犊牛肺源致病性大肠杆菌分离鉴定、耐药性及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2020.01.028

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (1): 240-248.doi: 10.16431/j.cnki.1671-7236.2020.01.028

犊牛肺源致病性大肠杆菌分离鉴定、耐药性及耐药基因检测

顾晓晓¹, 邬琴¹, 陶乔孝慈¹, 马雪¹, 李劼¹, 周霞¹, 韩猛立², 黄新², 吴桐忠², 张星星², 钟发刚²

1. 石河子大学动物科技学院, 石河子 832003;
2. 新疆农垦科学院, 省部共建绵羊遗传改良与健康养殖国家重点实验室, 石河子 832000

收稿日期:2019-08-02 出版日期:2020-01-20 发布日期:2020-01-17
通讯作者: 周霞, 韩猛立 E-mail:32123004@qq.com;605249596@qq.com
作者简介:顾晓晓(1993-),女,河南商丘人,硕士生,研究方向:兽医微生物与免疫学,E-mail:1085483237@qq.com
基金资助:
省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项项目（SKLSGIHP2016A02、SKLSGIHP2017A03）；兵团区域创新引导计划（2017BA038、2018BB036）；兵团重大科技攻关项目（2014AA001-3）；兵团重大科技项目（2017AA006-04）；兵团重点领域科技攻关计划（2019AB029）

Isolation,Identification and Detection of Drug Resistance and Drug Resistance Gene of Pulmonary Pathogenic Escherichia coli from Calves

GU Xiaoxiao¹, WU Qin¹, TAO Qiaoxiaoci¹, MA Xue¹, LI Jie¹, ZHOU Xia¹, HAN Mengli², HUANG Xin², WU Tongzhong², ZHANG Xingxing², ZHONG Fagang²

1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
2. National Key Laboratory of Sheep Genetic Improvement and Healthy Aquaculture, Xinjiang Academy of Agricultural Reclamation, Shihezi 832000, China

Received:2019-08-02 Online:2020-01-20 Published:2020-01-17

摘要/Abstract

摘要： 本试验旨在确定新疆石河子地区某牛场因呼吸系统疾病导致犊牛死亡的细菌性病原，并对分离病原进行系统进化分类、耐药表型和耐药基因分析。采用细菌常规分离结合全自动生化分析系统对分离株进行分离鉴定，采用纸片法和PCR方法对分离株进行系统分群、药敏表型及耐药基因的分析。从患病犊牛肺脏、肝脏及淋巴结组织分离鉴定出6株致病性大肠杆菌，6株分离株均呈多重耐药现象，其中对青霉素、阿莫西林、头孢唑啉、头孢拉定、链霉素、庆大霉素、氟苯尼考、替考拉宁、克林霉素、美罗培南、四环素和妥布霉素的耐药率高达100%。氨基糖苷类耐药基因aacC2、β-内酰胺类耐药基因bla_CTX-M和bla_TEM、四环素类耐药基因tetA、喹诺酮类耐药基因gyrA、磺胺类耐药基因sul2携带率分别为66.67%、83.3%、66.7%、100%、100%和100%。结果表明，新疆石河子某牛场发生呼吸道感染死亡犊牛细菌性病原是大肠杆菌，且表现较强的多重耐药现象，分离株携带更为丰富的耐药基因。

关键词: 牛呼吸系统感染; 致病性大肠杆菌; 耐药表型; 耐药基因

Abstract: The purpose of this study was to identify the bacterial pathogens that cause the death of calves due to respiratory diseases in a cattle farm in Shihezi,Xinjiang,and to analyze the phylogenetic classification,drug resistance phenotype and drug resistance gene of the isolated pathogens.The routine isolation of bacteria combined with automatic biochemical analysis system was used to isolate and identify the isolates.The paper and PCR methods were used to analyze the systematic grouping,drug sensitive phenotype and drug resistance genes of the isolates.Six strains of pathogenic E.coli were isolated and identified from the lung,liver and lymph nodes of infected calves.All of the six strains were multidrug resistant.The drug resistance rates of penicillin,amoxicillin,cefazolin,cefradine,streptomycin,gentamicin,florfenicol,teicoplanin,clindamycin,meropenem,tetracycline and tobramycin were 100%.The carrying rates of aminoglycoside resistance genes aacC2,β-lactam resistance genes bla_CTX-M and bla_TEM,tetracycline resistance gene tetA,quinolone resistance gene gyrA,and sulfonamide resistance gene sul2 were 66.67%,83.3%,66.7%,100%,100% and 100%,respectively.E.coli was the bacterial pathogen causing respiratory tract infections in dead calves in a cattle farm in Shihezi,Xinjiang.It showed strong multi-drug resistance,and the isolates carried more abundant drug resistance genes.

Key words: bovine respiratory infection; pathogenic Escherichia coli; drug resistance; drug resistance gene

中图分类号:

S852.61

顾晓晓, 邬琴, 陶乔孝慈, 马雪, 李劼, 周霞, 韩猛立, 黄新, 吴桐忠, 张星星, 钟发刚. 犊牛肺源致病性大肠杆菌分离鉴定、耐药性及耐药基因检测[J]. 中国畜牧兽医, 2020, 47(1): 240-248.

GU Xiaoxiao, WU Qin, TAO Qiaoxiaoci, MA Xue, LI Jie, ZHOU Xia, HAN Mengli, HUANG Xin, WU Tongzhong, ZHANG Xingxing, ZHONG Fagang. Isolation,Identification and Detection of Drug Resistance and Drug Resistance Gene of Pulmonary Pathogenic Escherichia coli from Calves[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 240-248.

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犊牛肺源致病性大肠杆菌分离鉴定、耐药性及耐药基因检测

Isolation,Identification and Detection of Drug Resistance and Drug Resistance Gene of Pulmonary Pathogenic Escherichia coli from Calves

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