重组鸡白细胞介素-6/2融合蛋白对新城疫(LaSota株)活疫苗免疫增强作用研究

doi:10.16431/j.cnki.1671-7236.2019.02.031

Abstract

Abstract:

In order to study the immunopotentiation effect of recombinant chicken interleukin-6/2 fusion protein (rChIL-6-Linker-ChIL-2) on Newcastle disease vinus (NDV) live vaccine (LaSota vaccine),90 SPF chickens were chosen and randomly distributed into six groups which were the PBS control group,NDV live vaccine control group,rChIL-6-Linker-ChIL-2 immunopotentiation group,rChIL-6 protein immunopotentiation group,rChIL-2 protein immunopotentiation group and the rChIL-6+rChIL-2 fusion protein immunopotentiation group.The interleukin fusion protein fluid and NDV live vaccine (LaSota vaccine) were inoculated into SPF chickens.The indexes of proliferation activities of peripheral blood and splenic lymphocytes,percentages of the CD3⁺CD4⁺/CD3⁺CD8⁺ T-lymphocytes in peripheral blood,the contents of Th1/Th2-type cytokines and the specific immune antibody level were detected by assays of MTS,FCM,ELISA and HA/HI.The results showed that compared with the indexes of NDV vaccine inoculation control group,the lymphocytes proliferation activity,the percentage of CD3⁺CD4⁺/CD3⁺CD8⁺ T-lymphocytes,and the contents of Th1/Th2-type cytokines such as ChIL-4,ChIL-6,ChIL-2,ChIFN-α and ChIFN-γ of chickens in rChIL-6-Linker-ChIL-2 inoculation group were promoted obviously at 7 to 21 dpi,and the HI immune antibodies increased about 1.0 to 1.9 titer,which was 0.2 to 0.7 titer higher than that of rChIL-6 group and 0.2 to 1.1 titer higher than rChIL-2 that of group.In conclusion,the rChIL-6-Linker-ChIL-2 protein had an obvious immunopotentiation effect on NDV live vaccine (LaSota) in chickens.

Key words: rChIL-6-Linker-ChIL-2; immunopotentiation effect; proliferation activity of lymphocytes; Th1/Th2-type cytokines; antibody titer

CLC Number:

S852.4

LIU Meifen, LI Qinnan, MA Zhenyuan, WU Zhiming, BAN Fuguo, YAN Ruoqian, WANG Huajun. Immunopotentiation Effect of Recombinant Chicken Interleukin-6/2 Fusion Protein on Newcastle Disease Virus (LaSota) Live Vaccine[J]. , 2019, 46(2): 590-599.

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Immunopotentiation Effect of Recombinant Chicken Interleukin-6/2 Fusion Protein on Newcastle Disease Virus (LaSota) Live Vaccine

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