血液生化指标分析。结果表明：与健康组发情奶牛相比，亚临床酮病发情奶牛产后首次发情日期推迟约10 d（P<0.05）；产后50 d卵泡直径差异极显著（差值约4 mm）（P<0.01）。亚临床酮病乏情奶牛子宫复旧延迟发生率显著高于健康组发情奶牛（P<0.05）；亚临床酮病奶牛血浆中胰岛素样生长因子-1（IGF-1）含量显著低于健康奶牛（P<0.05），而泌乳量极显著提高（P<0.01）。与发情奶牛相比，乏情奶牛血浆中甘油三酯（TG）含量显著升高（P<0.05）；葡萄糖（Glu）、胰岛素（Ins）、雌二醇（E₂）、孕酮（P₄）含量显著或极显著降低（P<0.05；P<0.01）。综合以上试验结果，奶牛患亚临床酮病而导致能量代谢指标异常是引起奶牛乏情、产后卵泡发育受阻和繁殖障碍的主要因素，进而导致奶牛繁殖力下降。

关键词: 亚临床酮病; 奶牛; 乏情; 繁殖性能; 卵泡发育

Abstract: The aim of this study was to explore the relationship between perinatal subclinical ketosis,reproductive performance and follicular development in early lactation,and to detect changes in blood biochemical of the dairy cows.60 cows from a large intensive dairy farm in Heilongjiang province were assigned to subclinical ketosis group (SCK) and control health group (C) according to ketone bodies levels postpartum.According to the estrus condition within 50 d in milk,16 cows and 14 cows of the SCK group was further divided into the estrus group (SCKE) and the anestrus group (SCKA),respectively.The cows in group C was also divided into estrus group (CE,25 cows) and anestrus group (CA,5 cows).All groups of cows were tracked to record reproductive performance data and follicular development status at 50 d postpartum by rectal examination and B-ultrasound.Results showed that the first estrus days of SCKE cows were 10 d longer than the CE cows (P<0.05),and the difference of diameter of follicles was more than 4 mm (P<0.01).The incidence of uterine incompleteness in SCKA group was significantly higher than that in CE group (P<0.05).The plasma insulin-like growth factor-1 (IGF-1) level was significantly decreased (P<0.05) and the milk yield was extremely significantly increased in SCK group (P<0.01).Compared with estrus cows,the content of triglyceride (TG) in plasma of anestrus cows was increased significantly (P<0.05);The contents of glucose (Glu),insulin (Ins),estradiol (E₂) and progesterone (P₄) decreased significantly or extremely significantly (P<0.05;P<0.01).In conclusion,abnormal energy metabolism and reproductive hormone in cows with subclinical ketosis are the risk factor for reproductive and follicles development disorder postpartum in dairy cows,which will lead to poor fertility in dairy cows.

Key words: subclinical ketosis; dairy cow; anestrus; reproductive performance; follicular development

中图分类号:

S852.35

张锋, 白云龙, 宋玉锡, 孙书函, 夏成, 徐闯, 张洪友. 亚临床酮病对泌乳早期奶牛繁殖性能和血液生化指标的影响[J]. 中国畜牧兽医, 2020, 47(1): 290-295.

ZHANG Feng, BAI Yunlong, Song Yuxi, SUN Shuhan, XIA Cheng, XU Chuang, ZHANG Hongyou. Effect of Subclinical Ketosis on Reproductive Performance and Blood Clinical Pathology of Dairy Cows During Early Lactation[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 290-295.

