›› 2017, Vol. 44 ›› Issue (10): 2944-2950.doi: 10.16431/j.cnki.1671-7236.2017.10.016

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Optimization of Fermentation Conditions for Corn Gluten Meal by Mixed Strains

WEI Bing-dong1, MIAO Guo-wei2, CHEN Qun1, QIU Yu-lang1, LI Lin1, YU Wei1, LI Wei-zhong3   

  1. 1. Branch Institute of Animal Science, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China;
    2. Jilin Agricultural University, Changchun 130033, China;
    3. Weifang University, Weifang 261061, China
  • Received:2016-12-06 Online:2017-10-20 Published:2017-10-20

Abstract:

The objective of this experimentation was to optimizate the fermentation conditions for corn gluten meal by four different bacterial strains (Aspergillus niger,Lactobacillus,yeast and Bacillus licheniformis) through the acclimatization test of strains for hydrolysis corn gluten meal,orthogonal experimental of strains proportion determination and fermentation condition (temperature,time,moisture content and initial pH) and taking the peptides yield rate as an index.And then the crude protein,yield of peptides,total sugar,reducing sugar and amino acid constitute of corn gluten meal fermented at the optimal condition were determined. The results showed that the inoculation proportion of Bacillus licheniformis,Lactobacillus,yeast and Aspergillus niger were 4%,4%,6% and 6%,respectively. The optimal conditions for fermentation was that the fermentation temperature,fermentation time,moisture content and initial pH were 35℃,84 h,45% and 6,respectively,and the peptides yield rate was 44.03%.During fermentation,the crude protein content and peptides yield were increased.At the end of the fermentation,the content of crude protein,peptides yield rate,reducing sugar,total essential amino acid and total amino acid and were increased by 15.17%,205%,850%,12.58% and 12.37%,respectively.

Key words: mixed strains; corn gluten meal; fermentation; conditions optimization

CLC Number: