FADS2基因在奶牛乳腺细胞中的过表达和干扰研究

doi:10.16431/j.cnki.1671-7236.2019.03.002

Abstract

Abstract:

In order to explore the role of fatty acid desaturases 2 (FADS2) gene in fatty acid metabolism in dairy mammary gland cells,this study overexpressed and interfered with FADS2 gene in dairy mammary epithelial cells,and studied the regulation of FADS2 gene on fatty acid synthesis related genes and the effect on triglyceride content in dairy mammary epithelial cells.The siRNA and overexpression vector pcDNA3.1-FADS2-EGFP were designed according to the CDS sequence of FADS2 gene.The effect of FADS2 gene overexpression and interference on the expression of fatty acid metabolism related genes and the change of triglyceride content in cells were detected by transfecting mammary gland cells.The results showed that the overexpression vector pcDNA3.1-FADS2-EGFP and the interference fragment were successfully obtained,and the transfected cells had good overexpression and interference effects.After overexpression of FADS2 gene,AGPAT1,SCAP,GPAM,ELOVL5,ACAA1,FADS1,DGAT1 and PPARα genes were significantly down-regulated (P<0.05),and PLIN2 gene was extremely significantly up-regulated (P<0.01).After FADS2 gene interference,AGPAT1,GPAM,ELOVL5,ACAA1,PLIN2 and FADS1 genes were significantly up-regulated (P<0.05),INSIG1 gene was extremely significantly up-regulated (P<0.01),andDGAT1 and PPARα genes were significantly down-regulated (P<0.05).Triglyceride assay showed that FADS2 gene overexpression and interference could reduce the content of triglyceride in mammary gland epithelial cells.In summary,FADS2 gene could regulate the expression of lipid synthesis-related genes in mammary gland epithelial cells,and had a regulatory effect on breast lipid synthesis.

Key words: mammary epithelial cells; FADS2 gene; overexpression; interfere; fatty acid synthesis

CLC Number:

S823.9⁺1

MA Xiaoya, PANG Chunying, DENG Tingxian, DUAN Anqin, LU Xingrong, LIANG Shasha, LIANG Xianwei. Overexpression and Interference of FADS2 Gene in Mammary Gland Cells of Dairy Cows[J]. , 2019, 46(3): 652-660.

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Overexpression and Interference of FADS2 Gene in Mammary Gland Cells of Dairy Cows

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