参考文献

[1] 韩东.奶牛亚临床酮病的发病原因、临床表现及防治措施[J].现代畜牧科技,2017,2:132. HAN D.Clinical symptom and prevention measures of subclinical ketosis in dairy cows[J].Modern Animal Husbandry Science and Technology,2017,2:132.(in Chinese)
[2] JEONG J K,CHOI I S,MOON S H,et al.Effect of two treatment protocols for ketosis on the resolution,postpartum health,milk yield,and reproductive outcomes of dairy cows[J].Theriogenology,2017,36(5):1635-1643.
[3] RUTHERFORD A J,OIKONOMOU G,SMITH R F.The effect of subclinical ketosis on activity at estrus and reproductive performance in dairy cattle[J].Journal of Dairy Science,2016,99(6):4808-4815.
[4] 扈光辉,扈光强,郑鹏,等.不同因素对初产奶牛繁殖力的影响[J].江苏农业科学,2017,45(4):128-130. HU G H,HU G Q,ZHENG P,et al.Effects of different factors on the reproductive capacity of primitive dairy cows[J].Jiangsu Agricultural Sciences,2017,45(4):128-130.(in Chinese)
[5] DE RENSIS F,LOPEZ-GATIUS F,GARCIA-ISPIERTO I,et al.Causes of declining fertility in dairy cows during the warm season[J].Theriogenology,2017,91:145-153.
[6] 夏成,王洪斌,张洪友,等.能量负平衡对泌乳早期奶牛生产性能、繁殖性能和机体代谢的影响[J].中国畜牧杂志,2009,45(21):32-35. XIA C,WANG H B,ZHANG H Y,et al.Effects of negative energy balance on production performance,reproductive performance and body metabolism of early lactation dairy cows[J].Chinese Journal of Animal Husbandry,2009,45(21):32-35.(in Chinese)
[7] BICALHO M L S,MARQUES E C,GILBERT R O,et al.The association of plasma glucose,BHBA,and NEFA with postpartum uterine diseases,fertility,and milk production of Holstein dairy cows[J].Theriogenology,2016,85(9):3185-3193.
[8] 钱伟东.高产奶牛产后主要卵巢疾病的调查和血液临床病理学变化及其预警评估[D].大庆:黑龙江八一农垦大学,2016. QIAN W D.Investigation of major postpartum ovarian diseases and blood clinical pathological changes in high production cows and their early warning assessment[D].Daqing:Heilongjiang Bayi Agricultural University,2016.(in Chinese)
[9] RIBEIRO E S,LIMA F S,GRECO L F,et al.Prevalence of periparturient diseases and effects on fertility of seasonally calving grazing dairy cows supplemented with concentrates[J].Journal of Dairy Science,2013,96(9):5682-5697.
[10] AUNGIER S P M,ROCHE J F,DISKIN M G,et al.Risk factors that affect reproductive target achievement in fertile dairy cows[J].Journal of Dairy Science,2014,97(6):3472-3487.
[11] 张江,孙书函,赵畅,等.黑龙江省某集约化牛场酮病奶牛血液生化指标变化[J].畜牧与兽医,2018,15:69-72. ZHANG J,SUN S H,ZHAO C,et al.Changes in blood biochemical parameters of an intensive cattle ketone disease cow in Heilongjiang province[J].Animal Husbandry and Veterinary Medicine,2018,15:69-72.(in Chinese)
[12] LEROY J L,VANHOLDER T,OPSOMER G,et al.The in vitro development of bovine oocytes after maturation in glucose and beta-hydroxybutyrate concentrations associated with negative energy balance in dairy cows[J].Reproduction in Domestic Animals,2010,41(2):119-123.
[13] SANTOS J E P,BISINOTTO R S,RIBEIRO E S.Mechanisms underlying reduced fertility in anovular dairy cows[J].Theriogenology,2016,58(7):1632-1639.
[14] SANTOS J E,BISINOTTO R S,RIBEIRO E S.Mechanisms underlying reduced fertility in anovular dairy cows[J].Theriogenology,2016,86(1):254-262.
[15] RUTHERFORD A J,OIKONOMOU G,SMITH R F.The effect of subclinical ketosis on activity at estrus and reproductive performance in dairy cattle[J].Journal of Dairy Science,2016,99(6):4808-4815.
[16] SUTHAR V S,CANELAS-RAPOSO J,DENIZ A,et al.Prevalence of subclinical ketosis and relationships with postpartum diseases in European dairy cows[J].Journal of Dairy Science,2013,96(5):2925-2938.
[17] DUBUC J,DUFFIELD T F,LESLIE K E,et al.Risk factors for postpartum uterine diseases in dairy cows[J].Journal of Dairy Science,2010,93(12):5764-5771.
[18] 田宏志,刘江静,陈晓丽,等.奶牛生殖激素和体温的性周期变化及调控机制研究进展[J].中国畜牧杂志,2019,55(2):32-37. TIAN H Z,LIU J J,CHEN X L,et al.Progress in sexual cycle changes and regulation mechanisms of reproductive hormone and body temperature in dairy cows[J].Chinese Journal of Animal Science,2019,55(2):32-37.(in Chinese)
[19] GOBIKRUSHANTH M,PURFIELD D C,COLAZO M G,et al.The relationship between serum anti-Mullerian hormone concentrations and fertility,and genome-wide associations for anti-Mullerian hormone in Holstein cows[J].Journal of Dairy Science,2018,101(8):5163-5171.
[20] GOBIKRUSHANTH M,PURFIELD D C,COLAZO M G,et al.The relationship between serum insulin-like growth factor-1(IGF-1) concentration and reproductive performance,and genome-wide associations for serum IGF-1 in Holstein cows[J].Journal of Dairy Science,2018,122(7):74-83.
[21] MARESCA S,VALIENTE S O L,RODRIGUEZ A M,et al.Effect of protein restriction of bovine dams during late gestation on offspring postnatal growth,glucose-insulin metabolism and IGF-1 concentration[J].Livestock Science,2018,45(11),1796-1802.